Cargando…

Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation

Marine diatoms have recently gained much attention as they are expected to be a promising resource for sustainable production of bioactive compounds such as carotenoids and biofuels as a future clean energy solution. To develop photosynthetic cell factories, it is important to improve diatoms for va...

Descripción completa

Detalles Bibliográficos
Autores principales: Yi, Zhiqian, Xu, Maonian, Magnusdottir, Manuela, Zhang, Yuetuan, Brynjolfsson, Sigurdur, Fu, Weiqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626683/
https://www.ncbi.nlm.nih.gov/pubmed/26426027
http://dx.doi.org/10.3390/md13106138
_version_ 1782398142480646144
author Yi, Zhiqian
Xu, Maonian
Magnusdottir, Manuela
Zhang, Yuetuan
Brynjolfsson, Sigurdur
Fu, Weiqi
author_facet Yi, Zhiqian
Xu, Maonian
Magnusdottir, Manuela
Zhang, Yuetuan
Brynjolfsson, Sigurdur
Fu, Weiqi
author_sort Yi, Zhiqian
collection PubMed
description Marine diatoms have recently gained much attention as they are expected to be a promising resource for sustainable production of bioactive compounds such as carotenoids and biofuels as a future clean energy solution. To develop photosynthetic cell factories, it is important to improve diatoms for value-added products. In this study, we utilized UVC radiation to induce mutations in the marine diatom Phaeodactylum tricornutum and screened strains with enhanced accumulation of neutral lipids and carotenoids. Adaptive laboratory evolution (ALE) was also used in parallel to develop altered phenotypic and biological functions in P. tricornutum and it was reported for the first time that ALE was successfully applied on diatoms for the enhancement of growth performance and productivity of value-added carotenoids to date. Liquid chromatography-mass spectrometry (LC-MS) was utilized to study the composition of major pigments in the wild type P. tricornutum, UV mutants and ALE strains. UVC radiated strains exhibited higher accumulation of fucoxanthin as well as neutral lipids compared to their wild type counterpart. In addition to UV mutagenesis, P. tricornutum strains developed by ALE also yielded enhanced biomass production and fucoxanthin accumulation under combined red and blue light. In short, both UV mutagenesis and ALE appeared as an effective approach to developing desired phenotypes in the marine diatoms via electromagnetic radiation-induced oxidative stress.
format Online
Article
Text
id pubmed-4626683
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-46266832015-11-12 Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation Yi, Zhiqian Xu, Maonian Magnusdottir, Manuela Zhang, Yuetuan Brynjolfsson, Sigurdur Fu, Weiqi Mar Drugs Article Marine diatoms have recently gained much attention as they are expected to be a promising resource for sustainable production of bioactive compounds such as carotenoids and biofuels as a future clean energy solution. To develop photosynthetic cell factories, it is important to improve diatoms for value-added products. In this study, we utilized UVC radiation to induce mutations in the marine diatom Phaeodactylum tricornutum and screened strains with enhanced accumulation of neutral lipids and carotenoids. Adaptive laboratory evolution (ALE) was also used in parallel to develop altered phenotypic and biological functions in P. tricornutum and it was reported for the first time that ALE was successfully applied on diatoms for the enhancement of growth performance and productivity of value-added carotenoids to date. Liquid chromatography-mass spectrometry (LC-MS) was utilized to study the composition of major pigments in the wild type P. tricornutum, UV mutants and ALE strains. UVC radiated strains exhibited higher accumulation of fucoxanthin as well as neutral lipids compared to their wild type counterpart. In addition to UV mutagenesis, P. tricornutum strains developed by ALE also yielded enhanced biomass production and fucoxanthin accumulation under combined red and blue light. In short, both UV mutagenesis and ALE appeared as an effective approach to developing desired phenotypes in the marine diatoms via electromagnetic radiation-induced oxidative stress. MDPI 2015-09-29 /pmc/articles/PMC4626683/ /pubmed/26426027 http://dx.doi.org/10.3390/md13106138 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yi, Zhiqian
Xu, Maonian
Magnusdottir, Manuela
Zhang, Yuetuan
Brynjolfsson, Sigurdur
Fu, Weiqi
Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation
title Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation
title_full Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation
title_fullStr Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation
title_full_unstemmed Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation
title_short Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation
title_sort photo-oxidative stress-driven mutagenesis and adaptive evolution on the marine diatom phaeodactylum tricornutum for enhanced carotenoid accumulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626683/
https://www.ncbi.nlm.nih.gov/pubmed/26426027
http://dx.doi.org/10.3390/md13106138
work_keys_str_mv AT yizhiqian photooxidativestressdrivenmutagenesisandadaptiveevolutiononthemarinediatomphaeodactylumtricornutumforenhancedcarotenoidaccumulation
AT xumaonian photooxidativestressdrivenmutagenesisandadaptiveevolutiononthemarinediatomphaeodactylumtricornutumforenhancedcarotenoidaccumulation
AT magnusdottirmanuela photooxidativestressdrivenmutagenesisandadaptiveevolutiononthemarinediatomphaeodactylumtricornutumforenhancedcarotenoidaccumulation
AT zhangyuetuan photooxidativestressdrivenmutagenesisandadaptiveevolutiononthemarinediatomphaeodactylumtricornutumforenhancedcarotenoidaccumulation
AT brynjolfssonsigurdur photooxidativestressdrivenmutagenesisandadaptiveevolutiononthemarinediatomphaeodactylumtricornutumforenhancedcarotenoidaccumulation
AT fuweiqi photooxidativestressdrivenmutagenesisandadaptiveevolutiononthemarinediatomphaeodactylumtricornutumforenhancedcarotenoidaccumulation