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Hyper sensitive protein detection by Tandem-HTRF reveals Cyclin D1 dynamics in adult mouse
We present here a novel method for the semi-quantitative detection of low abundance proteins in solution that is both fast and simple. It is based on Homogenous Time Resolved Förster Resonance Energy Transfer (HTRF), between a lanthanide labeled donor antibody and a d2 or XL665 labeled acceptor anti...
Autores principales: | Zampieri, Alexandre, Champagne, Julien, Auzemery, Baptiste, Fuentes, Ivanna, Maurel, Benjamin, Bienvenu, Frédéric |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622077/ https://www.ncbi.nlm.nih.gov/pubmed/26503526 http://dx.doi.org/10.1038/srep15739 |
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