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Modulation of dendritic cell alternative activation and function by the vitamin A metabolite retinoic acid

The archetypal T(h)2 cytokine IL-4 has previously been shown to alternatively activate murine macrophages and, more recently, dendritic cells (DCs) both in vitro and in vivo. IL-4 has also been shown to induce Aldh1a2 (aldehyde dehydrogenase 1a2) expression in murine macrophages recruited to the per...

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Autores principales: Jones, Lucy H., Cook, Peter C., Ivens, Alasdair C., Thomas, Graham, D., Phythian-Adams, Alexander T., Allen, Judith E., MacDonald, Andrew S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625886/
https://www.ncbi.nlm.nih.gov/pubmed/25899567
http://dx.doi.org/10.1093/intimm/dxv020
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author Jones, Lucy H.
Cook, Peter C.
Ivens, Alasdair C.
Thomas, Graham, D.
Phythian-Adams, Alexander T.
Allen, Judith E.
MacDonald, Andrew S.
author_facet Jones, Lucy H.
Cook, Peter C.
Ivens, Alasdair C.
Thomas, Graham, D.
Phythian-Adams, Alexander T.
Allen, Judith E.
MacDonald, Andrew S.
author_sort Jones, Lucy H.
collection PubMed
description The archetypal T(h)2 cytokine IL-4 has previously been shown to alternatively activate murine macrophages and, more recently, dendritic cells (DCs) both in vitro and in vivo. IL-4 has also been shown to induce Aldh1a2 (aldehyde dehydrogenase 1a2) expression in murine macrophages recruited to the peritoneal cavity. However, the influence of IL-4 on DC Aldh1a2 induction in vivo has not yet been addressed. In this work, we found that DCs show enhanced aldehyde dehydrogenase enzyme activity in vivo, which led us to investigate the impact of the vitamin A metabolite all-trans retinoic acid (RA) on DC alternative activation and function. Antagonism of RA receptors reduced production of resistin-like molecule alpha by DCs responding to IL-4, while addition of exogenous RA enhanced production of this marker of alternative activation. Functionally, RA increased DC induction of CD4(+) T-cell IL-10, while reducing CD4(+) T-cell IL-4 and IL-13, revealing a previously unidentified role for RA in regulating the ability of alternatively activated DCs to influence T(h)2 polarization.
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spelling pubmed-46258862015-10-30 Modulation of dendritic cell alternative activation and function by the vitamin A metabolite retinoic acid Jones, Lucy H. Cook, Peter C. Ivens, Alasdair C. Thomas, Graham, D. Phythian-Adams, Alexander T. Allen, Judith E. MacDonald, Andrew S. Int Immunol Featured Article of the Month The archetypal T(h)2 cytokine IL-4 has previously been shown to alternatively activate murine macrophages and, more recently, dendritic cells (DCs) both in vitro and in vivo. IL-4 has also been shown to induce Aldh1a2 (aldehyde dehydrogenase 1a2) expression in murine macrophages recruited to the peritoneal cavity. However, the influence of IL-4 on DC Aldh1a2 induction in vivo has not yet been addressed. In this work, we found that DCs show enhanced aldehyde dehydrogenase enzyme activity in vivo, which led us to investigate the impact of the vitamin A metabolite all-trans retinoic acid (RA) on DC alternative activation and function. Antagonism of RA receptors reduced production of resistin-like molecule alpha by DCs responding to IL-4, while addition of exogenous RA enhanced production of this marker of alternative activation. Functionally, RA increased DC induction of CD4(+) T-cell IL-10, while reducing CD4(+) T-cell IL-4 and IL-13, revealing a previously unidentified role for RA in regulating the ability of alternatively activated DCs to influence T(h)2 polarization. Oxford University Press 2015-11 2015-04-20 /pmc/articles/PMC4625886/ /pubmed/25899567 http://dx.doi.org/10.1093/intimm/dxv020 Text en © The Author 2015. Published by Oxford University Press on behalf of The Japanese Society for Immunology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Featured Article of the Month
Jones, Lucy H.
Cook, Peter C.
Ivens, Alasdair C.
Thomas, Graham, D.
Phythian-Adams, Alexander T.
Allen, Judith E.
MacDonald, Andrew S.
Modulation of dendritic cell alternative activation and function by the vitamin A metabolite retinoic acid
title Modulation of dendritic cell alternative activation and function by the vitamin A metabolite retinoic acid
title_full Modulation of dendritic cell alternative activation and function by the vitamin A metabolite retinoic acid
title_fullStr Modulation of dendritic cell alternative activation and function by the vitamin A metabolite retinoic acid
title_full_unstemmed Modulation of dendritic cell alternative activation and function by the vitamin A metabolite retinoic acid
title_short Modulation of dendritic cell alternative activation and function by the vitamin A metabolite retinoic acid
title_sort modulation of dendritic cell alternative activation and function by the vitamin a metabolite retinoic acid
topic Featured Article of the Month
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625886/
https://www.ncbi.nlm.nih.gov/pubmed/25899567
http://dx.doi.org/10.1093/intimm/dxv020
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