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Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control

BACKGROUND: Introduced Wolbachia bacteria can influence the susceptibility of Aedes aegypti mosquitoes to arboviral infections as well as having detrimental effects on host fitness. Previous field trials demonstrated that the wMel strain of Wolbachia effectively and durably invades Ae. aegypti popul...

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Autores principales: Nguyen, Tran Hien, Nguyen, H. Le, Nguyen, Thu Yen, Vu, Sinh Nam, Tran, Nhu Duong, Le, T. N., Vien, Quang Mai, Bui, T. C., Le, Huu Tho, Kutcher, Simon, Hurst, Tim P., Duong, T. T. H., Jeffery, Jason A. L., Darbro, Jonathan M., Kay, B. H., Iturbe-Ormaetxe, Iñaki, Popovici, Jean, Montgomery, Brian L., Turley, Andrew P., Zigterman, Flora, Cook, Helen, Cook, Peter E., Johnson, Petrina H., Ryan, Peter A., Paton, Chris J., Ritchie, Scott A., Simmons, Cameron P., O’Neill, Scott L., Hoffmann, Ary A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625535/
https://www.ncbi.nlm.nih.gov/pubmed/26510523
http://dx.doi.org/10.1186/s13071-015-1174-x
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author Nguyen, Tran Hien
Nguyen, H. Le
Nguyen, Thu Yen
Vu, Sinh Nam
Tran, Nhu Duong
Le, T. N.
Vien, Quang Mai
Bui, T. C.
Le, Huu Tho
Kutcher, Simon
Hurst, Tim P.
Duong, T. T. H.
Jeffery, Jason A. L.
Darbro, Jonathan M.
Kay, B. H.
Iturbe-Ormaetxe, Iñaki
Popovici, Jean
Montgomery, Brian L.
Turley, Andrew P.
Zigterman, Flora
Cook, Helen
Cook, Peter E.
Johnson, Petrina H.
Ryan, Peter A.
Paton, Chris J.
Ritchie, Scott A.
Simmons, Cameron P.
O’Neill, Scott L.
Hoffmann, Ary A.
author_facet Nguyen, Tran Hien
Nguyen, H. Le
Nguyen, Thu Yen
Vu, Sinh Nam
Tran, Nhu Duong
Le, T. N.
Vien, Quang Mai
Bui, T. C.
Le, Huu Tho
Kutcher, Simon
Hurst, Tim P.
Duong, T. T. H.
Jeffery, Jason A. L.
Darbro, Jonathan M.
Kay, B. H.
Iturbe-Ormaetxe, Iñaki
Popovici, Jean
Montgomery, Brian L.
Turley, Andrew P.
Zigterman, Flora
Cook, Helen
Cook, Peter E.
Johnson, Petrina H.
Ryan, Peter A.
Paton, Chris J.
Ritchie, Scott A.
Simmons, Cameron P.
O’Neill, Scott L.
Hoffmann, Ary A.
author_sort Nguyen, Tran Hien
collection PubMed
description BACKGROUND: Introduced Wolbachia bacteria can influence the susceptibility of Aedes aegypti mosquitoes to arboviral infections as well as having detrimental effects on host fitness. Previous field trials demonstrated that the wMel strain of Wolbachia effectively and durably invades Ae. aegypti populations. Here we report on trials of a second strain, wMelPop-PGYP Wolbachia, in field sites in northern Australia (Machans Beach and Babinda) and central Vietnam (Tri Nguyen, Hon Mieu Island), each with contrasting natural Ae. aegypti densities. METHODS: Mosquitoes were released at the adult or pupal stages for different lengths of time at the sites depending on changes in Wolbachia frequency as assessed through PCR assays of material collected through Biogents-Sentinel (BG-S) traps and ovitraps. Adult numbers were also monitored through BG-S traps. Changes in Wolbachia frequency were compared across hamlets or house blocks. RESULTS: Releases of adult wMelPop-Ae. aegypti resulted in the transient invasion of wMelPop in all three field sites. Invasion at the Australian sites was heterogeneous, reflecting a slower rate of invasion in locations where background mosquito numbers were high. In contrast, invasion across Tri Nguyen was relatively uniform. After cessation of releases, the frequency of wMelPop declined in all sites, most rapidly in Babinda and Tri Nguyen. Within Machans Beach the rate of decrease varied among areas, and wMelPop was detected for several months in an area with a relatively low mosquito density. CONCLUSIONS: These findings highlight challenges associated with releasing Wolbachia-Ae. aegypti combinations with low fitness, albeit strong virus interference properties, as a means of sustainable control of dengue virus transmission. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-1174-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-46255352015-10-30 Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control Nguyen, Tran Hien Nguyen, H. Le Nguyen, Thu Yen Vu, Sinh Nam Tran, Nhu Duong Le, T. N. Vien, Quang Mai Bui, T. C. Le, Huu Tho Kutcher, Simon Hurst, Tim P. Duong, T. T. H. Jeffery, Jason A. L. Darbro, Jonathan M. Kay, B. H. Iturbe-Ormaetxe, Iñaki Popovici, Jean Montgomery, Brian L. Turley, Andrew P. Zigterman, Flora Cook, Helen Cook, Peter E. Johnson, Petrina H. Ryan, Peter A. Paton, Chris J. Ritchie, Scott A. Simmons, Cameron P. O’Neill, Scott L. Hoffmann, Ary A. Parasit Vectors Research BACKGROUND: Introduced Wolbachia bacteria can influence the susceptibility of Aedes aegypti mosquitoes to arboviral infections as well as having detrimental effects on host fitness. Previous field trials demonstrated that the wMel strain of Wolbachia effectively and durably invades Ae. aegypti populations. Here we report on trials of a second strain, wMelPop-PGYP Wolbachia, in field sites in northern Australia (Machans Beach and Babinda) and central Vietnam (Tri Nguyen, Hon Mieu Island), each with contrasting natural Ae. aegypti densities. METHODS: Mosquitoes were released at the adult or pupal stages for different lengths of time at the sites depending on changes in Wolbachia frequency as assessed through PCR assays of material collected through Biogents-Sentinel (BG-S) traps and ovitraps. Adult numbers were also monitored through BG-S traps. Changes in Wolbachia frequency were compared across hamlets or house blocks. RESULTS: Releases of adult wMelPop-Ae. aegypti resulted in the transient invasion of wMelPop in all three field sites. Invasion at the Australian sites was heterogeneous, reflecting a slower rate of invasion in locations where background mosquito numbers were high. In contrast, invasion across Tri Nguyen was relatively uniform. After cessation of releases, the frequency of wMelPop declined in all sites, most rapidly in Babinda and Tri Nguyen. Within Machans Beach the rate of decrease varied among areas, and wMelPop was detected for several months in an area with a relatively low mosquito density. CONCLUSIONS: These findings highlight challenges associated with releasing Wolbachia-Ae. aegypti combinations with low fitness, albeit strong virus interference properties, as a means of sustainable control of dengue virus transmission. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-1174-x) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-28 /pmc/articles/PMC4625535/ /pubmed/26510523 http://dx.doi.org/10.1186/s13071-015-1174-x Text en © Nguyen et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Nguyen, Tran Hien
Nguyen, H. Le
Nguyen, Thu Yen
Vu, Sinh Nam
Tran, Nhu Duong
Le, T. N.
Vien, Quang Mai
Bui, T. C.
Le, Huu Tho
Kutcher, Simon
Hurst, Tim P.
Duong, T. T. H.
Jeffery, Jason A. L.
Darbro, Jonathan M.
Kay, B. H.
Iturbe-Ormaetxe, Iñaki
Popovici, Jean
Montgomery, Brian L.
Turley, Andrew P.
Zigterman, Flora
Cook, Helen
Cook, Peter E.
Johnson, Petrina H.
Ryan, Peter A.
Paton, Chris J.
Ritchie, Scott A.
Simmons, Cameron P.
O’Neill, Scott L.
Hoffmann, Ary A.
Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control
title Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control
title_full Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control
title_fullStr Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control
title_full_unstemmed Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control
title_short Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control
title_sort field evaluation of the establishment potential of wmelpop wolbachia in australia and vietnam for dengue control
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625535/
https://www.ncbi.nlm.nih.gov/pubmed/26510523
http://dx.doi.org/10.1186/s13071-015-1174-x
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