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Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources

BACKGROUND: Lactobacillus plantarum is a lactic acid bacterium (LAB) of considerable industrial interest since it has an important role in the production of fermented food. In the present study, the genetic diversity and population structure within 186 L. plantarum isolates was determined based on a...

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Autores principales: Xu, Haiyan, Liu, Wenjun, Zhang, Wenyi, Yu, Jie, Song, Yuqin, Menhe, Bilige, Zhang, Heping, Sun, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625847/
https://www.ncbi.nlm.nih.gov/pubmed/26511725
http://dx.doi.org/10.1186/s12866-015-0584-4
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author Xu, Haiyan
Liu, Wenjun
Zhang, Wenyi
Yu, Jie
Song, Yuqin
Menhe, Bilige
Zhang, Heping
Sun, Zhihong
author_facet Xu, Haiyan
Liu, Wenjun
Zhang, Wenyi
Yu, Jie
Song, Yuqin
Menhe, Bilige
Zhang, Heping
Sun, Zhihong
author_sort Xu, Haiyan
collection PubMed
description BACKGROUND: Lactobacillus plantarum is a lactic acid bacterium (LAB) of considerable industrial interest since it has an important role in the production of fermented food. In the present study, the genetic diversity and population structure within 186 L. plantarum isolates was determined based on a novel MLST scheme employing eight housekeeping genes. These isolates had originated from different sources and geographic regions: 179 isolates were from our own culture collection and originated from China and Mongolia and seven isolates were type or reference isolates from other collections. RESULTS: The results showed that 179 isolates and seven reference isolates could be assigned to 73 different sequence types (STs), forming ten clonal complexes (CCs) and 23 singletons. There were 158 polymorphic sites detected in total, and the nucleotide diversity per site varied from 0.00401 in clpX to 0.03220 in groEL. The minimum spanning tree analyses suggested that the evolution of L. plantarum isolates have little relationship with ecological sources have similar nucleotide diversity. Phylogenetic trees and structure indicated that there were six lineages in the L. plantarum isolates used in our study. Split-decomposition and ClonalFrame analysis indicated that recombination had occurred throughout the population of L. plantarum, but it occurred at a low frequency in these eight loci. CONCLUSION: We deduced that L. plantarum isolates from the same ecological niches have similar genetic diversity and population structure. The MLST scheme presented in this study provides abundant sequence data for L. plantarum and enabled global comparisons of isolates associated with various environmental origins to be made. This will further advance our understanding of the microbial ecology of this industrially important LAB. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-015-0584-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-46258472015-10-30 Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources Xu, Haiyan Liu, Wenjun Zhang, Wenyi Yu, Jie Song, Yuqin Menhe, Bilige Zhang, Heping Sun, Zhihong BMC Microbiol Research Article BACKGROUND: Lactobacillus plantarum is a lactic acid bacterium (LAB) of considerable industrial interest since it has an important role in the production of fermented food. In the present study, the genetic diversity and population structure within 186 L. plantarum isolates was determined based on a novel MLST scheme employing eight housekeeping genes. These isolates had originated from different sources and geographic regions: 179 isolates were from our own culture collection and originated from China and Mongolia and seven isolates were type or reference isolates from other collections. RESULTS: The results showed that 179 isolates and seven reference isolates could be assigned to 73 different sequence types (STs), forming ten clonal complexes (CCs) and 23 singletons. There were 158 polymorphic sites detected in total, and the nucleotide diversity per site varied from 0.00401 in clpX to 0.03220 in groEL. The minimum spanning tree analyses suggested that the evolution of L. plantarum isolates have little relationship with ecological sources have similar nucleotide diversity. Phylogenetic trees and structure indicated that there were six lineages in the L. plantarum isolates used in our study. Split-decomposition and ClonalFrame analysis indicated that recombination had occurred throughout the population of L. plantarum, but it occurred at a low frequency in these eight loci. CONCLUSION: We deduced that L. plantarum isolates from the same ecological niches have similar genetic diversity and population structure. The MLST scheme presented in this study provides abundant sequence data for L. plantarum and enabled global comparisons of isolates associated with various environmental origins to be made. This will further advance our understanding of the microbial ecology of this industrially important LAB. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-015-0584-4) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-28 /pmc/articles/PMC4625847/ /pubmed/26511725 http://dx.doi.org/10.1186/s12866-015-0584-4 Text en © Xu et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Xu, Haiyan
Liu, Wenjun
Zhang, Wenyi
Yu, Jie
Song, Yuqin
Menhe, Bilige
Zhang, Heping
Sun, Zhihong
Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources
title Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources
title_full Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources
title_fullStr Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources
title_full_unstemmed Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources
title_short Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources
title_sort use of multilocus sequence typing to infer genetic diversity and population structure of lactobacillus plantarum isolates from different sources
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625847/
https://www.ncbi.nlm.nih.gov/pubmed/26511725
http://dx.doi.org/10.1186/s12866-015-0584-4
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