Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan

For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concent...

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Autores principales: Kameneva, Polina A., Efimova, Kseniya V., Rybin, Viacheslav G., Orlova, Tatiana Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626713/
https://www.ncbi.nlm.nih.gov/pubmed/26426049
http://dx.doi.org/10.3390/toxins7103947
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author Kameneva, Polina A.
Efimova, Kseniya V.
Rybin, Viacheslav G.
Orlova, Tatiana Y.
author_facet Kameneva, Polina A.
Efimova, Kseniya V.
Rybin, Viacheslav G.
Orlova, Tatiana Y.
author_sort Kameneva, Polina A.
collection PubMed
description For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry.
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spelling pubmed-46267132015-11-12 Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan Kameneva, Polina A. Efimova, Kseniya V. Rybin, Viacheslav G. Orlova, Tatiana Y. Toxins (Basel) Communication For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry. MDPI 2015-09-28 /pmc/articles/PMC4626713/ /pubmed/26426049 http://dx.doi.org/10.3390/toxins7103947 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kameneva, Polina A.
Efimova, Kseniya V.
Rybin, Viacheslav G.
Orlova, Tatiana Y.
Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_full Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_fullStr Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_full_unstemmed Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_short Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
title_sort detection of dinophysistoxin-1 in clonal culture of marine dinoflagellate prorocentrum foraminosum (faust m.a., 1993) from the sea of japan
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626713/
https://www.ncbi.nlm.nih.gov/pubmed/26426049
http://dx.doi.org/10.3390/toxins7103947
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