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Discovery of a vezatin-like protein for dynein-mediated early endosome transport
Early endosomes are transported bidirectionally by cytoplasmic dynein and kinesin-3, but how the movements are regulated in vivo remains unclear. Here our forward genetic study led to the discovery of VezA, a vezatin-like protein in Aspergillus nidulans, as a factor critical for early endosome distr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626066/ https://www.ncbi.nlm.nih.gov/pubmed/26378255 http://dx.doi.org/10.1091/mbc.E15-08-0602 |
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author | Yao, Xuanli Arst, Herbert N. Wang, Xiangfeng Xiang, Xin |
author_facet | Yao, Xuanli Arst, Herbert N. Wang, Xiangfeng Xiang, Xin |
author_sort | Yao, Xuanli |
collection | PubMed |
description | Early endosomes are transported bidirectionally by cytoplasmic dynein and kinesin-3, but how the movements are regulated in vivo remains unclear. Here our forward genetic study led to the discovery of VezA, a vezatin-like protein in Aspergillus nidulans, as a factor critical for early endosome distribution. Loss of vezA causes an abnormal accumulation of early endosomes at the hyphal tip, where microtubule plus ends are located. This abnormal accumulation depends on kinesin-3 and is due to a decrease in the frequency but not the speed of dynein-mediated early endosome movement. VezA-GFP signals are enriched at the hypha tip in an actin-dependent manner but are not obviously associated with early endosomes, thus differing from the early endosome association of the cargo adapter HookA (Hook in A. nidulans). On loss of VezA, HookA associates normally with early endosomes, but the interaction between dynein-dynactin and the early-endosome-bound HookA is significantly decreased. However, VezA is not required for linking dynein-dynactin to the cytosolic ∆C-HookA, lacking the cargo-binding C-terminus. These results identify VezA as a novel regulator required for the interaction between dynein and the Hook-bound early endosomes in vivo. |
format | Online Article Text |
id | pubmed-4626066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-46260662016-01-16 Discovery of a vezatin-like protein for dynein-mediated early endosome transport Yao, Xuanli Arst, Herbert N. Wang, Xiangfeng Xiang, Xin Mol Biol Cell Articles Early endosomes are transported bidirectionally by cytoplasmic dynein and kinesin-3, but how the movements are regulated in vivo remains unclear. Here our forward genetic study led to the discovery of VezA, a vezatin-like protein in Aspergillus nidulans, as a factor critical for early endosome distribution. Loss of vezA causes an abnormal accumulation of early endosomes at the hyphal tip, where microtubule plus ends are located. This abnormal accumulation depends on kinesin-3 and is due to a decrease in the frequency but not the speed of dynein-mediated early endosome movement. VezA-GFP signals are enriched at the hypha tip in an actin-dependent manner but are not obviously associated with early endosomes, thus differing from the early endosome association of the cargo adapter HookA (Hook in A. nidulans). On loss of VezA, HookA associates normally with early endosomes, but the interaction between dynein-dynactin and the early-endosome-bound HookA is significantly decreased. However, VezA is not required for linking dynein-dynactin to the cytosolic ∆C-HookA, lacking the cargo-binding C-terminus. These results identify VezA as a novel regulator required for the interaction between dynein and the Hook-bound early endosomes in vivo. The American Society for Cell Biology 2015-11-01 /pmc/articles/PMC4626066/ /pubmed/26378255 http://dx.doi.org/10.1091/mbc.E15-08-0602 Text en © 2015 Yao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Yao, Xuanli Arst, Herbert N. Wang, Xiangfeng Xiang, Xin Discovery of a vezatin-like protein for dynein-mediated early endosome transport |
title | Discovery of a vezatin-like protein for dynein-mediated early endosome transport |
title_full | Discovery of a vezatin-like protein for dynein-mediated early endosome transport |
title_fullStr | Discovery of a vezatin-like protein for dynein-mediated early endosome transport |
title_full_unstemmed | Discovery of a vezatin-like protein for dynein-mediated early endosome transport |
title_short | Discovery of a vezatin-like protein for dynein-mediated early endosome transport |
title_sort | discovery of a vezatin-like protein for dynein-mediated early endosome transport |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626066/ https://www.ncbi.nlm.nih.gov/pubmed/26378255 http://dx.doi.org/10.1091/mbc.E15-08-0602 |
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