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Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal]

Cross-species transferability and expressed sequence tags (ESTs) in public databases are cost-effective means for developing simple sequence repeats (SSRs) for less-studied species like medicinal plants. In this study, 11 EST–SSR markers developed from 742 available ESTs of Withania Somnifera EST se...

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Autores principales: Parmar, Eva K., Fougat, Ranbir S., Patel, Chandni B., Zala, Harshvardhan N., Patel, Mahesh A., Patel, Swati K., Kumar, Sushil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624151/
https://www.ncbi.nlm.nih.gov/pubmed/28324392
http://dx.doi.org/10.1007/s13205-015-0295-4
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author Parmar, Eva K.
Fougat, Ranbir S.
Patel, Chandni B.
Zala, Harshvardhan N.
Patel, Mahesh A.
Patel, Swati K.
Kumar, Sushil
author_facet Parmar, Eva K.
Fougat, Ranbir S.
Patel, Chandni B.
Zala, Harshvardhan N.
Patel, Mahesh A.
Patel, Swati K.
Kumar, Sushil
author_sort Parmar, Eva K.
collection PubMed
description Cross-species transferability and expressed sequence tags (ESTs) in public databases are cost-effective means for developing simple sequence repeats (SSRs) for less-studied species like medicinal plants. In this study, 11 EST–SSR markers developed from 742 available ESTs of Withania Somnifera EST sequences and 95 SSR primer pairs derived from other solanaceous crops (tomato, eggplant, chili, and tobacco) were utilized for their amplification and validation. Out of 11, 10 EST–SSRs showed good amplification quality and produced 13 loci with a product size ranging between 167 and 291 bp. Similarly, of the 95 cross-genera SSR loci assayed, 20 (21 %) markers showed the transferability of 5, 27, 32, and 14.2 % from eggplant, chili, tomato, and tobacco, respectively, to ashwagandha. In toto, these 30 SSR markers reported here will be valuable resources and may be applicable for the analysis of intra- and inter-specific genetic diversity in ashwagandha for which till date no information about SSR is available.
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spelling pubmed-46241512015-10-30 Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal] Parmar, Eva K. Fougat, Ranbir S. Patel, Chandni B. Zala, Harshvardhan N. Patel, Mahesh A. Patel, Swati K. Kumar, Sushil 3 Biotech Original Article Cross-species transferability and expressed sequence tags (ESTs) in public databases are cost-effective means for developing simple sequence repeats (SSRs) for less-studied species like medicinal plants. In this study, 11 EST–SSR markers developed from 742 available ESTs of Withania Somnifera EST sequences and 95 SSR primer pairs derived from other solanaceous crops (tomato, eggplant, chili, and tobacco) were utilized for their amplification and validation. Out of 11, 10 EST–SSRs showed good amplification quality and produced 13 loci with a product size ranging between 167 and 291 bp. Similarly, of the 95 cross-genera SSR loci assayed, 20 (21 %) markers showed the transferability of 5, 27, 32, and 14.2 % from eggplant, chili, tomato, and tobacco, respectively, to ashwagandha. In toto, these 30 SSR markers reported here will be valuable resources and may be applicable for the analysis of intra- and inter-specific genetic diversity in ashwagandha for which till date no information about SSR is available. Springer Berlin Heidelberg 2015-03-17 2015-12 /pmc/articles/PMC4624151/ /pubmed/28324392 http://dx.doi.org/10.1007/s13205-015-0295-4 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Parmar, Eva K.
Fougat, Ranbir S.
Patel, Chandni B.
Zala, Harshvardhan N.
Patel, Mahesh A.
Patel, Swati K.
Kumar, Sushil
Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal]
title Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal]
title_full Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal]
title_fullStr Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal]
title_full_unstemmed Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal]
title_short Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal]
title_sort validation of dbest-ssrs and transferability of some other solanaceous species ssr in ashwagandha [withania somnifera (l.) dunal]
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624151/
https://www.ncbi.nlm.nih.gov/pubmed/28324392
http://dx.doi.org/10.1007/s13205-015-0295-4
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