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Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development

During development, vagal neural crest cells fated to contribute to the enteric nervous system migrate ventrally away from the neural tube toward and along the primitive gut. The molecular mechanisms that regulate their early migration en route to and entry into the gut remain elusive. Here we show...

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Detalles Bibliográficos
Autores principales: Uribe, Rosa A., Bronner, Marianne E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626059/
https://www.ncbi.nlm.nih.gov/pubmed/26354419
http://dx.doi.org/10.1091/mbc.E15-02-0112
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author Uribe, Rosa A.
Bronner, Marianne E.
author_facet Uribe, Rosa A.
Bronner, Marianne E.
author_sort Uribe, Rosa A.
collection PubMed
description During development, vagal neural crest cells fated to contribute to the enteric nervous system migrate ventrally away from the neural tube toward and along the primitive gut. The molecular mechanisms that regulate their early migration en route to and entry into the gut remain elusive. Here we show that the transcription factor meis3 is expressed along vagal neural crest pathways. Meis3 loss of function results in a reduction in migration efficiency, cell number, and the mitotic activity of neural crest cells in the vicinity of the gut but has no effect on neural crest or gut specification. Later, during enteric nervous system differentiation, Meis3-depleted embryos exhibit colonic aganglionosis, a disorder in which the hindgut is devoid of neurons. Accordingly, the expression of Shh pathway components, previously shown to have a role in the etiology of Hirschsprung’s disease, was misregulated within the gut after loss of Meis3. Taken together, these findings support a model in which Meis3 is required for neural crest proliferation, migration into, and colonization of the gut such that its loss leads to severe defects in enteric nervous system development.
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spelling pubmed-46260592016-01-16 Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development Uribe, Rosa A. Bronner, Marianne E. Mol Biol Cell Articles During development, vagal neural crest cells fated to contribute to the enteric nervous system migrate ventrally away from the neural tube toward and along the primitive gut. The molecular mechanisms that regulate their early migration en route to and entry into the gut remain elusive. Here we show that the transcription factor meis3 is expressed along vagal neural crest pathways. Meis3 loss of function results in a reduction in migration efficiency, cell number, and the mitotic activity of neural crest cells in the vicinity of the gut but has no effect on neural crest or gut specification. Later, during enteric nervous system differentiation, Meis3-depleted embryos exhibit colonic aganglionosis, a disorder in which the hindgut is devoid of neurons. Accordingly, the expression of Shh pathway components, previously shown to have a role in the etiology of Hirschsprung’s disease, was misregulated within the gut after loss of Meis3. Taken together, these findings support a model in which Meis3 is required for neural crest proliferation, migration into, and colonization of the gut such that its loss leads to severe defects in enteric nervous system development. The American Society for Cell Biology 2015-11-01 /pmc/articles/PMC4626059/ /pubmed/26354419 http://dx.doi.org/10.1091/mbc.E15-02-0112 Text en © 2015 Uribe and Bronner. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Uribe, Rosa A.
Bronner, Marianne E.
Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development
title Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development
title_full Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development
title_fullStr Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development
title_full_unstemmed Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development
title_short Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development
title_sort meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626059/
https://www.ncbi.nlm.nih.gov/pubmed/26354419
http://dx.doi.org/10.1091/mbc.E15-02-0112
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