Cargando…

Cromolyn-mediated improvement of intestinal barrier function is associated with enhanced piglet performance after weaning

BACKGROUND: Previous work showed that weaning stress causes gut barrier dysfunction partly by triggering the release of corticotropin releasing factor (CRF) and thereby inducing the degranulation of intestinal mast cell (MC). This study investigated the hypothesis that attenuating the weaning-induce...

Descripción completa

Detalles Bibliográficos
Autores principales: Mereu, Alessandro, Tedó, Gemma, Moeser, Adam J., Rimbach, Gerald, Ipharraguerre, Ignacio R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624645/
https://www.ncbi.nlm.nih.gov/pubmed/26510713
http://dx.doi.org/10.1186/s12917-015-0588-1
_version_ 1782397827987537920
author Mereu, Alessandro
Tedó, Gemma
Moeser, Adam J.
Rimbach, Gerald
Ipharraguerre, Ignacio R.
author_facet Mereu, Alessandro
Tedó, Gemma
Moeser, Adam J.
Rimbach, Gerald
Ipharraguerre, Ignacio R.
author_sort Mereu, Alessandro
collection PubMed
description BACKGROUND: Previous work showed that weaning stress causes gut barrier dysfunction partly by triggering the release of corticotropin releasing factor (CRF) and thereby inducing the degranulation of intestinal mast cell (MC). This study investigated the hypothesis that attenuating the weaning-induced activation of the CRF-MC axis via administration of a MC stabilizing agent (cromolyn) may improve gut permeability and piglet performance after weaning. RESULTS: To test the hypothesis twenty piglets were weaned (20 ± 1.0 d of age; 6.4 ± 0.4 kg of BW) and injected intraperitoneally with saline (control, n = 10) or 20 mg/kg BW of sodium cromolyn (cromolyn, n = 10) at – 0.5, 8 and 16 h relative to weaning. Piglets were housed individually and fed ad libitum a pre-starter diet from one to 15 d post-weaning followed by a starter diet until the end of the study on d 36. Cromolyn improved intestinal permeability as indicated by the reduced recovery of cobalt and mannitol in plasma samples. Cromolyn treated pigs consumed more feed (369 vs. 313 g/d; P < 0.009), gained more BW (283 vs. 238 g/d; P < 0.006), and grew more efficiently (0.60 vs. 0.40; P < 0.042) than their control counterparts. As a result, cromolyn treated pigs were 1.4 kg heavier than those in the control group by d 36 after weaning (16.5 vs. 17.9 kg; P < 0.002). CONCLUSIONS: In agreement with our hypothesis, present data indicate that the cromolyn-mediated improvement of intestinal permeability is associated with enhanced pig performance after weaning.
format Online
Article
Text
id pubmed-4624645
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-46246452015-10-30 Cromolyn-mediated improvement of intestinal barrier function is associated with enhanced piglet performance after weaning Mereu, Alessandro Tedó, Gemma Moeser, Adam J. Rimbach, Gerald Ipharraguerre, Ignacio R. BMC Vet Res Research Article BACKGROUND: Previous work showed that weaning stress causes gut barrier dysfunction partly by triggering the release of corticotropin releasing factor (CRF) and thereby inducing the degranulation of intestinal mast cell (MC). This study investigated the hypothesis that attenuating the weaning-induced activation of the CRF-MC axis via administration of a MC stabilizing agent (cromolyn) may improve gut permeability and piglet performance after weaning. RESULTS: To test the hypothesis twenty piglets were weaned (20 ± 1.0 d of age; 6.4 ± 0.4 kg of BW) and injected intraperitoneally with saline (control, n = 10) or 20 mg/kg BW of sodium cromolyn (cromolyn, n = 10) at – 0.5, 8 and 16 h relative to weaning. Piglets were housed individually and fed ad libitum a pre-starter diet from one to 15 d post-weaning followed by a starter diet until the end of the study on d 36. Cromolyn improved intestinal permeability as indicated by the reduced recovery of cobalt and mannitol in plasma samples. Cromolyn treated pigs consumed more feed (369 vs. 313 g/d; P < 0.009), gained more BW (283 vs. 238 g/d; P < 0.006), and grew more efficiently (0.60 vs. 0.40; P < 0.042) than their control counterparts. As a result, cromolyn treated pigs were 1.4 kg heavier than those in the control group by d 36 after weaning (16.5 vs. 17.9 kg; P < 0.002). CONCLUSIONS: In agreement with our hypothesis, present data indicate that the cromolyn-mediated improvement of intestinal permeability is associated with enhanced pig performance after weaning. BioMed Central 2015-10-28 /pmc/articles/PMC4624645/ /pubmed/26510713 http://dx.doi.org/10.1186/s12917-015-0588-1 Text en © Mereu et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Mereu, Alessandro
Tedó, Gemma
Moeser, Adam J.
Rimbach, Gerald
Ipharraguerre, Ignacio R.
Cromolyn-mediated improvement of intestinal barrier function is associated with enhanced piglet performance after weaning
title Cromolyn-mediated improvement of intestinal barrier function is associated with enhanced piglet performance after weaning
title_full Cromolyn-mediated improvement of intestinal barrier function is associated with enhanced piglet performance after weaning
title_fullStr Cromolyn-mediated improvement of intestinal barrier function is associated with enhanced piglet performance after weaning
title_full_unstemmed Cromolyn-mediated improvement of intestinal barrier function is associated with enhanced piglet performance after weaning
title_short Cromolyn-mediated improvement of intestinal barrier function is associated with enhanced piglet performance after weaning
title_sort cromolyn-mediated improvement of intestinal barrier function is associated with enhanced piglet performance after weaning
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624645/
https://www.ncbi.nlm.nih.gov/pubmed/26510713
http://dx.doi.org/10.1186/s12917-015-0588-1
work_keys_str_mv AT mereualessandro cromolynmediatedimprovementofintestinalbarrierfunctionisassociatedwithenhancedpigletperformanceafterweaning
AT tedogemma cromolynmediatedimprovementofintestinalbarrierfunctionisassociatedwithenhancedpigletperformanceafterweaning
AT moeseradamj cromolynmediatedimprovementofintestinalbarrierfunctionisassociatedwithenhancedpigletperformanceafterweaning
AT rimbachgerald cromolynmediatedimprovementofintestinalbarrierfunctionisassociatedwithenhancedpigletperformanceafterweaning
AT ipharraguerreignacior cromolynmediatedimprovementofintestinalbarrierfunctionisassociatedwithenhancedpigletperformanceafterweaning