Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak

Detection of Shiga toxins (Stx) is important for accurate diagnosis of Enterohemorrhagic Escherichia coli infection. In this study, we quantitatively analyzed Stx protein in nine patients’ stool during an outbreak that occurred in Japan. Highly sensitive immunoassay (bead enzyme-linked immunosorbent...

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Autores principales: Yamasaki, Eiki, Watahiki, Masanori, Isobe, Junko, Sata, Tetsutaro, Nair, G. Balakrish, Kurazono, Hisao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626740/
https://www.ncbi.nlm.nih.gov/pubmed/26516915
http://dx.doi.org/10.3390/toxins7104381
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author Yamasaki, Eiki
Watahiki, Masanori
Isobe, Junko
Sata, Tetsutaro
Nair, G. Balakrish
Kurazono, Hisao
author_facet Yamasaki, Eiki
Watahiki, Masanori
Isobe, Junko
Sata, Tetsutaro
Nair, G. Balakrish
Kurazono, Hisao
author_sort Yamasaki, Eiki
collection PubMed
description Detection of Shiga toxins (Stx) is important for accurate diagnosis of Enterohemorrhagic Escherichia coli infection. In this study, we quantitatively analyzed Stx protein in nine patients’ stool during an outbreak that occurred in Japan. Highly sensitive immunoassay (bead enzyme-linked immunosorbent assay (bead-ELISA)) revealed that the concentrations of toxins in stool of patients ranged from 0.71 to 10.44 ng/mL for Stx1 and 2.75 to 51.61 ng/mL for Stx2. To our knowledge, this is the first report that reveals the range of Stx protein concentrations in human stools.
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spelling pubmed-46267402015-11-12 Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak Yamasaki, Eiki Watahiki, Masanori Isobe, Junko Sata, Tetsutaro Nair, G. Balakrish Kurazono, Hisao Toxins (Basel) Brief Report Detection of Shiga toxins (Stx) is important for accurate diagnosis of Enterohemorrhagic Escherichia coli infection. In this study, we quantitatively analyzed Stx protein in nine patients’ stool during an outbreak that occurred in Japan. Highly sensitive immunoassay (bead enzyme-linked immunosorbent assay (bead-ELISA)) revealed that the concentrations of toxins in stool of patients ranged from 0.71 to 10.44 ng/mL for Stx1 and 2.75 to 51.61 ng/mL for Stx2. To our knowledge, this is the first report that reveals the range of Stx protein concentrations in human stools. MDPI 2015-10-27 /pmc/articles/PMC4626740/ /pubmed/26516915 http://dx.doi.org/10.3390/toxins7104381 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Yamasaki, Eiki
Watahiki, Masanori
Isobe, Junko
Sata, Tetsutaro
Nair, G. Balakrish
Kurazono, Hisao
Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak
title Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak
title_full Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak
title_fullStr Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak
title_full_unstemmed Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak
title_short Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak
title_sort quantitative detection of shiga toxins directly from stool specimens of patients associated with an outbreak of enterohemorrhagic escherichia coli in japan—quantitative shiga toxin detection from stool during ehec outbreak
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626740/
https://www.ncbi.nlm.nih.gov/pubmed/26516915
http://dx.doi.org/10.3390/toxins7104381
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