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WASP: allele-specific software for robust molecular quantitative trait locus discovery

Allele-specific sequencing reads provide a powerful signal for identifying molecular quantitative trait loci (QTLs), however they are challenging to analyze and prone to technical artefacts. Here we describe WASP, a suite of tools for unbiased allele-specific read mapping and discovery of molecular...

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Detalles Bibliográficos
Autores principales: van de Geijn, Bryce, McVicker, Graham, Gilad, Yoav, Pritchard, Jonathan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626402/
https://www.ncbi.nlm.nih.gov/pubmed/26366987
http://dx.doi.org/10.1038/nmeth.3582
Descripción
Sumario:Allele-specific sequencing reads provide a powerful signal for identifying molecular quantitative trait loci (QTLs), however they are challenging to analyze and prone to technical artefacts. Here we describe WASP, a suite of tools for unbiased allele-specific read mapping and discovery of molecular QTLs. Using simulated reads, RNA-seq reads and ChIP-seq reads, we demonstrate that WASP has a low error rate and is far more powerful than existing QTL mapping approaches.