Cargando…

Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF

Bisphenol AF (BPAF) is extensively used as a raw material in industry, resulting in its widespread distribution in the aqueous environment. However, the effect of BPAF on the hypothalamic-pituitary-thyroidal (HPT) axis remains unknown. For elucidating the disruptive effects of BPAF on thyroid functi...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Tianle, Yang, Yang, Chen, Yawen, Tang, Wenhao, Wang, Fuqiang, Diao, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627017/
https://www.ncbi.nlm.nih.gov/pubmed/26501309
http://dx.doi.org/10.3390/ijerph121013069
_version_ 1782398204510208000
author Tang, Tianle
Yang, Yang
Chen, Yawen
Tang, Wenhao
Wang, Fuqiang
Diao, Xiaoping
author_facet Tang, Tianle
Yang, Yang
Chen, Yawen
Tang, Wenhao
Wang, Fuqiang
Diao, Xiaoping
author_sort Tang, Tianle
collection PubMed
description Bisphenol AF (BPAF) is extensively used as a raw material in industry, resulting in its widespread distribution in the aqueous environment. However, the effect of BPAF on the hypothalamic-pituitary-thyroidal (HPT) axis remains unknown. For elucidating the disruptive effects of BPAF on thyroid function and expression of the representative genes along the HPT axis in zebrafish (Danio rerio) embryos, whole-body total 3,3′,5-triiodothyronine (TT3), total 3,5,3′,5′-tetraiodothyronine (TT4), free 3,3′,5-triiodothyronine (FT3) and free 3,5,3′,5′-tetraiodothyronine (FT4) levels were examined following 168 h post-fertilization exposure to different BPAF concentrations (0, 5, 50 and 500 μg/L). The results showed that whole-body TT3, TT4, FT3 and FT4 contents decreased significantly with the BPAF treatment, indicating an endocrine disruption of thyroid. The expression of thyroid-stimulating hormone-β and thyroglobulin genes increased after exposing to 50 μg/L BPAF in seven-day-old larvae. The expressions of thyronine deiodinases type 1, type 2 and transthyretin mRNAs were also significantly up-regulated, which were possibly associated with a deterioration of thyroid function. However, slc5a5 gene transcription was significantly down-regulated at 50 μg/L and 500 μg/L BPAF exposure. Furthermore, trα and trβ genes were down-regulated transcriptionally after BPAF exposure. It demonstrates that BPAF exposure triggered thyroid endocrine toxicity by altering the whole-body contents of thyroid hormones and changing the transcription of the genes involved in the HPT axis in zebrafish larvae.
format Online
Article
Text
id pubmed-4627017
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-46270172015-11-12 Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF Tang, Tianle Yang, Yang Chen, Yawen Tang, Wenhao Wang, Fuqiang Diao, Xiaoping Int J Environ Res Public Health Article Bisphenol AF (BPAF) is extensively used as a raw material in industry, resulting in its widespread distribution in the aqueous environment. However, the effect of BPAF on the hypothalamic-pituitary-thyroidal (HPT) axis remains unknown. For elucidating the disruptive effects of BPAF on thyroid function and expression of the representative genes along the HPT axis in zebrafish (Danio rerio) embryos, whole-body total 3,3′,5-triiodothyronine (TT3), total 3,5,3′,5′-tetraiodothyronine (TT4), free 3,3′,5-triiodothyronine (FT3) and free 3,5,3′,5′-tetraiodothyronine (FT4) levels were examined following 168 h post-fertilization exposure to different BPAF concentrations (0, 5, 50 and 500 μg/L). The results showed that whole-body TT3, TT4, FT3 and FT4 contents decreased significantly with the BPAF treatment, indicating an endocrine disruption of thyroid. The expression of thyroid-stimulating hormone-β and thyroglobulin genes increased after exposing to 50 μg/L BPAF in seven-day-old larvae. The expressions of thyronine deiodinases type 1, type 2 and transthyretin mRNAs were also significantly up-regulated, which were possibly associated with a deterioration of thyroid function. However, slc5a5 gene transcription was significantly down-regulated at 50 μg/L and 500 μg/L BPAF exposure. Furthermore, trα and trβ genes were down-regulated transcriptionally after BPAF exposure. It demonstrates that BPAF exposure triggered thyroid endocrine toxicity by altering the whole-body contents of thyroid hormones and changing the transcription of the genes involved in the HPT axis in zebrafish larvae. MDPI 2015-10-16 2015-10 /pmc/articles/PMC4627017/ /pubmed/26501309 http://dx.doi.org/10.3390/ijerph121013069 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Tianle
Yang, Yang
Chen, Yawen
Tang, Wenhao
Wang, Fuqiang
Diao, Xiaoping
Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF
title Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF
title_full Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF
title_fullStr Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF
title_full_unstemmed Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF
title_short Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF
title_sort thyroid disruption in zebrafish larvae by short-term exposure to bisphenol af
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627017/
https://www.ncbi.nlm.nih.gov/pubmed/26501309
http://dx.doi.org/10.3390/ijerph121013069
work_keys_str_mv AT tangtianle thyroiddisruptioninzebrafishlarvaebyshorttermexposuretobisphenolaf
AT yangyang thyroiddisruptioninzebrafishlarvaebyshorttermexposuretobisphenolaf
AT chenyawen thyroiddisruptioninzebrafishlarvaebyshorttermexposuretobisphenolaf
AT tangwenhao thyroiddisruptioninzebrafishlarvaebyshorttermexposuretobisphenolaf
AT wangfuqiang thyroiddisruptioninzebrafishlarvaebyshorttermexposuretobisphenolaf
AT diaoxiaoping thyroiddisruptioninzebrafishlarvaebyshorttermexposuretobisphenolaf