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Cellular reprogramming through mitogen-activated protein kinases

Mitogen-activated protein kinase (MAPK) cascades are conserved eukaryote signaling modules where MAPKs, as the final kinases in the cascade, phosphorylate protein substrates to regulate cellular processes. While some progress in the identification of MAPK substrates has been made in plants, the know...

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Autores principales: Lee, Justin, Eschen-Lippold, Lennart, Lassowskat, Ines, Böttcher, Christoph, Scheel, Dierk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625042/
https://www.ncbi.nlm.nih.gov/pubmed/26579181
http://dx.doi.org/10.3389/fpls.2015.00940
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author Lee, Justin
Eschen-Lippold, Lennart
Lassowskat, Ines
Böttcher, Christoph
Scheel, Dierk
author_facet Lee, Justin
Eschen-Lippold, Lennart
Lassowskat, Ines
Böttcher, Christoph
Scheel, Dierk
author_sort Lee, Justin
collection PubMed
description Mitogen-activated protein kinase (MAPK) cascades are conserved eukaryote signaling modules where MAPKs, as the final kinases in the cascade, phosphorylate protein substrates to regulate cellular processes. While some progress in the identification of MAPK substrates has been made in plants, the knowledge on the spectrum of substrates and their mechanistic action is still fragmentary. In this focused review, we discuss the biological implications of the data in our original paper (Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana; Frontiers in Plant Science 5: 554) in the context of related research. In our work, we mimicked in vivo activation of two stress-activated MAPKs, MPK3 and MPK6, through transgenic manipulation of Arabidopsis thaliana and used phosphoproteomics analysis to identify potential novel MAPK substrates. Here, we plotted the identified putative MAPK substrates (and downstream phosphoproteins) as a global protein clustering network. Based on a highly stringent selection confidence level, the core networks highlighted a MAPK-induced cellular reprogramming at multiple levels of gene and protein expression—including transcriptional, post-transcriptional, translational, post-translational (such as protein modification, folding, and degradation) steps, and also protein re-compartmentalization. Additionally, the increase in putative substrates/phosphoproteins of energy metabolism and various secondary metabolite biosynthesis pathways coincides with the observed accumulation of defense antimicrobial substances as detected by metabolome analysis. Furthermore, detection of protein networks in phospholipid or redox elements suggests activation of downstream signaling events. Taken in context with other studies, MAPKs are key regulators that reprogram cellular events to orchestrate defense signaling in eukaryotes.
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spelling pubmed-46250422015-11-17 Cellular reprogramming through mitogen-activated protein kinases Lee, Justin Eschen-Lippold, Lennart Lassowskat, Ines Böttcher, Christoph Scheel, Dierk Front Plant Sci Plant Science Mitogen-activated protein kinase (MAPK) cascades are conserved eukaryote signaling modules where MAPKs, as the final kinases in the cascade, phosphorylate protein substrates to regulate cellular processes. While some progress in the identification of MAPK substrates has been made in plants, the knowledge on the spectrum of substrates and their mechanistic action is still fragmentary. In this focused review, we discuss the biological implications of the data in our original paper (Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana; Frontiers in Plant Science 5: 554) in the context of related research. In our work, we mimicked in vivo activation of two stress-activated MAPKs, MPK3 and MPK6, through transgenic manipulation of Arabidopsis thaliana and used phosphoproteomics analysis to identify potential novel MAPK substrates. Here, we plotted the identified putative MAPK substrates (and downstream phosphoproteins) as a global protein clustering network. Based on a highly stringent selection confidence level, the core networks highlighted a MAPK-induced cellular reprogramming at multiple levels of gene and protein expression—including transcriptional, post-transcriptional, translational, post-translational (such as protein modification, folding, and degradation) steps, and also protein re-compartmentalization. Additionally, the increase in putative substrates/phosphoproteins of energy metabolism and various secondary metabolite biosynthesis pathways coincides with the observed accumulation of defense antimicrobial substances as detected by metabolome analysis. Furthermore, detection of protein networks in phospholipid or redox elements suggests activation of downstream signaling events. Taken in context with other studies, MAPKs are key regulators that reprogram cellular events to orchestrate defense signaling in eukaryotes. Frontiers Media S.A. 2015-10-29 /pmc/articles/PMC4625042/ /pubmed/26579181 http://dx.doi.org/10.3389/fpls.2015.00940 Text en Copyright © 2015 Lee, Eschen-Lippold, Lassowskat, Böttcher and Scheel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lee, Justin
Eschen-Lippold, Lennart
Lassowskat, Ines
Böttcher, Christoph
Scheel, Dierk
Cellular reprogramming through mitogen-activated protein kinases
title Cellular reprogramming through mitogen-activated protein kinases
title_full Cellular reprogramming through mitogen-activated protein kinases
title_fullStr Cellular reprogramming through mitogen-activated protein kinases
title_full_unstemmed Cellular reprogramming through mitogen-activated protein kinases
title_short Cellular reprogramming through mitogen-activated protein kinases
title_sort cellular reprogramming through mitogen-activated protein kinases
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625042/
https://www.ncbi.nlm.nih.gov/pubmed/26579181
http://dx.doi.org/10.3389/fpls.2015.00940
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