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Effects of the Staphylococcus aureus and Staphylococcus epidermidis Secretomes Isolated from the Skin Microbiota of Atopic Children on CD4(+) T Cell Activation

Interactions between the immune system and skin bacteria are of major importance in the pathophysiology of atopic dermatitis (AD), yet our understanding of them is limited. From a cohort of very young AD children (1 to 3 years old), sensitized to Dermatophagoides pteronyssinus allergens (Der p), we...

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Autores principales: Laborel-Préneron, Emeline, Bianchi, Pascale, Boralevi, Franck, Lehours, Philippe, Fraysse, Frédérique, Morice-Picard, Fanny, Sugai, Motoyuki, Sato'o, Yusuke, Badiou, Cédric, Lina, Gérard, Schmitt, Anne-Marie, Redoulès, Daniel, Casas, Christiane, Davrinche, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624846/
https://www.ncbi.nlm.nih.gov/pubmed/26510097
http://dx.doi.org/10.1371/journal.pone.0141067
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author Laborel-Préneron, Emeline
Bianchi, Pascale
Boralevi, Franck
Lehours, Philippe
Fraysse, Frédérique
Morice-Picard, Fanny
Sugai, Motoyuki
Sato'o, Yusuke
Badiou, Cédric
Lina, Gérard
Schmitt, Anne-Marie
Redoulès, Daniel
Casas, Christiane
Davrinche, Christian
author_facet Laborel-Préneron, Emeline
Bianchi, Pascale
Boralevi, Franck
Lehours, Philippe
Fraysse, Frédérique
Morice-Picard, Fanny
Sugai, Motoyuki
Sato'o, Yusuke
Badiou, Cédric
Lina, Gérard
Schmitt, Anne-Marie
Redoulès, Daniel
Casas, Christiane
Davrinche, Christian
author_sort Laborel-Préneron, Emeline
collection PubMed
description Interactions between the immune system and skin bacteria are of major importance in the pathophysiology of atopic dermatitis (AD), yet our understanding of them is limited. From a cohort of very young AD children (1 to 3 years old), sensitized to Dermatophagoides pteronyssinus allergens (Der p), we conducted culturomic analysis of skin microbiota, cutaneous transcript profiling and quantification of anti-Der p CD4(+) T cells. This showed that the presence of S. aureus in inflamed skin of AD patients was associated with a high IgE response, increased expression of inflammatory and Th2/Th22 transcripts and the prevalence of a peripheral Th2 anti-Der p response. Monocyte-derived dendritic cells (moDC) exposed to the S. aureus and S. epidermidis secretomes were found to release pro-inflammatory IFN-γ and anti-inflammatory IL-10, respectively. Allogeneic moDC exposed to the S. aureus secretome also induced the proliferation of CD4(+) T cells and this effect was counteracted by concurrent exposure to the S. epidermidis secretome. In addition, whereas the S. epidermidis secretome promoted the activity of regulatory T cells (Treg) in suppressing the proliferation of conventional CD4(+) T cells, the Treg lost this ability in the presence of the S. aureus secretome. We therefore conclude that S. aureus may cause and promote inflammation in the skin of AD children through concomitant Th2 activation and the silencing of resident Treg cells. Commensals such as S. epidermidis may counteract these effects by inducing the release of IL-10 by skin dendritic cells.
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spelling pubmed-46248462015-11-06 Effects of the Staphylococcus aureus and Staphylococcus epidermidis Secretomes Isolated from the Skin Microbiota of Atopic Children on CD4(+) T Cell Activation Laborel-Préneron, Emeline Bianchi, Pascale Boralevi, Franck Lehours, Philippe Fraysse, Frédérique Morice-Picard, Fanny Sugai, Motoyuki Sato'o, Yusuke Badiou, Cédric Lina, Gérard Schmitt, Anne-Marie Redoulès, Daniel Casas, Christiane Davrinche, Christian PLoS One Research Article Interactions between the immune system and skin bacteria are of major importance in the pathophysiology of atopic dermatitis (AD), yet our understanding of them is limited. From a cohort of very young AD children (1 to 3 years old), sensitized to Dermatophagoides pteronyssinus allergens (Der p), we conducted culturomic analysis of skin microbiota, cutaneous transcript profiling and quantification of anti-Der p CD4(+) T cells. This showed that the presence of S. aureus in inflamed skin of AD patients was associated with a high IgE response, increased expression of inflammatory and Th2/Th22 transcripts and the prevalence of a peripheral Th2 anti-Der p response. Monocyte-derived dendritic cells (moDC) exposed to the S. aureus and S. epidermidis secretomes were found to release pro-inflammatory IFN-γ and anti-inflammatory IL-10, respectively. Allogeneic moDC exposed to the S. aureus secretome also induced the proliferation of CD4(+) T cells and this effect was counteracted by concurrent exposure to the S. epidermidis secretome. In addition, whereas the S. epidermidis secretome promoted the activity of regulatory T cells (Treg) in suppressing the proliferation of conventional CD4(+) T cells, the Treg lost this ability in the presence of the S. aureus secretome. We therefore conclude that S. aureus may cause and promote inflammation in the skin of AD children through concomitant Th2 activation and the silencing of resident Treg cells. Commensals such as S. epidermidis may counteract these effects by inducing the release of IL-10 by skin dendritic cells. Public Library of Science 2015-10-28 /pmc/articles/PMC4624846/ /pubmed/26510097 http://dx.doi.org/10.1371/journal.pone.0141067 Text en © 2015 Laborel-Préneron et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Laborel-Préneron, Emeline
Bianchi, Pascale
Boralevi, Franck
Lehours, Philippe
Fraysse, Frédérique
Morice-Picard, Fanny
Sugai, Motoyuki
Sato'o, Yusuke
Badiou, Cédric
Lina, Gérard
Schmitt, Anne-Marie
Redoulès, Daniel
Casas, Christiane
Davrinche, Christian
Effects of the Staphylococcus aureus and Staphylococcus epidermidis Secretomes Isolated from the Skin Microbiota of Atopic Children on CD4(+) T Cell Activation
title Effects of the Staphylococcus aureus and Staphylococcus epidermidis Secretomes Isolated from the Skin Microbiota of Atopic Children on CD4(+) T Cell Activation
title_full Effects of the Staphylococcus aureus and Staphylococcus epidermidis Secretomes Isolated from the Skin Microbiota of Atopic Children on CD4(+) T Cell Activation
title_fullStr Effects of the Staphylococcus aureus and Staphylococcus epidermidis Secretomes Isolated from the Skin Microbiota of Atopic Children on CD4(+) T Cell Activation
title_full_unstemmed Effects of the Staphylococcus aureus and Staphylococcus epidermidis Secretomes Isolated from the Skin Microbiota of Atopic Children on CD4(+) T Cell Activation
title_short Effects of the Staphylococcus aureus and Staphylococcus epidermidis Secretomes Isolated from the Skin Microbiota of Atopic Children on CD4(+) T Cell Activation
title_sort effects of the staphylococcus aureus and staphylococcus epidermidis secretomes isolated from the skin microbiota of atopic children on cd4(+) t cell activation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624846/
https://www.ncbi.nlm.nih.gov/pubmed/26510097
http://dx.doi.org/10.1371/journal.pone.0141067
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