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Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development
During development, vagal neural crest cells fated to contribute to the enteric nervous system migrate ventrally away from the neural tube toward and along the primitive gut. The molecular mechanisms that regulate their early migration en route to and entry into the gut remain elusive. Here we show...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626059/ https://www.ncbi.nlm.nih.gov/pubmed/26354419 http://dx.doi.org/10.1091/mbc.E15-02-0112 |
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author | Uribe, Rosa A. Bronner, Marianne E. |
author_facet | Uribe, Rosa A. Bronner, Marianne E. |
author_sort | Uribe, Rosa A. |
collection | PubMed |
description | During development, vagal neural crest cells fated to contribute to the enteric nervous system migrate ventrally away from the neural tube toward and along the primitive gut. The molecular mechanisms that regulate their early migration en route to and entry into the gut remain elusive. Here we show that the transcription factor meis3 is expressed along vagal neural crest pathways. Meis3 loss of function results in a reduction in migration efficiency, cell number, and the mitotic activity of neural crest cells in the vicinity of the gut but has no effect on neural crest or gut specification. Later, during enteric nervous system differentiation, Meis3-depleted embryos exhibit colonic aganglionosis, a disorder in which the hindgut is devoid of neurons. Accordingly, the expression of Shh pathway components, previously shown to have a role in the etiology of Hirschsprung’s disease, was misregulated within the gut after loss of Meis3. Taken together, these findings support a model in which Meis3 is required for neural crest proliferation, migration into, and colonization of the gut such that its loss leads to severe defects in enteric nervous system development. |
format | Online Article Text |
id | pubmed-4626059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-46260592016-01-16 Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development Uribe, Rosa A. Bronner, Marianne E. Mol Biol Cell Articles During development, vagal neural crest cells fated to contribute to the enteric nervous system migrate ventrally away from the neural tube toward and along the primitive gut. The molecular mechanisms that regulate their early migration en route to and entry into the gut remain elusive. Here we show that the transcription factor meis3 is expressed along vagal neural crest pathways. Meis3 loss of function results in a reduction in migration efficiency, cell number, and the mitotic activity of neural crest cells in the vicinity of the gut but has no effect on neural crest or gut specification. Later, during enteric nervous system differentiation, Meis3-depleted embryos exhibit colonic aganglionosis, a disorder in which the hindgut is devoid of neurons. Accordingly, the expression of Shh pathway components, previously shown to have a role in the etiology of Hirschsprung’s disease, was misregulated within the gut after loss of Meis3. Taken together, these findings support a model in which Meis3 is required for neural crest proliferation, migration into, and colonization of the gut such that its loss leads to severe defects in enteric nervous system development. The American Society for Cell Biology 2015-11-01 /pmc/articles/PMC4626059/ /pubmed/26354419 http://dx.doi.org/10.1091/mbc.E15-02-0112 Text en © 2015 Uribe and Bronner. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Uribe, Rosa A. Bronner, Marianne E. Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development |
title | Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development |
title_full | Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development |
title_fullStr | Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development |
title_full_unstemmed | Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development |
title_short | Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development |
title_sort | meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626059/ https://www.ncbi.nlm.nih.gov/pubmed/26354419 http://dx.doi.org/10.1091/mbc.E15-02-0112 |
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