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Sp1-driven up-regulation of miR-19a decreases RHOB and promotes pancreatic cancer
Cancer treatment alters microRNA (miRNA) expression, revealing potential therapeutic targets (oncotarget). Here we treated pancreatic cancer (ASPC-1) cells with either recombinant human endostatin (rh-endostatin) or gemcitabine. Then high-throughput sequencing assay was performed to screen for alter...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627316/ https://www.ncbi.nlm.nih.gov/pubmed/26041879 |
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author | Tan, Yonggang Yin, Hongzhuan Zhang, Heying Fang, Jun Zheng, Wei Li, Dan Li, Yue Cao, Wei Sun, Cheng Liang, Yusi Zeng, Juan Zou, Huawei Fu, Weineng Yang, Xianghong |
author_facet | Tan, Yonggang Yin, Hongzhuan Zhang, Heying Fang, Jun Zheng, Wei Li, Dan Li, Yue Cao, Wei Sun, Cheng Liang, Yusi Zeng, Juan Zou, Huawei Fu, Weineng Yang, Xianghong |
author_sort | Tan, Yonggang |
collection | PubMed |
description | Cancer treatment alters microRNA (miRNA) expression, revealing potential therapeutic targets (oncotarget). Here we treated pancreatic cancer (ASPC-1) cells with either recombinant human endostatin (rh-endostatin) or gemcitabine. Then high-throughput sequencing assay was performed to screen for altered miRNAs. Both treatments decreased levels of MiR-19a. We found that miR-19a stimulated cell proliferation, migration, invasion in vitro and tumor growth in vivo. High levels of miR-19a correlated with poor prognosis in patients. Ras homolog family member B (RHOB) was identified as a direct target of miR-19a. Furthermore, RHOB was down-regulated in human pancreatic cancer samples. Restoration of RHOB induced apoptosis, inhibited proliferation and migration of ASPC-1 cells. SP-1 was identified as an upstream transcription factor of miR-19a gene, promoting miR-19a transcription. Rh-endostatin decreased miR-19a expression by down-regulating SP-1. These findings suggest that miR-19a is a potential therapeutic target in pancreatic cancer. |
format | Online Article Text |
id | pubmed-4627316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-46273162015-12-02 Sp1-driven up-regulation of miR-19a decreases RHOB and promotes pancreatic cancer Tan, Yonggang Yin, Hongzhuan Zhang, Heying Fang, Jun Zheng, Wei Li, Dan Li, Yue Cao, Wei Sun, Cheng Liang, Yusi Zeng, Juan Zou, Huawei Fu, Weineng Yang, Xianghong Oncotarget Research Paper Cancer treatment alters microRNA (miRNA) expression, revealing potential therapeutic targets (oncotarget). Here we treated pancreatic cancer (ASPC-1) cells with either recombinant human endostatin (rh-endostatin) or gemcitabine. Then high-throughput sequencing assay was performed to screen for altered miRNAs. Both treatments decreased levels of MiR-19a. We found that miR-19a stimulated cell proliferation, migration, invasion in vitro and tumor growth in vivo. High levels of miR-19a correlated with poor prognosis in patients. Ras homolog family member B (RHOB) was identified as a direct target of miR-19a. Furthermore, RHOB was down-regulated in human pancreatic cancer samples. Restoration of RHOB induced apoptosis, inhibited proliferation and migration of ASPC-1 cells. SP-1 was identified as an upstream transcription factor of miR-19a gene, promoting miR-19a transcription. Rh-endostatin decreased miR-19a expression by down-regulating SP-1. These findings suggest that miR-19a is a potential therapeutic target in pancreatic cancer. Impact Journals LLC 2015-05-25 /pmc/articles/PMC4627316/ /pubmed/26041879 Text en Copyright: © 2015 Tan et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Tan, Yonggang Yin, Hongzhuan Zhang, Heying Fang, Jun Zheng, Wei Li, Dan Li, Yue Cao, Wei Sun, Cheng Liang, Yusi Zeng, Juan Zou, Huawei Fu, Weineng Yang, Xianghong Sp1-driven up-regulation of miR-19a decreases RHOB and promotes pancreatic cancer |
title | Sp1-driven up-regulation of miR-19a decreases RHOB and promotes pancreatic cancer |
title_full | Sp1-driven up-regulation of miR-19a decreases RHOB and promotes pancreatic cancer |
title_fullStr | Sp1-driven up-regulation of miR-19a decreases RHOB and promotes pancreatic cancer |
title_full_unstemmed | Sp1-driven up-regulation of miR-19a decreases RHOB and promotes pancreatic cancer |
title_short | Sp1-driven up-regulation of miR-19a decreases RHOB and promotes pancreatic cancer |
title_sort | sp1-driven up-regulation of mir-19a decreases rhob and promotes pancreatic cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627316/ https://www.ncbi.nlm.nih.gov/pubmed/26041879 |
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