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Glycated nail proteins as a new biomarker in management of the South Kivu Congolese diabetics

INTRODUCTION: Diagnosis and monitoring of diabetes mellitus in sub-Saharan Africa, based on blood analyses, are hampered by infrastructural and cultural reasons. The first aim of this study was to evaluate the diagnostic accuracy of glycated nail proteins for diabetes mellitus. The second aim was to...

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Autores principales: Katchunga, Philippe B., Mirindi, Patrick N., Kishabongo, Antoine S., Cikomola, Justin C., Bwanamdogo, Socrate, Philippé, Jan, Speeckaert, Marijn M., Delanghe, Joris R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Croatian Society of Medical Biochemistry and Laboratory Medicine 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622194/
https://www.ncbi.nlm.nih.gov/pubmed/26526975
http://dx.doi.org/10.11613/BM.2015.048
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author Katchunga, Philippe B.
Mirindi, Patrick N.
Kishabongo, Antoine S.
Cikomola, Justin C.
Bwanamdogo, Socrate
Philippé, Jan
Speeckaert, Marijn M.
Delanghe, Joris R.
author_facet Katchunga, Philippe B.
Mirindi, Patrick N.
Kishabongo, Antoine S.
Cikomola, Justin C.
Bwanamdogo, Socrate
Philippé, Jan
Speeckaert, Marijn M.
Delanghe, Joris R.
author_sort Katchunga, Philippe B.
collection PubMed
description INTRODUCTION: Diagnosis and monitoring of diabetes mellitus in sub-Saharan Africa, based on blood analyses, are hampered by infrastructural and cultural reasons. The first aim of this study was to evaluate the diagnostic accuracy of glycated nail proteins for diabetes mellitus. The second aim was to compare the course of short- and long-term glycemic biomarkers after 6 months of antidiabetic treatment. These objectives should support our hypothesis that glycated nail proteins could be used as an alternative glycemic biomarker. MATERIALS AND METHODS: This case-control study consisted of 163 black diabetics and 67 non-diabetics of the South Kivu (Democratic Republic of Congo). Diagnostic accuracy of glycated nail proteins was evaluated using ROC curve analysis. At the start of the study, glycated nail protein concentrations were compared between diabetics and non-diabetics, using a nitro blue tetrazolium (NBT) colorimetric method. In a subgroup of 30 diabetics, concentrations of glycated nail proteins, fasting glucose (Accu-Chek® Aviva), serum fructosamine (NBT) and HbA1c (DCA-2000+®) were measured at start and after 6 months. RESULTS: ROC analysis yielded an AUC of 0.71 (95% confidence interval (CI): 0.65-0.76) and a cut-off point of 3.83 μmol/g nail. Concentration of glycated nail proteins was significantly higher (P < 0.001) in diabetics in comparison with non-diabetics. After 6 months of antidiabetic treatment, a significant drop in the fasting glucose concentration (P = 0.017) and concentration of glycated nail proteins (P = 0.008) was observed in contrast to serum fructosamine and HbA1c. CONCLUSIONS: Measurement of glycated nail proteins could be used to diagnose and monitor diabetes mellitus in sub-Saharan Africa.
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spelling pubmed-46221942015-10-30 Glycated nail proteins as a new biomarker in management of the South Kivu Congolese diabetics Katchunga, Philippe B. Mirindi, Patrick N. Kishabongo, Antoine S. Cikomola, Justin C. Bwanamdogo, Socrate Philippé, Jan Speeckaert, Marijn M. Delanghe, Joris R. Biochem Med (Zagreb) Research Article INTRODUCTION: Diagnosis and monitoring of diabetes mellitus in sub-Saharan Africa, based on blood analyses, are hampered by infrastructural and cultural reasons. The first aim of this study was to evaluate the diagnostic accuracy of glycated nail proteins for diabetes mellitus. The second aim was to compare the course of short- and long-term glycemic biomarkers after 6 months of antidiabetic treatment. These objectives should support our hypothesis that glycated nail proteins could be used as an alternative glycemic biomarker. MATERIALS AND METHODS: This case-control study consisted of 163 black diabetics and 67 non-diabetics of the South Kivu (Democratic Republic of Congo). Diagnostic accuracy of glycated nail proteins was evaluated using ROC curve analysis. At the start of the study, glycated nail protein concentrations were compared between diabetics and non-diabetics, using a nitro blue tetrazolium (NBT) colorimetric method. In a subgroup of 30 diabetics, concentrations of glycated nail proteins, fasting glucose (Accu-Chek® Aviva), serum fructosamine (NBT) and HbA1c (DCA-2000+®) were measured at start and after 6 months. RESULTS: ROC analysis yielded an AUC of 0.71 (95% confidence interval (CI): 0.65-0.76) and a cut-off point of 3.83 μmol/g nail. Concentration of glycated nail proteins was significantly higher (P < 0.001) in diabetics in comparison with non-diabetics. After 6 months of antidiabetic treatment, a significant drop in the fasting glucose concentration (P = 0.017) and concentration of glycated nail proteins (P = 0.008) was observed in contrast to serum fructosamine and HbA1c. CONCLUSIONS: Measurement of glycated nail proteins could be used to diagnose and monitor diabetes mellitus in sub-Saharan Africa. Croatian Society of Medical Biochemistry and Laboratory Medicine 2015-10-15 /pmc/articles/PMC4622194/ /pubmed/26526975 http://dx.doi.org/10.11613/BM.2015.048 Text en
spellingShingle Research Article
Katchunga, Philippe B.
Mirindi, Patrick N.
Kishabongo, Antoine S.
Cikomola, Justin C.
Bwanamdogo, Socrate
Philippé, Jan
Speeckaert, Marijn M.
Delanghe, Joris R.
Glycated nail proteins as a new biomarker in management of the South Kivu Congolese diabetics
title Glycated nail proteins as a new biomarker in management of the South Kivu Congolese diabetics
title_full Glycated nail proteins as a new biomarker in management of the South Kivu Congolese diabetics
title_fullStr Glycated nail proteins as a new biomarker in management of the South Kivu Congolese diabetics
title_full_unstemmed Glycated nail proteins as a new biomarker in management of the South Kivu Congolese diabetics
title_short Glycated nail proteins as a new biomarker in management of the South Kivu Congolese diabetics
title_sort glycated nail proteins as a new biomarker in management of the south kivu congolese diabetics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622194/
https://www.ncbi.nlm.nih.gov/pubmed/26526975
http://dx.doi.org/10.11613/BM.2015.048
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