Bisulfite-free and Base-resolution Analysis of 5-formylcytosine at Whole-genome Scale

Active DNA demethylation in mammals involves TET-mediated oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxycytosine (5caC). However, genome-wide detection of 5fC at single-base resolution remains challenging. Here we present a bisulfite-free...

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Detalles Bibliográficos
Autores principales: Xia, Bo, Han, Dali, Lu, Xingyu, Sun, Zhaozhu, Zhou, Ankun, Yin, Qiangzong, Zeng, Hu, Liu, Menghao, Jiang, Xiang, Xie, Wei, He, Chuan, Yi, Chengqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626315/
https://www.ncbi.nlm.nih.gov/pubmed/26344045
http://dx.doi.org/10.1038/nmeth.3569
Descripción
Sumario:Active DNA demethylation in mammals involves TET-mediated oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxycytosine (5caC). However, genome-wide detection of 5fC at single-base resolution remains challenging. Here we present a bisulfite-free method for whole-genome analysis of 5fC, based on selective chemical labeling of 5fC and subsequent C-to-T transition during PCR. Base-resolution 5fC maps reveal limited overlap with 5hmC, with 5fC-marked regions more active than 5hmC-marked ones.

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