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EphrinB2 sharpens lateral motor column division in the developing spinal cord
BACKGROUND: During sensori-motor circuit development, the somas of motoneurons (MN) are distributed in a topographic manner in the ventral horn of the neural tube. Indeed, their position within the lateral motor columns (LMC) correlates with axonal trajectories and identity of target limb muscles. T...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624581/ https://www.ncbi.nlm.nih.gov/pubmed/26503288 http://dx.doi.org/10.1186/s13064-015-0051-9 |
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author | Luxey, Maëva Laussu, Julien Davy, Alice |
author_facet | Luxey, Maëva Laussu, Julien Davy, Alice |
author_sort | Luxey, Maëva |
collection | PubMed |
description | BACKGROUND: During sensori-motor circuit development, the somas of motoneurons (MN) are distributed in a topographic manner in the ventral horn of the neural tube. Indeed, their position within the lateral motor columns (LMC) correlates with axonal trajectories and identity of target limb muscles. The mechanisms by which this topographic distribution is established remains poorly understood. To address this issue, we assessed the role of ephrinB2 in MN topographic organization in the developing mouse spinal cord. RESULTS: First, we used a reporter mouse line to establish the spatio-temporal expression pattern of EfnB2 in the developing LMC. We show that early in LMC development, ephrinB2 is differentially expressed in MN of the lateral versus medial LMC, suggesting a possible role in MN sorting and/or migration. We demonstrate that while MN-specific excision of EfnB2 did not perturb specification or migration of MN, conditional loss of ephrinB2 led to the blurring of the LMC divisional boundary and to errors in the selection of LMC axon trajectory in the limb. CONCLUSIONS: Altogether, our study uncovered a novel cell autonomous role for ephrinB2 in LMC MN thus emphasizing the prevalent role of this ephrin member in maintaining cell population boundaries. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13064-015-0051-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4624581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46245812015-10-30 EphrinB2 sharpens lateral motor column division in the developing spinal cord Luxey, Maëva Laussu, Julien Davy, Alice Neural Dev Research Article BACKGROUND: During sensori-motor circuit development, the somas of motoneurons (MN) are distributed in a topographic manner in the ventral horn of the neural tube. Indeed, their position within the lateral motor columns (LMC) correlates with axonal trajectories and identity of target limb muscles. The mechanisms by which this topographic distribution is established remains poorly understood. To address this issue, we assessed the role of ephrinB2 in MN topographic organization in the developing mouse spinal cord. RESULTS: First, we used a reporter mouse line to establish the spatio-temporal expression pattern of EfnB2 in the developing LMC. We show that early in LMC development, ephrinB2 is differentially expressed in MN of the lateral versus medial LMC, suggesting a possible role in MN sorting and/or migration. We demonstrate that while MN-specific excision of EfnB2 did not perturb specification or migration of MN, conditional loss of ephrinB2 led to the blurring of the LMC divisional boundary and to errors in the selection of LMC axon trajectory in the limb. CONCLUSIONS: Altogether, our study uncovered a novel cell autonomous role for ephrinB2 in LMC MN thus emphasizing the prevalent role of this ephrin member in maintaining cell population boundaries. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13064-015-0051-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-26 /pmc/articles/PMC4624581/ /pubmed/26503288 http://dx.doi.org/10.1186/s13064-015-0051-9 Text en © Luxey et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Luxey, Maëva Laussu, Julien Davy, Alice EphrinB2 sharpens lateral motor column division in the developing spinal cord |
title | EphrinB2 sharpens lateral motor column division in the developing spinal cord |
title_full | EphrinB2 sharpens lateral motor column division in the developing spinal cord |
title_fullStr | EphrinB2 sharpens lateral motor column division in the developing spinal cord |
title_full_unstemmed | EphrinB2 sharpens lateral motor column division in the developing spinal cord |
title_short | EphrinB2 sharpens lateral motor column division in the developing spinal cord |
title_sort | ephrinb2 sharpens lateral motor column division in the developing spinal cord |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624581/ https://www.ncbi.nlm.nih.gov/pubmed/26503288 http://dx.doi.org/10.1186/s13064-015-0051-9 |
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