Cargando…

The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective

Botulinum neurotoxins (BoNTs) produced by the anaerobic bacterium Clostridium botulinum are the most poisonous substances known to mankind. However, toxin regulation and signals triggering synthesis as well as the regulatory network and actors controlling toxin production are unknown. Experiments sh...

Descripción completa

Detalles Bibliográficos
Autores principales: Ihekwaba, Adaoha E. C., Mura, Ivan, Peck, Michael W., Barker, G. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622173/
https://www.ncbi.nlm.nih.gov/pubmed/26449712
http://dx.doi.org/10.1093/femspd/ftv084
_version_ 1782397554368970752
author Ihekwaba, Adaoha E. C.
Mura, Ivan
Peck, Michael W.
Barker, G. C.
author_facet Ihekwaba, Adaoha E. C.
Mura, Ivan
Peck, Michael W.
Barker, G. C.
author_sort Ihekwaba, Adaoha E. C.
collection PubMed
description Botulinum neurotoxins (BoNTs) produced by the anaerobic bacterium Clostridium botulinum are the most poisonous substances known to mankind. However, toxin regulation and signals triggering synthesis as well as the regulatory network and actors controlling toxin production are unknown. Experiments show that the neurotoxin gene is growth phase dependent for C. botulinum type A1 strain ATCC 19397, and toxin production is influenced both by culture conditions and nutritional status of the medium. Building mathematical models to describe the genetic and molecular machinery that drives the synthesis and release of BoNT requires a simultaneous description of the growth of the bacterium in culture. Here, we show four plausible modelling options which could be considered when constructing models describing the pattern of growth observed in a botulinum growth medium. Commonly used bacterial growth models are unsuitable to fit the pattern of growth observed, since they only include monotonic growth behaviour. We find that a model that includes both the nutritional status and the ability of the cells to sense their surroundings in a quorum-sensing manner is most successful at explaining the pattern of growth obtained for C. botulinum type A1 strain ATCC 19397.
format Online
Article
Text
id pubmed-4622173
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-46221732015-10-28 The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective Ihekwaba, Adaoha E. C. Mura, Ivan Peck, Michael W. Barker, G. C. Pathog Dis Research Article Botulinum neurotoxins (BoNTs) produced by the anaerobic bacterium Clostridium botulinum are the most poisonous substances known to mankind. However, toxin regulation and signals triggering synthesis as well as the regulatory network and actors controlling toxin production are unknown. Experiments show that the neurotoxin gene is growth phase dependent for C. botulinum type A1 strain ATCC 19397, and toxin production is influenced both by culture conditions and nutritional status of the medium. Building mathematical models to describe the genetic and molecular machinery that drives the synthesis and release of BoNT requires a simultaneous description of the growth of the bacterium in culture. Here, we show four plausible modelling options which could be considered when constructing models describing the pattern of growth observed in a botulinum growth medium. Commonly used bacterial growth models are unsuitable to fit the pattern of growth observed, since they only include monotonic growth behaviour. We find that a model that includes both the nutritional status and the ability of the cells to sense their surroundings in a quorum-sensing manner is most successful at explaining the pattern of growth obtained for C. botulinum type A1 strain ATCC 19397. Oxford University Press 2015-10-07 2015-12 /pmc/articles/PMC4622173/ /pubmed/26449712 http://dx.doi.org/10.1093/femspd/ftv084 Text en © FEMS 2015. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ihekwaba, Adaoha E. C.
Mura, Ivan
Peck, Michael W.
Barker, G. C.
The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective
title The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective
title_full The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective
title_fullStr The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective
title_full_unstemmed The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective
title_short The pattern of growth observed for Clostridium botulinum type A1 strain ATCC 19397 is influenced by nutritional status and quorum sensing: a modelling perspective
title_sort pattern of growth observed for clostridium botulinum type a1 strain atcc 19397 is influenced by nutritional status and quorum sensing: a modelling perspective
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622173/
https://www.ncbi.nlm.nih.gov/pubmed/26449712
http://dx.doi.org/10.1093/femspd/ftv084
work_keys_str_mv AT ihekwabaadaohaec thepatternofgrowthobservedforclostridiumbotulinumtypea1strainatcc19397isinfluencedbynutritionalstatusandquorumsensingamodellingperspective
AT muraivan thepatternofgrowthobservedforclostridiumbotulinumtypea1strainatcc19397isinfluencedbynutritionalstatusandquorumsensingamodellingperspective
AT peckmichaelw thepatternofgrowthobservedforclostridiumbotulinumtypea1strainatcc19397isinfluencedbynutritionalstatusandquorumsensingamodellingperspective
AT barkergc thepatternofgrowthobservedforclostridiumbotulinumtypea1strainatcc19397isinfluencedbynutritionalstatusandquorumsensingamodellingperspective
AT ihekwabaadaohaec patternofgrowthobservedforclostridiumbotulinumtypea1strainatcc19397isinfluencedbynutritionalstatusandquorumsensingamodellingperspective
AT muraivan patternofgrowthobservedforclostridiumbotulinumtypea1strainatcc19397isinfluencedbynutritionalstatusandquorumsensingamodellingperspective
AT peckmichaelw patternofgrowthobservedforclostridiumbotulinumtypea1strainatcc19397isinfluencedbynutritionalstatusandquorumsensingamodellingperspective
AT barkergc patternofgrowthobservedforclostridiumbotulinumtypea1strainatcc19397isinfluencedbynutritionalstatusandquorumsensingamodellingperspective