Cargando…

Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury

Hypoxic preconditioning activates endogenous mechanisms that protect against cerebral ischemic and hypoxic injury. To better understand these protective mechanisms, adult rats were housed in a hypoxic environment (8% O(2)/92% N(2)) for 3 hours, and then in a normal oxygen environment for 12 hours. T...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yan-bo, Guo, Zheng-dong, Li, Mei-yi, Li, Si-jie, Niu, Jing-zhong, Yang, Ming-feng, Ji, Xun-ming, Lv, Guo-wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625514/
https://www.ncbi.nlm.nih.gov/pubmed/26604909
http://dx.doi.org/10.4103/1673-5374.165519
_version_ 1782397992401108992
author Zhang, Yan-bo
Guo, Zheng-dong
Li, Mei-yi
Li, Si-jie
Niu, Jing-zhong
Yang, Ming-feng
Ji, Xun-ming
Lv, Guo-wei
author_facet Zhang, Yan-bo
Guo, Zheng-dong
Li, Mei-yi
Li, Si-jie
Niu, Jing-zhong
Yang, Ming-feng
Ji, Xun-ming
Lv, Guo-wei
author_sort Zhang, Yan-bo
collection PubMed
description Hypoxic preconditioning activates endogenous mechanisms that protect against cerebral ischemic and hypoxic injury. To better understand these protective mechanisms, adult rats were housed in a hypoxic environment (8% O(2)/92% N(2)) for 3 hours, and then in a normal oxygen environment for 12 hours. Their cerebrospinal fluid was obtained to culture cortical neurons from newborn rats for 1 day, and then the neurons were exposed to oxygen-glucose deprivation for 1.5 hours. The cerebrospinal fluid from rats subjected to hypoxic preconditioning reduced oxygen-glucose deprivation-induced injury, increased survival rate, upregulated Bcl-2 expression and downregulated Bax expression in the cultured cortical neurons, compared with control. These results indicate that cerebrospinal fluid from rats given hypoxic preconditioning protects against oxygen-glucose deprivation-induced injury by affecting apoptosis-related protein expression in neurons from newborn rats.
format Online
Article
Text
id pubmed-4625514
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-46255142015-11-24 Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury Zhang, Yan-bo Guo, Zheng-dong Li, Mei-yi Li, Si-jie Niu, Jing-zhong Yang, Ming-feng Ji, Xun-ming Lv, Guo-wei Neural Regen Res Research Article Hypoxic preconditioning activates endogenous mechanisms that protect against cerebral ischemic and hypoxic injury. To better understand these protective mechanisms, adult rats were housed in a hypoxic environment (8% O(2)/92% N(2)) for 3 hours, and then in a normal oxygen environment for 12 hours. Their cerebrospinal fluid was obtained to culture cortical neurons from newborn rats for 1 day, and then the neurons were exposed to oxygen-glucose deprivation for 1.5 hours. The cerebrospinal fluid from rats subjected to hypoxic preconditioning reduced oxygen-glucose deprivation-induced injury, increased survival rate, upregulated Bcl-2 expression and downregulated Bax expression in the cultured cortical neurons, compared with control. These results indicate that cerebrospinal fluid from rats given hypoxic preconditioning protects against oxygen-glucose deprivation-induced injury by affecting apoptosis-related protein expression in neurons from newborn rats. Medknow Publications & Media Pvt Ltd 2015-09 /pmc/articles/PMC4625514/ /pubmed/26604909 http://dx.doi.org/10.4103/1673-5374.165519 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Zhang, Yan-bo
Guo, Zheng-dong
Li, Mei-yi
Li, Si-jie
Niu, Jing-zhong
Yang, Ming-feng
Ji, Xun-ming
Lv, Guo-wei
Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury
title Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury
title_full Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury
title_fullStr Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury
title_full_unstemmed Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury
title_short Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury
title_sort cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625514/
https://www.ncbi.nlm.nih.gov/pubmed/26604909
http://dx.doi.org/10.4103/1673-5374.165519
work_keys_str_mv AT zhangyanbo cerebrospinalfluidfromratsgivenhypoxicpreconditioningprotectsneuronsfromoxygenglucosedeprivationinducedinjury
AT guozhengdong cerebrospinalfluidfromratsgivenhypoxicpreconditioningprotectsneuronsfromoxygenglucosedeprivationinducedinjury
AT limeiyi cerebrospinalfluidfromratsgivenhypoxicpreconditioningprotectsneuronsfromoxygenglucosedeprivationinducedinjury
AT lisijie cerebrospinalfluidfromratsgivenhypoxicpreconditioningprotectsneuronsfromoxygenglucosedeprivationinducedinjury
AT niujingzhong cerebrospinalfluidfromratsgivenhypoxicpreconditioningprotectsneuronsfromoxygenglucosedeprivationinducedinjury
AT yangmingfeng cerebrospinalfluidfromratsgivenhypoxicpreconditioningprotectsneuronsfromoxygenglucosedeprivationinducedinjury
AT jixunming cerebrospinalfluidfromratsgivenhypoxicpreconditioningprotectsneuronsfromoxygenglucosedeprivationinducedinjury
AT lvguowei cerebrospinalfluidfromratsgivenhypoxicpreconditioningprotectsneuronsfromoxygenglucosedeprivationinducedinjury