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Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease

ABSTRACT: Many proteins of viruses infecting hyperthermophilic Crenarchaeota have no detectable homologs in current databases, hampering our understanding of viral evolution. We used sensitive database search methods and structural modeling to show that a nucleocapsid protein (TP1) of Thermoproteus...

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Autores principales: Krupovic, Mart, Cvirkaite-Krupovic, Virginija, Prangishvili, David, Koonin, Eugene V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625639/
https://www.ncbi.nlm.nih.gov/pubmed/26514828
http://dx.doi.org/10.1186/s13062-015-0093-2
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author Krupovic, Mart
Cvirkaite-Krupovic, Virginija
Prangishvili, David
Koonin, Eugene V.
author_facet Krupovic, Mart
Cvirkaite-Krupovic, Virginija
Prangishvili, David
Koonin, Eugene V.
author_sort Krupovic, Mart
collection PubMed
description ABSTRACT: Many proteins of viruses infecting hyperthermophilic Crenarchaeota have no detectable homologs in current databases, hampering our understanding of viral evolution. We used sensitive database search methods and structural modeling to show that a nucleocapsid protein (TP1) of Thermoproteus tenax virus 1 (TTV1) is a derivative of the Cas4 nuclease, a component of the CRISPR-Cas adaptive immunity system that is encoded also by several archaeal viruses. In TTV1, the Cas4 gene was split into two, with the N-terminal portion becoming TP1, and lost some of the catalytic amino acid residues, apparently resulting in the inactivation of the nuclease. To our knowledge, this is the first described case of exaptation of an enzyme for a virus capsid protein function. REVIEWERS: This article was reviewed by Vivek Anantharaman, Christine Orengo and Mircea Podar. For complete reviews, see the Reviewers’ reports section. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13062-015-0093-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-46256392015-10-30 Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease Krupovic, Mart Cvirkaite-Krupovic, Virginija Prangishvili, David Koonin, Eugene V. Biol Direct Discovery Notes ABSTRACT: Many proteins of viruses infecting hyperthermophilic Crenarchaeota have no detectable homologs in current databases, hampering our understanding of viral evolution. We used sensitive database search methods and structural modeling to show that a nucleocapsid protein (TP1) of Thermoproteus tenax virus 1 (TTV1) is a derivative of the Cas4 nuclease, a component of the CRISPR-Cas adaptive immunity system that is encoded also by several archaeal viruses. In TTV1, the Cas4 gene was split into two, with the N-terminal portion becoming TP1, and lost some of the catalytic amino acid residues, apparently resulting in the inactivation of the nuclease. To our knowledge, this is the first described case of exaptation of an enzyme for a virus capsid protein function. REVIEWERS: This article was reviewed by Vivek Anantharaman, Christine Orengo and Mircea Podar. For complete reviews, see the Reviewers’ reports section. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13062-015-0093-2) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-29 /pmc/articles/PMC4625639/ /pubmed/26514828 http://dx.doi.org/10.1186/s13062-015-0093-2 Text en © Krupovic et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Discovery Notes
Krupovic, Mart
Cvirkaite-Krupovic, Virginija
Prangishvili, David
Koonin, Eugene V.
Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease
title Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease
title_full Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease
title_fullStr Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease
title_full_unstemmed Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease
title_short Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease
title_sort evolution of an archaeal virus nucleocapsid protein from the crispr-associated cas4 nuclease
topic Discovery Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625639/
https://www.ncbi.nlm.nih.gov/pubmed/26514828
http://dx.doi.org/10.1186/s13062-015-0093-2
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