Cargando…
Emergence of Cryptosporidium hominis Monkey Genotype II and Novel Subtype Family Ik in the Squirrel Monkey (Saimiri sciureus) in China
A single Cryptosporidium isolate from a squirrel monkey with no clinical symptoms was obtained from a zoo in Ya’an city, China, and was genotyped by PCR amplification and DNA sequencing of the small-subunit ribosomal RNA (SSU rRNA), 70-kDa heat shock protein (HSP70), Cryptosporidium oocyst wall prot...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624928/ https://www.ncbi.nlm.nih.gov/pubmed/26509708 http://dx.doi.org/10.1371/journal.pone.0141450 |
_version_ | 1782397892674191360 |
---|---|
author | Liu, Xuehan Xie, Na Li, Wei Zhou, Ziyao Zhong, Zhijun Shen, Liuhong Cao, Suizhong Yu, Xingming Hu, Yanchuan Chen, Weigang Peng, Gangneng |
author_facet | Liu, Xuehan Xie, Na Li, Wei Zhou, Ziyao Zhong, Zhijun Shen, Liuhong Cao, Suizhong Yu, Xingming Hu, Yanchuan Chen, Weigang Peng, Gangneng |
author_sort | Liu, Xuehan |
collection | PubMed |
description | A single Cryptosporidium isolate from a squirrel monkey with no clinical symptoms was obtained from a zoo in Ya’an city, China, and was genotyped by PCR amplification and DNA sequencing of the small-subunit ribosomal RNA (SSU rRNA), 70-kDa heat shock protein (HSP70), Cryptosporidium oocyst wall protein, and actin genes. This multilocus genetic characterization determined that the isolate was Cryptosporidium hominis, but carried 2, 10, and 6 nucleotide differences in the SSU rRNA, HSP70, and actin loci, respectively, which is comparable to the variations at these loci between C. hominis and the previously reported monkey genotype (2, 3, and 3 nucleotide differences). Phylogenetic studies, based on neighbor-joining and maximum likelihood methods, showed that the isolate identified in the current study had a distinctly discordant taxonomic status, distinct from known C. hominis and also from the monkey genotype, with respect to the three loci. Restriction fragment length polymorphisms of the SSU rRNA gene obtained from this study were similar to those of known C. hominis but clearly differentiated from the monkey genotype. Further subtyping was performed by sequence analysis of the gene encoding the 60-kDa glycoprotein (gp60). Maximum homology of only 88.3% to C. hominis subtype IdA10G4 was observed for the current isolate, and phylogenetic analysis demonstrated that this particular isolate belonged to a novel C. hominis subtype family, IkA7G4. This study is the first to report C. hominis infection in the squirrel monkey and, based on the observed genetic characteristics, confirms a new C. hominis genotype, monkey genotype II. Thus, these results provide novel insights into genotypic variation in C. hominis. |
format | Online Article Text |
id | pubmed-4624928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46249282015-11-06 Emergence of Cryptosporidium hominis Monkey Genotype II and Novel Subtype Family Ik in the Squirrel Monkey (Saimiri sciureus) in China Liu, Xuehan Xie, Na Li, Wei Zhou, Ziyao Zhong, Zhijun Shen, Liuhong Cao, Suizhong Yu, Xingming Hu, Yanchuan Chen, Weigang Peng, Gangneng PLoS One Research Article A single Cryptosporidium isolate from a squirrel monkey with no clinical symptoms was obtained from a zoo in Ya’an city, China, and was genotyped by PCR amplification and DNA sequencing of the small-subunit ribosomal RNA (SSU rRNA), 70-kDa heat shock protein (HSP70), Cryptosporidium oocyst wall protein, and actin genes. This multilocus genetic characterization determined that the isolate was Cryptosporidium hominis, but carried 2, 10, and 6 nucleotide differences in the SSU rRNA, HSP70, and actin loci, respectively, which is comparable to the variations at these loci between C. hominis and the previously reported monkey genotype (2, 3, and 3 nucleotide differences). Phylogenetic studies, based on neighbor-joining and maximum likelihood methods, showed that the isolate identified in the current study had a distinctly discordant taxonomic status, distinct from known C. hominis and also from the monkey genotype, with respect to the three loci. Restriction fragment length polymorphisms of the SSU rRNA gene obtained from this study were similar to those of known C. hominis but clearly differentiated from the monkey genotype. Further subtyping was performed by sequence analysis of the gene encoding the 60-kDa glycoprotein (gp60). Maximum homology of only 88.3% to C. hominis subtype IdA10G4 was observed for the current isolate, and phylogenetic analysis demonstrated that this particular isolate belonged to a novel C. hominis subtype family, IkA7G4. This study is the first to report C. hominis infection in the squirrel monkey and, based on the observed genetic characteristics, confirms a new C. hominis genotype, monkey genotype II. Thus, these results provide novel insights into genotypic variation in C. hominis. Public Library of Science 2015-10-28 /pmc/articles/PMC4624928/ /pubmed/26509708 http://dx.doi.org/10.1371/journal.pone.0141450 Text en © 2015 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liu, Xuehan Xie, Na Li, Wei Zhou, Ziyao Zhong, Zhijun Shen, Liuhong Cao, Suizhong Yu, Xingming Hu, Yanchuan Chen, Weigang Peng, Gangneng Emergence of Cryptosporidium hominis Monkey Genotype II and Novel Subtype Family Ik in the Squirrel Monkey (Saimiri sciureus) in China |
title | Emergence of Cryptosporidium hominis Monkey Genotype II and Novel Subtype Family Ik in the Squirrel Monkey (Saimiri sciureus) in China |
title_full | Emergence of Cryptosporidium hominis Monkey Genotype II and Novel Subtype Family Ik in the Squirrel Monkey (Saimiri sciureus) in China |
title_fullStr | Emergence of Cryptosporidium hominis Monkey Genotype II and Novel Subtype Family Ik in the Squirrel Monkey (Saimiri sciureus) in China |
title_full_unstemmed | Emergence of Cryptosporidium hominis Monkey Genotype II and Novel Subtype Family Ik in the Squirrel Monkey (Saimiri sciureus) in China |
title_short | Emergence of Cryptosporidium hominis Monkey Genotype II and Novel Subtype Family Ik in the Squirrel Monkey (Saimiri sciureus) in China |
title_sort | emergence of cryptosporidium hominis monkey genotype ii and novel subtype family ik in the squirrel monkey (saimiri sciureus) in china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624928/ https://www.ncbi.nlm.nih.gov/pubmed/26509708 http://dx.doi.org/10.1371/journal.pone.0141450 |
work_keys_str_mv | AT liuxuehan emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT xiena emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT liwei emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT zhouziyao emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT zhongzhijun emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT shenliuhong emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT caosuizhong emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT yuxingming emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT huyanchuan emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT chenweigang emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina AT penggangneng emergenceofcryptosporidiumhominismonkeygenotypeiiandnovelsubtypefamilyikinthesquirrelmonkeysaimirisciureusinchina |