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Dendritic targeting of short and long 3′ UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins
Sorting of mRNAs in neuronal dendrites relies upon inducible transport mechanisms whose molecular bases are poorly understood. We investigated here the mechanism of inducible dendritic targeting of rat brain-derived neurotrophic factor (BDNF) mRNAs as a paradigmatic example. BDNF encodes multiple mR...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624863/ https://www.ncbi.nlm.nih.gov/pubmed/26578876 http://dx.doi.org/10.3389/fnmol.2015.00062 |
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author | Vicario, Annalisa Colliva, Andrea Ratti, Antonia Davidovic, Laetitia Baj, Gabriele Gricman, Łukasz Colombrita, Claudia Pallavicini, Alberto Jones, Kevin R. Bardoni, Barbara Tongiorgi, Enrico |
author_facet | Vicario, Annalisa Colliva, Andrea Ratti, Antonia Davidovic, Laetitia Baj, Gabriele Gricman, Łukasz Colombrita, Claudia Pallavicini, Alberto Jones, Kevin R. Bardoni, Barbara Tongiorgi, Enrico |
author_sort | Vicario, Annalisa |
collection | PubMed |
description | Sorting of mRNAs in neuronal dendrites relies upon inducible transport mechanisms whose molecular bases are poorly understood. We investigated here the mechanism of inducible dendritic targeting of rat brain-derived neurotrophic factor (BDNF) mRNAs as a paradigmatic example. BDNF encodes multiple mRNAs with either short or long 3′ UTR, both hypothesized to harbor inducible dendritic targeting signals. However, the mechanisms of sorting of the two 3′ UTR isoforms are controversial. We found that dendritic localization of BDNF mRNAs with short 3′ UTR was induced by depolarization and NT3 in vitro or by seizures in vivo and required CPEB-1, -2 and ELAV-2, -4. Dendritic targeting of long 3′ UTR was induced by activity or BDNF and required CPEB-1 and the relief of soma-retention signals mediated by ELAV-1, -3, -4, and FXR proteins. Thus, long and short 3′ UTRs, by using different sets of RNA-binding proteins provide a mechanism of selective targeting in response to different stimuli which may underlay distinct roles of BDNF variants in neuronal development and plasticity. |
format | Online Article Text |
id | pubmed-4624863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46248632015-11-17 Dendritic targeting of short and long 3′ UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins Vicario, Annalisa Colliva, Andrea Ratti, Antonia Davidovic, Laetitia Baj, Gabriele Gricman, Łukasz Colombrita, Claudia Pallavicini, Alberto Jones, Kevin R. Bardoni, Barbara Tongiorgi, Enrico Front Mol Neurosci Neuroscience Sorting of mRNAs in neuronal dendrites relies upon inducible transport mechanisms whose molecular bases are poorly understood. We investigated here the mechanism of inducible dendritic targeting of rat brain-derived neurotrophic factor (BDNF) mRNAs as a paradigmatic example. BDNF encodes multiple mRNAs with either short or long 3′ UTR, both hypothesized to harbor inducible dendritic targeting signals. However, the mechanisms of sorting of the two 3′ UTR isoforms are controversial. We found that dendritic localization of BDNF mRNAs with short 3′ UTR was induced by depolarization and NT3 in vitro or by seizures in vivo and required CPEB-1, -2 and ELAV-2, -4. Dendritic targeting of long 3′ UTR was induced by activity or BDNF and required CPEB-1 and the relief of soma-retention signals mediated by ELAV-1, -3, -4, and FXR proteins. Thus, long and short 3′ UTRs, by using different sets of RNA-binding proteins provide a mechanism of selective targeting in response to different stimuli which may underlay distinct roles of BDNF variants in neuronal development and plasticity. Frontiers Media S.A. 2015-10-29 /pmc/articles/PMC4624863/ /pubmed/26578876 http://dx.doi.org/10.3389/fnmol.2015.00062 Text en Copyright © 2015 Vicario, Colliva, Ratti, Davidovic, Baj, Gricman, Colombrita, Pallavicini, Jones, Bardoni and Tongiorgi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Vicario, Annalisa Colliva, Andrea Ratti, Antonia Davidovic, Laetitia Baj, Gabriele Gricman, Łukasz Colombrita, Claudia Pallavicini, Alberto Jones, Kevin R. Bardoni, Barbara Tongiorgi, Enrico Dendritic targeting of short and long 3′ UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins |
title | Dendritic targeting of short and long 3′ UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins |
title_full | Dendritic targeting of short and long 3′ UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins |
title_fullStr | Dendritic targeting of short and long 3′ UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins |
title_full_unstemmed | Dendritic targeting of short and long 3′ UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins |
title_short | Dendritic targeting of short and long 3′ UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins |
title_sort | dendritic targeting of short and long 3′ utr bdnf mrna is regulated by bdnf or nt-3 and distinct sets of rna-binding proteins |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624863/ https://www.ncbi.nlm.nih.gov/pubmed/26578876 http://dx.doi.org/10.3389/fnmol.2015.00062 |
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