Cargando…

Purification and characterisation of intracellular alpha-galactosidases from Acinetobacter sp.

Two alpha-galactosidases (Ag-I & Ag-II) were purified from Acinetobacter sp. Both the enzymes were monomeric with pH optima of 7.0 and molecular weight of 65 kDa for Ag-I and 37 kDa for Ag-II. The temperature optima for Ag-I was between 50 and 60 °C and that of Ag-II was 40 °C. Both the enzymes...

Descripción completa

Detalles Bibliográficos
Autores principales:	E, Sirisha, Potumarthi, Ravichandra, A, Naveen, Mangamoori, Lakshmi Narasu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2015
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624142/ https://www.ncbi.nlm.nih.gov/pubmed/28324395 http://dx.doi.org/10.1007/s13205-015-0290-9

Ejemplares similares

Role of Feed Forward Neural Networks Coupled with Genetic Algorithm in Capitalizing of Intracellular Alpha-Galactosidase Production by Acinetobacter sp.
por: Edupuganti, Sirisha, et al.
Publicado: (2014)

Constitutive Optimized Production of Streptokinase in Saccharomyces cerevisiae Utilizing Glyceraldehyde 3-Phosphate Dehydrogenase Promoter of Pichia pastoris
por: Vellanki, Ravi N., et al.
Publicado: (2013)

Metagenomic identification, purification and characterisation of the Bifidobacterium adolescentis BgaC β-galactosidase
por: Mulualem, Daniel Mehabie, et al.
Publicado: (2021)

The role of artificial intelligence in tackling COVID-19
por: Arora, Neelima, et al.
Publicado: (2020)

Docking studies of Vitamin C, Vitamin E, Damnacanthal and Scopoletin with human lens gamma D-crystalline
por: Rentala, Satyanarayana, et al.
Publicado: (2013)

Molecular characterization of α-amylase from Staphylococcus aureus
por: Lakshmi, Hanumanthu Prasanna, et al.
Publicado: (2013)

Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
por: Becerra, M, et al.
Publicado: (1998)

Purification and Characterization of Catalase from Marine Bacterium Acinetobacter sp. YS0810
por: Fu, Xinhua, et al.
Publicado: (2014)

A novel cold-active β-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization
por: Wierzbicka-Woś, Anna, et al.
Publicado: (2011)

Overexpression of human alpha-galactosidase A results in its intracellular aggregation, crystallization in lysosomes, and selective secretion
Publicado: (1992)

Functional Characterisation of Alpha-Galactosidase A Mutations as a Basis for a New Classification System in Fabry Disease
por: Lukas, Jan, et al.
Publicado: (2013)

High Throughput Screening for Inhibitors of Alpha-Galactosidase
por: Motabar, Omid, et al.
Publicado: (2010)

Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol
por: Álvarez-Cao, María-Efigenia, et al.
Publicado: (2019)

β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization
por: Eberhardt, María Florencia, et al.
Publicado: (2020)

Cloning, Expression, Purification, and Characterization of β-Galactosidase from Bifidobacterium longum and Bifidobacterium pseudocatenulatum
por: Du, Mingzhu, et al.
Publicado: (2022)

A new cold-adapted β-D-galactosidase from the Antarctic Arthrobacter sp. 32c – gene cloning, overexpression, purification and properties
por: Hildebrandt, Piotr, et al.
Publicado: (2009)

Cloning, expression and characterization of glucokinase gene involved in the glucose-6- phosphate formation in Staphylococcus aureus
por: Lakshmi, Hanumanthu Prasanna, et al.
Publicado: (2013)

Expression, purification and reconstitution of the 4-hydroxybenzoate transporter PcaK from Acinetobacter sp. ADP1()
por: Pernstich, Christian, et al.
Publicado: (2014)

A Proposal for Six Sigma Integration for Large-Scale Production of Penicillin G and Subsequent Conversion to 6-APA
por: Nandi, Anirban, et al.
Publicado: (2014)

Characterisation of a soil MINPP phytase with remarkable long-term stability and activity from Acinetobacter sp.
por: Rix, Gregory D., et al.
Publicado: (2022)

Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi
por: Karan, Ram, et al.
Publicado: (2013)

Intra-Laboratory Validation of Alpha-Galactosidase Activity Measurement in Dietary Supplements
por: Fabris, Elena, et al.
Publicado: (2021)

Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
por: Gururaj, P., et al.
Publicado: (2016)

Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66
por: Shastry, Rajesh Padumane, et al.
Publicado: (2018)

Molecular characterisation of an Acinetobacter baumannii outbreak
por: Al-Hassan, Leena L., et al.
Publicado: (2020)

Production, purification, and process optimization of intracellular pigment from novel psychrotolerant Paenibacillus sp. BPW19
por: Padhan, Bhagyashree, et al.
Publicado: (2021)

Removal of Alpha-Gal Epitopes from Porcine Aortic Valve and Pericardium using Recombinant Human Alpha Galactosidase A
por: Park, Seongsik, et al.
Publicado: (2009)

Enzymatic properties and clinical associations of serum alpha‐galactosidase A in Parkinson's disease
por: Mizutani, Yasuaki, et al.
Publicado: (2023)

Optimization and partial purification of beta-galactosidase production by Aspergillus niger isolated from Brazilian soils using soybean residue
por: Martarello, Raquel Dall’Agnol, et al.
Publicado: (2019)

Purification and Characterisation of Malate Dehydrogenase From Synechocystis sp. PCC 6803: Biochemical Barrier of the Oxidative Tricarboxylic Acid Cycle
por: Takeya, Masahiro, et al.
Publicado: (2018)

Cloning, Expression, Purification, and Characterization of a Novel β-Galactosidase/α-L-Arabinopyranosidase from Paenibacillus polymyxa KF-1
por: Cui, Jing, et al.
Publicado: (2023)

Extracellular α-Galactosidase from Trichoderma sp. (WF-3): Optimization of Enzyme Production and Biochemical Characterization
por: Chauhan, Aishwarya Singh, et al.
Publicado: (2015)

Safety evaluation of the food enzyme beta‐galactosidase from Bacillus sp. (strain M3‐1)
por: Silano, Vittorio, et al.
Publicado: (2019)

Globotriaosylsphingosine Accumulation and Not Alpha-Galactosidase-A Deficiency Causes Endothelial Dysfunction in Fabry Disease
por: Namdar, Mehdi, et al.
Publicado: (2012)

Prediction of the responsiveness to pharmacological chaperones: lysosomal human alpha-galactosidase, a case of study
por: Andreotti, Giuseppina, et al.
Publicado: (2010)

A Novel Frameshift Mutation of Galactosidase-alpha in Fabry Disease Restricted to Dermatologic Manifestations
por: Kim, Dae Hun, et al.
Publicado: (2013)

Affective and cognitive behavior in the alpha-galactosidase A deficient mouse model of Fabry disease
por: Hofmann, Lukas, et al.
Publicado: (2017)

Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii
por: Sikarwar, Juhi, et al.
Publicado: (2013)

Corrigendum: Purification and Characterisation of Malate Dehydrogenase From Synechocystis sp. PCC 6803: Biochemical Barrier of the Oxidative Tricarboxylic Acid Cycle
por: Takeya, Masahiro, et al.
Publicado: (2021)

Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus
por: Negron, Leonardo, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_k4fvq1n2lh7rk6ogp1mb95q5o2