Negative studies are helpful to compute the specificity of diagnostic tests: measuring Trypanosoma cruzi seroprevalence in Guanajuato, Mexico

BACKGROUND: Publishing negative seroprevalence studies not only helps to have more accurate seroprevalence estimates but also allows calculating the specificity of the diagnostic tests used. We performed a population-based Trypanosoma cruzi seroprevalence survey in a community in central Mexico. RES...

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Autores principales: Padilla-Raygoza, Nicolás, Gamboa-León, Rubí, Ramirez-Sierra, Maria Jesus, Dumonteil, Eric, Buekens, Pierre, Ruiz-Paloalto, Ma Laura, Diaz-Guerrero, Rosalina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625936/
https://www.ncbi.nlm.nih.gov/pubmed/26510987
http://dx.doi.org/10.1186/s13104-015-1612-z
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author Padilla-Raygoza, Nicolás
Gamboa-León, Rubí
Ramirez-Sierra, Maria Jesus
Dumonteil, Eric
Buekens, Pierre
Ruiz-Paloalto, Ma Laura
Diaz-Guerrero, Rosalina
author_facet Padilla-Raygoza, Nicolás
Gamboa-León, Rubí
Ramirez-Sierra, Maria Jesus
Dumonteil, Eric
Buekens, Pierre
Ruiz-Paloalto, Ma Laura
Diaz-Guerrero, Rosalina
author_sort Padilla-Raygoza, Nicolás
collection PubMed
description BACKGROUND: Publishing negative seroprevalence studies not only helps to have more accurate seroprevalence estimates but also allows calculating the specificity of the diagnostic tests used. We performed a population-based Trypanosoma cruzi seroprevalence survey in a community in central Mexico. RESULTS: We surveyed 204 women and children and collected blood by finger prick. We performed rapid tests (Stat-Pak, Chembio, Inc., Medford, New York) and recombinant Chagas ELISA tests v3.0 (Wiener, Rosario, Argentina). All rapid tests and all ELISA tests were negative. CONCLUSION: The rapid test had 100 % of specificity compared to the ELISA.
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spelling pubmed-46259362015-10-30 Negative studies are helpful to compute the specificity of diagnostic tests: measuring Trypanosoma cruzi seroprevalence in Guanajuato, Mexico Padilla-Raygoza, Nicolás Gamboa-León, Rubí Ramirez-Sierra, Maria Jesus Dumonteil, Eric Buekens, Pierre Ruiz-Paloalto, Ma Laura Diaz-Guerrero, Rosalina BMC Res Notes Short Report BACKGROUND: Publishing negative seroprevalence studies not only helps to have more accurate seroprevalence estimates but also allows calculating the specificity of the diagnostic tests used. We performed a population-based Trypanosoma cruzi seroprevalence survey in a community in central Mexico. RESULTS: We surveyed 204 women and children and collected blood by finger prick. We performed rapid tests (Stat-Pak, Chembio, Inc., Medford, New York) and recombinant Chagas ELISA tests v3.0 (Wiener, Rosario, Argentina). All rapid tests and all ELISA tests were negative. CONCLUSION: The rapid test had 100 % of specificity compared to the ELISA. BioMed Central 2015-10-28 /pmc/articles/PMC4625936/ /pubmed/26510987 http://dx.doi.org/10.1186/s13104-015-1612-z Text en © Padilla-Raygoza et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Padilla-Raygoza, Nicolás
Gamboa-León, Rubí
Ramirez-Sierra, Maria Jesus
Dumonteil, Eric
Buekens, Pierre
Ruiz-Paloalto, Ma Laura
Diaz-Guerrero, Rosalina
Negative studies are helpful to compute the specificity of diagnostic tests: measuring Trypanosoma cruzi seroprevalence in Guanajuato, Mexico
title Negative studies are helpful to compute the specificity of diagnostic tests: measuring Trypanosoma cruzi seroprevalence in Guanajuato, Mexico
title_full Negative studies are helpful to compute the specificity of diagnostic tests: measuring Trypanosoma cruzi seroprevalence in Guanajuato, Mexico
title_fullStr Negative studies are helpful to compute the specificity of diagnostic tests: measuring Trypanosoma cruzi seroprevalence in Guanajuato, Mexico
title_full_unstemmed Negative studies are helpful to compute the specificity of diagnostic tests: measuring Trypanosoma cruzi seroprevalence in Guanajuato, Mexico
title_short Negative studies are helpful to compute the specificity of diagnostic tests: measuring Trypanosoma cruzi seroprevalence in Guanajuato, Mexico
title_sort negative studies are helpful to compute the specificity of diagnostic tests: measuring trypanosoma cruzi seroprevalence in guanajuato, mexico
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4625936/
https://www.ncbi.nlm.nih.gov/pubmed/26510987
http://dx.doi.org/10.1186/s13104-015-1612-z
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