Cargando…

Developmental Changes in Composition and Morphology of Cuticular Waxes on Leaves and Spikes of Glossy and Glaucous Wheat (Triticum aestivum L.)

The glossy varieties (A14 and Jing 2001) and glaucous varieties (Fanmai 5 and Shanken 99) of wheat (Triticum aestivum L.) were selected for evaluation of developmental changes in the composition and morphology of cuticular waxes on leaves and spikes. The results provide us with two different wax dev...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yong, Wang, Jiahuan, Chai, Guaiqiang, Li, Chunlian, Hu, Yingang, Chen, Xinhong, Wang, Zhonghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624236/
https://www.ncbi.nlm.nih.gov/pubmed/26506246
http://dx.doi.org/10.1371/journal.pone.0141239
_version_ 1782397795177594880
author Wang, Yong
Wang, Jiahuan
Chai, Guaiqiang
Li, Chunlian
Hu, Yingang
Chen, Xinhong
Wang, Zhonghua
author_facet Wang, Yong
Wang, Jiahuan
Chai, Guaiqiang
Li, Chunlian
Hu, Yingang
Chen, Xinhong
Wang, Zhonghua
author_sort Wang, Yong
collection PubMed
description The glossy varieties (A14 and Jing 2001) and glaucous varieties (Fanmai 5 and Shanken 99) of wheat (Triticum aestivum L.) were selected for evaluation of developmental changes in the composition and morphology of cuticular waxes on leaves and spikes. The results provide us with two different wax development patterns between leaf and spike. The general accumulation trend of the total wax load on leaf and spike surfaces is first to increase and then decrease during the development growth period, but these changes were caused by different compound classes between leaf and spike. Developmental changes of leaf waxes were mainly the result of variations in composition of alcohols and alkanes. In addition, diketones were the third important contributor to the leaf wax changes in the glaucous varieties. Alkanes and diketones were the two major compound classes that caused the developmental changes of spike waxes. For leaf waxes, β- and OH-β-diketones were first detected in flag leaves from 200-day-old plants, and the amounts of β- and OH-β-diketones were significantly higher in glaucous varieties compared with glossy varieties. In spike waxes, β-diketone existed in all varieties, but OH-β-diketone was detectable only in the glaucous varieties. Unexpectedly, the glaucous variety Fanmai 5 yielded large amounts of OH-β-diketone. There was a significant shift in the chain length distribution of alkanes between early stage leaf and flag leaf. Unlike C(28) alcohol being the dominant chain length in leaf waxes, the dominant alcohol chain length of spikes was C(24) or C(26) depending on varieties. Epicuticular wax crystals on wheat leaf and glume were comprised of platelets and tubules, and the crystal morphology changed constantly throughout plant growth, especially the abaxial leaf crystals. Moreover, our results suggested that platelets and tubules on glume surfaces could be formed rapidly within a few days.
format Online
Article
Text
id pubmed-4624236
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-46242362015-11-06 Developmental Changes in Composition and Morphology of Cuticular Waxes on Leaves and Spikes of Glossy and Glaucous Wheat (Triticum aestivum L.) Wang, Yong Wang, Jiahuan Chai, Guaiqiang Li, Chunlian Hu, Yingang Chen, Xinhong Wang, Zhonghua PLoS One Research Article The glossy varieties (A14 and Jing 2001) and glaucous varieties (Fanmai 5 and Shanken 99) of wheat (Triticum aestivum L.) were selected for evaluation of developmental changes in the composition and morphology of cuticular waxes on leaves and spikes. The results provide us with two different wax development patterns between leaf and spike. The general accumulation trend of the total wax load on leaf and spike surfaces is first to increase and then decrease during the development growth period, but these changes were caused by different compound classes between leaf and spike. Developmental changes of leaf waxes were mainly the result of variations in composition of alcohols and alkanes. In addition, diketones were the third important contributor to the leaf wax changes in the glaucous varieties. Alkanes and diketones were the two major compound classes that caused the developmental changes of spike waxes. For leaf waxes, β- and OH-β-diketones were first detected in flag leaves from 200-day-old plants, and the amounts of β- and OH-β-diketones were significantly higher in glaucous varieties compared with glossy varieties. In spike waxes, β-diketone existed in all varieties, but OH-β-diketone was detectable only in the glaucous varieties. Unexpectedly, the glaucous variety Fanmai 5 yielded large amounts of OH-β-diketone. There was a significant shift in the chain length distribution of alkanes between early stage leaf and flag leaf. Unlike C(28) alcohol being the dominant chain length in leaf waxes, the dominant alcohol chain length of spikes was C(24) or C(26) depending on varieties. Epicuticular wax crystals on wheat leaf and glume were comprised of platelets and tubules, and the crystal morphology changed constantly throughout plant growth, especially the abaxial leaf crystals. Moreover, our results suggested that platelets and tubules on glume surfaces could be formed rapidly within a few days. Public Library of Science 2015-10-27 /pmc/articles/PMC4624236/ /pubmed/26506246 http://dx.doi.org/10.1371/journal.pone.0141239 Text en © 2015 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Yong
Wang, Jiahuan
Chai, Guaiqiang
Li, Chunlian
Hu, Yingang
Chen, Xinhong
Wang, Zhonghua
Developmental Changes in Composition and Morphology of Cuticular Waxes on Leaves and Spikes of Glossy and Glaucous Wheat (Triticum aestivum L.)
title Developmental Changes in Composition and Morphology of Cuticular Waxes on Leaves and Spikes of Glossy and Glaucous Wheat (Triticum aestivum L.)
title_full Developmental Changes in Composition and Morphology of Cuticular Waxes on Leaves and Spikes of Glossy and Glaucous Wheat (Triticum aestivum L.)
title_fullStr Developmental Changes in Composition and Morphology of Cuticular Waxes on Leaves and Spikes of Glossy and Glaucous Wheat (Triticum aestivum L.)
title_full_unstemmed Developmental Changes in Composition and Morphology of Cuticular Waxes on Leaves and Spikes of Glossy and Glaucous Wheat (Triticum aestivum L.)
title_short Developmental Changes in Composition and Morphology of Cuticular Waxes on Leaves and Spikes of Glossy and Glaucous Wheat (Triticum aestivum L.)
title_sort developmental changes in composition and morphology of cuticular waxes on leaves and spikes of glossy and glaucous wheat (triticum aestivum l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624236/
https://www.ncbi.nlm.nih.gov/pubmed/26506246
http://dx.doi.org/10.1371/journal.pone.0141239
work_keys_str_mv AT wangyong developmentalchangesincompositionandmorphologyofcuticularwaxesonleavesandspikesofglossyandglaucouswheattriticumaestivuml
AT wangjiahuan developmentalchangesincompositionandmorphologyofcuticularwaxesonleavesandspikesofglossyandglaucouswheattriticumaestivuml
AT chaiguaiqiang developmentalchangesincompositionandmorphologyofcuticularwaxesonleavesandspikesofglossyandglaucouswheattriticumaestivuml
AT lichunlian developmentalchangesincompositionandmorphologyofcuticularwaxesonleavesandspikesofglossyandglaucouswheattriticumaestivuml
AT huyingang developmentalchangesincompositionandmorphologyofcuticularwaxesonleavesandspikesofglossyandglaucouswheattriticumaestivuml
AT chenxinhong developmentalchangesincompositionandmorphologyofcuticularwaxesonleavesandspikesofglossyandglaucouswheattriticumaestivuml
AT wangzhonghua developmentalchangesincompositionandmorphologyofcuticularwaxesonleavesandspikesofglossyandglaucouswheattriticumaestivuml