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In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications

Recent advances in theranostic nanomedicine can promote stem cell and immune cell-based therapy. Gold nanoparticles (GNPs) have been shown to be promising agents for in-vivo cell-tracking in cell-based therapy applications. Yet a crucial challenge is to develop a reliable protocol for cell upload wi...

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Autores principales: Betzer, Oshra, Meir, Rinat, Dreifuss, Tamar, Shamalov, Katerina, Motiei, Menachem, Shwartz, Amit, Baranes, Koby, Cohen, Cyrille J., Shraga-Heled, Niva, Ofir, Racheli, Yadid, Gal, Popovtzer, Rachela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4623670/
https://www.ncbi.nlm.nih.gov/pubmed/26507853
http://dx.doi.org/10.1038/srep15400
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author Betzer, Oshra
Meir, Rinat
Dreifuss, Tamar
Shamalov, Katerina
Motiei, Menachem
Shwartz, Amit
Baranes, Koby
Cohen, Cyrille J.
Shraga-Heled, Niva
Ofir, Racheli
Yadid, Gal
Popovtzer, Rachela
author_facet Betzer, Oshra
Meir, Rinat
Dreifuss, Tamar
Shamalov, Katerina
Motiei, Menachem
Shwartz, Amit
Baranes, Koby
Cohen, Cyrille J.
Shraga-Heled, Niva
Ofir, Racheli
Yadid, Gal
Popovtzer, Rachela
author_sort Betzer, Oshra
collection PubMed
description Recent advances in theranostic nanomedicine can promote stem cell and immune cell-based therapy. Gold nanoparticles (GNPs) have been shown to be promising agents for in-vivo cell-tracking in cell-based therapy applications. Yet a crucial challenge is to develop a reliable protocol for cell upload with, on the one hand, sufficient nanoparticles to achieve maximum visibility of cells, while on the other hand, assuring minimal effect of particles on cell function and viability. Previous studies have demonstrated that the physicochemical parameters of GNPs have a critical impact on their efficient uptake by cells. In the current study we have examined possible variations in GNP uptake, resulting from different incubation period and concentrations in different cell-lines. We have found that GNPs effectively labeled three different cell-lines - stem, immune and cancer cells, with minimal impairment to cell viability and functionality. We further found that uptake efficiency of GNPs into cells stabilized after a short period of time, while GNP concentration had a significant impact on cellular uptake, revealing cell-dependent differences. Our results suggest that while heeding the slight variations within cell lines, modifying the loading time and concentration of GNPs, can promote cell visibility in various nanoparticle-dependent in-vivo cell tracking and imaging applications.
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spelling pubmed-46236702015-11-03 In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications Betzer, Oshra Meir, Rinat Dreifuss, Tamar Shamalov, Katerina Motiei, Menachem Shwartz, Amit Baranes, Koby Cohen, Cyrille J. Shraga-Heled, Niva Ofir, Racheli Yadid, Gal Popovtzer, Rachela Sci Rep Article Recent advances in theranostic nanomedicine can promote stem cell and immune cell-based therapy. Gold nanoparticles (GNPs) have been shown to be promising agents for in-vivo cell-tracking in cell-based therapy applications. Yet a crucial challenge is to develop a reliable protocol for cell upload with, on the one hand, sufficient nanoparticles to achieve maximum visibility of cells, while on the other hand, assuring minimal effect of particles on cell function and viability. Previous studies have demonstrated that the physicochemical parameters of GNPs have a critical impact on their efficient uptake by cells. In the current study we have examined possible variations in GNP uptake, resulting from different incubation period and concentrations in different cell-lines. We have found that GNPs effectively labeled three different cell-lines - stem, immune and cancer cells, with minimal impairment to cell viability and functionality. We further found that uptake efficiency of GNPs into cells stabilized after a short period of time, while GNP concentration had a significant impact on cellular uptake, revealing cell-dependent differences. Our results suggest that while heeding the slight variations within cell lines, modifying the loading time and concentration of GNPs, can promote cell visibility in various nanoparticle-dependent in-vivo cell tracking and imaging applications. Nature Publishing Group 2015-10-28 /pmc/articles/PMC4623670/ /pubmed/26507853 http://dx.doi.org/10.1038/srep15400 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Betzer, Oshra
Meir, Rinat
Dreifuss, Tamar
Shamalov, Katerina
Motiei, Menachem
Shwartz, Amit
Baranes, Koby
Cohen, Cyrille J.
Shraga-Heled, Niva
Ofir, Racheli
Yadid, Gal
Popovtzer, Rachela
In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications
title In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications
title_full In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications
title_fullStr In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications
title_full_unstemmed In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications
title_short In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications
title_sort in-vitro optimization of nanoparticle-cell labeling protocols for in-vivo cell tracking applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4623670/
https://www.ncbi.nlm.nih.gov/pubmed/26507853
http://dx.doi.org/10.1038/srep15400
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