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Efficient conditional knockout targeting vector construction using co-selection BAC recombineering (CoSBR)
A simple and efficient strategy for Bacterial Artificial Chromosome (BAC) recombineering based on co-selection is described. We show that it is possible to efficiently modify two positions of a BAC simultaneously by co-transformation of a single-stranded DNA oligo and a double-stranded selection cas...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627060/ https://www.ncbi.nlm.nih.gov/pubmed/26089387 http://dx.doi.org/10.1093/nar/gkv600 |