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Discovery of a vezatin-like protein for dynein-mediated early endosome transport

Early endosomes are transported bidirectionally by cytoplasmic dynein and kinesin-3, but how the movements are regulated in vivo remains unclear. Here our forward genetic study led to the discovery of VezA, a vezatin-like protein in Aspergillus nidulans, as a factor critical for early endosome distr...

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Autores principales: Yao, Xuanli, Arst, Herbert N., Wang, Xiangfeng, Xiang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626066/
https://www.ncbi.nlm.nih.gov/pubmed/26378255
http://dx.doi.org/10.1091/mbc.E15-08-0602
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author Yao, Xuanli
Arst, Herbert N.
Wang, Xiangfeng
Xiang, Xin
author_facet Yao, Xuanli
Arst, Herbert N.
Wang, Xiangfeng
Xiang, Xin
author_sort Yao, Xuanli
collection PubMed
description Early endosomes are transported bidirectionally by cytoplasmic dynein and kinesin-3, but how the movements are regulated in vivo remains unclear. Here our forward genetic study led to the discovery of VezA, a vezatin-like protein in Aspergillus nidulans, as a factor critical for early endosome distribution. Loss of vezA causes an abnormal accumulation of early endosomes at the hyphal tip, where microtubule plus ends are located. This abnormal accumulation depends on kinesin-3 and is due to a decrease in the frequency but not the speed of dynein-mediated early endosome movement. VezA-GFP signals are enriched at the hypha tip in an actin-dependent manner but are not obviously associated with early endosomes, thus differing from the early endosome association of the cargo adapter HookA (Hook in A. nidulans). On loss of VezA, HookA associates normally with early endosomes, but the interaction between dynein-dynactin and the early-endosome-bound HookA is significantly decreased. However, VezA is not required for linking dynein-dynactin to the cytosolic ∆C-HookA, lacking the cargo-binding C-terminus. These results identify VezA as a novel regulator required for the interaction between dynein and the Hook-bound early endosomes in vivo.
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spelling pubmed-46260662016-01-16 Discovery of a vezatin-like protein for dynein-mediated early endosome transport Yao, Xuanli Arst, Herbert N. Wang, Xiangfeng Xiang, Xin Mol Biol Cell Articles Early endosomes are transported bidirectionally by cytoplasmic dynein and kinesin-3, but how the movements are regulated in vivo remains unclear. Here our forward genetic study led to the discovery of VezA, a vezatin-like protein in Aspergillus nidulans, as a factor critical for early endosome distribution. Loss of vezA causes an abnormal accumulation of early endosomes at the hyphal tip, where microtubule plus ends are located. This abnormal accumulation depends on kinesin-3 and is due to a decrease in the frequency but not the speed of dynein-mediated early endosome movement. VezA-GFP signals are enriched at the hypha tip in an actin-dependent manner but are not obviously associated with early endosomes, thus differing from the early endosome association of the cargo adapter HookA (Hook in A. nidulans). On loss of VezA, HookA associates normally with early endosomes, but the interaction between dynein-dynactin and the early-endosome-bound HookA is significantly decreased. However, VezA is not required for linking dynein-dynactin to the cytosolic ∆C-HookA, lacking the cargo-binding C-terminus. These results identify VezA as a novel regulator required for the interaction between dynein and the Hook-bound early endosomes in vivo. The American Society for Cell Biology 2015-11-01 /pmc/articles/PMC4626066/ /pubmed/26378255 http://dx.doi.org/10.1091/mbc.E15-08-0602 Text en © 2015 Yao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Yao, Xuanli
Arst, Herbert N.
Wang, Xiangfeng
Xiang, Xin
Discovery of a vezatin-like protein for dynein-mediated early endosome transport
title Discovery of a vezatin-like protein for dynein-mediated early endosome transport
title_full Discovery of a vezatin-like protein for dynein-mediated early endosome transport
title_fullStr Discovery of a vezatin-like protein for dynein-mediated early endosome transport
title_full_unstemmed Discovery of a vezatin-like protein for dynein-mediated early endosome transport
title_short Discovery of a vezatin-like protein for dynein-mediated early endosome transport
title_sort discovery of a vezatin-like protein for dynein-mediated early endosome transport
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626066/
https://www.ncbi.nlm.nih.gov/pubmed/26378255
http://dx.doi.org/10.1091/mbc.E15-08-0602
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