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Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo

The Piwi-interacting RNA (piRNA) pathway plays an essential role in the repression of transposons in the germline. Other functions of piRNAs such as post-transcriptional regulation of mRNAs are now emerging. Here, we perform iCLIP with the PIWI protein Aubergine (Aub) and identify hundreds of matern...

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Autores principales: Barckmann, Bridlin, Pierson, Stéphanie, Dufourt, Jérémy, Papin, Catherine, Armenise, Claudia, Port, Fillip, Grentzinger, Thomas, Chambeyron, Séverine, Baronian, Grégory, Desvignes, Jean-Pierre, Curk, Tomaz, Simonelig, Martine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626872/
https://www.ncbi.nlm.nih.gov/pubmed/26257181
http://dx.doi.org/10.1016/j.celrep.2015.07.030
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author Barckmann, Bridlin
Pierson, Stéphanie
Dufourt, Jérémy
Papin, Catherine
Armenise, Claudia
Port, Fillip
Grentzinger, Thomas
Chambeyron, Séverine
Baronian, Grégory
Desvignes, Jean-Pierre
Curk, Tomaz
Simonelig, Martine
author_facet Barckmann, Bridlin
Pierson, Stéphanie
Dufourt, Jérémy
Papin, Catherine
Armenise, Claudia
Port, Fillip
Grentzinger, Thomas
Chambeyron, Séverine
Baronian, Grégory
Desvignes, Jean-Pierre
Curk, Tomaz
Simonelig, Martine
author_sort Barckmann, Bridlin
collection PubMed
description The Piwi-interacting RNA (piRNA) pathway plays an essential role in the repression of transposons in the germline. Other functions of piRNAs such as post-transcriptional regulation of mRNAs are now emerging. Here, we perform iCLIP with the PIWI protein Aubergine (Aub) and identify hundreds of maternal mRNAs interacting with Aub in the early Drosophila embryo. Gene expression profiling reveals that a proportion of these mRNAs undergo Aub-dependent destabilization during the maternal-to-zygotic transition. Strikingly, Aub-dependent unstable mRNAs encode germ cell determinants. iCLIP with an Aub mutant that is unable to bind piRNAs confirms piRNA-dependent binding of Aub to mRNAs. Base pairing between piRNAs and mRNAs can induce mRNA cleavage and decay that are essential for embryonic development. These results suggest general regulation of maternal mRNAs by Aub and piRNAs, which plays a key developmental role in the embryo through decay and localization of mRNAs encoding germ cell determinants.
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spelling pubmed-46268722015-11-30 Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo Barckmann, Bridlin Pierson, Stéphanie Dufourt, Jérémy Papin, Catherine Armenise, Claudia Port, Fillip Grentzinger, Thomas Chambeyron, Séverine Baronian, Grégory Desvignes, Jean-Pierre Curk, Tomaz Simonelig, Martine Cell Rep Resource The Piwi-interacting RNA (piRNA) pathway plays an essential role in the repression of transposons in the germline. Other functions of piRNAs such as post-transcriptional regulation of mRNAs are now emerging. Here, we perform iCLIP with the PIWI protein Aubergine (Aub) and identify hundreds of maternal mRNAs interacting with Aub in the early Drosophila embryo. Gene expression profiling reveals that a proportion of these mRNAs undergo Aub-dependent destabilization during the maternal-to-zygotic transition. Strikingly, Aub-dependent unstable mRNAs encode germ cell determinants. iCLIP with an Aub mutant that is unable to bind piRNAs confirms piRNA-dependent binding of Aub to mRNAs. Base pairing between piRNAs and mRNAs can induce mRNA cleavage and decay that are essential for embryonic development. These results suggest general regulation of maternal mRNAs by Aub and piRNAs, which plays a key developmental role in the embryo through decay and localization of mRNAs encoding germ cell determinants. Cell Press 2015-08-06 /pmc/articles/PMC4626872/ /pubmed/26257181 http://dx.doi.org/10.1016/j.celrep.2015.07.030 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Resource
Barckmann, Bridlin
Pierson, Stéphanie
Dufourt, Jérémy
Papin, Catherine
Armenise, Claudia
Port, Fillip
Grentzinger, Thomas
Chambeyron, Séverine
Baronian, Grégory
Desvignes, Jean-Pierre
Curk, Tomaz
Simonelig, Martine
Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo
title Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo
title_full Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo
title_fullStr Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo
title_full_unstemmed Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo
title_short Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo
title_sort aubergine iclip reveals pirna-dependent decay of mrnas involved in germ cell development in the early embryo
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626872/
https://www.ncbi.nlm.nih.gov/pubmed/26257181
http://dx.doi.org/10.1016/j.celrep.2015.07.030
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