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Smooth Muscle Cells of Penis in the Rat: Noninvasive Quantification with Shear Wave Elastography

Purpose. Smooth muscle cells (SMCs) of cavernosum play an important role in erection. It is of great significance to quantitatively analyze the level of SMCs in penis. In this study, we investigated the feasibility of shear wave elastography (SWE) on evaluating the level of SMCs in penis quantitativ...

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Detalles Bibliográficos
Autores principales: Zhang, Jia-Jie, Qiao, Xiao-Hui, Gao, Feng, Bai, Ming, Li, Fan, Du, Lian-Fang, Xing, Jin-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624877/
https://www.ncbi.nlm.nih.gov/pubmed/26550573
http://dx.doi.org/10.1155/2015/595742
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author Zhang, Jia-Jie
Qiao, Xiao-Hui
Gao, Feng
Bai, Ming
Li, Fan
Du, Lian-Fang
Xing, Jin-Fang
author_facet Zhang, Jia-Jie
Qiao, Xiao-Hui
Gao, Feng
Bai, Ming
Li, Fan
Du, Lian-Fang
Xing, Jin-Fang
author_sort Zhang, Jia-Jie
collection PubMed
description Purpose. Smooth muscle cells (SMCs) of cavernosum play an important role in erection. It is of great significance to quantitatively analyze the level of SMCs in penis. In this study, we investigated the feasibility of shear wave elastography (SWE) on evaluating the level of SMCs in penis quantitatively. Materials and Methods. Twenty healthy male rats were selected. The SWE imaging of penis was carried out and then immunohistochemistry analysis of penis was performed to analyze the expression of alpha smooth muscle actin in penis. The measurement index of SWE examination was tissue stiffness (TS). The measurement index of immunohistochemistry analysis was positive area percentage of alpha smooth muscle actin (AP). Results. Sixty sets of data of TS and AP were obtained. The results showed that TS was significantly correlated with AP and the correlation coefficient was −0.618 (p < 0.001). The result of TS had been plotted against the AP measurements. The relation between the two results has been fitted with quadric curve; the goodness-of-fit index was 0.364 (p < 0.001). Conclusions. The level of SMCs in penis was successfully quantified in vivo with SWE. SWE can be used clinically for evaluating the level of SMCs in penis quantitatively.
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spelling pubmed-46248772015-11-08 Smooth Muscle Cells of Penis in the Rat: Noninvasive Quantification with Shear Wave Elastography Zhang, Jia-Jie Qiao, Xiao-Hui Gao, Feng Bai, Ming Li, Fan Du, Lian-Fang Xing, Jin-Fang Biomed Res Int Research Article Purpose. Smooth muscle cells (SMCs) of cavernosum play an important role in erection. It is of great significance to quantitatively analyze the level of SMCs in penis. In this study, we investigated the feasibility of shear wave elastography (SWE) on evaluating the level of SMCs in penis quantitatively. Materials and Methods. Twenty healthy male rats were selected. The SWE imaging of penis was carried out and then immunohistochemistry analysis of penis was performed to analyze the expression of alpha smooth muscle actin in penis. The measurement index of SWE examination was tissue stiffness (TS). The measurement index of immunohistochemistry analysis was positive area percentage of alpha smooth muscle actin (AP). Results. Sixty sets of data of TS and AP were obtained. The results showed that TS was significantly correlated with AP and the correlation coefficient was −0.618 (p < 0.001). The result of TS had been plotted against the AP measurements. The relation between the two results has been fitted with quadric curve; the goodness-of-fit index was 0.364 (p < 0.001). Conclusions. The level of SMCs in penis was successfully quantified in vivo with SWE. SWE can be used clinically for evaluating the level of SMCs in penis quantitatively. Hindawi Publishing Corporation 2015 2015-10-15 /pmc/articles/PMC4624877/ /pubmed/26550573 http://dx.doi.org/10.1155/2015/595742 Text en Copyright © 2015 Jia-Jie Zhang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Jia-Jie
Qiao, Xiao-Hui
Gao, Feng
Bai, Ming
Li, Fan
Du, Lian-Fang
Xing, Jin-Fang
Smooth Muscle Cells of Penis in the Rat: Noninvasive Quantification with Shear Wave Elastography
title Smooth Muscle Cells of Penis in the Rat: Noninvasive Quantification with Shear Wave Elastography
title_full Smooth Muscle Cells of Penis in the Rat: Noninvasive Quantification with Shear Wave Elastography
title_fullStr Smooth Muscle Cells of Penis in the Rat: Noninvasive Quantification with Shear Wave Elastography
title_full_unstemmed Smooth Muscle Cells of Penis in the Rat: Noninvasive Quantification with Shear Wave Elastography
title_short Smooth Muscle Cells of Penis in the Rat: Noninvasive Quantification with Shear Wave Elastography
title_sort smooth muscle cells of penis in the rat: noninvasive quantification with shear wave elastography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624877/
https://www.ncbi.nlm.nih.gov/pubmed/26550573
http://dx.doi.org/10.1155/2015/595742
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