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Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis
DNA methylation reprogramming occurs during mammalian gametogenesis and embryogenesis. Sex-specific DNA methylation patterns at specific CpG islands controlling imprinted genes are acquired during this window of development. Characterization of the DNA methylation dynamics of imprinted genes acquire...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627647/ https://www.ncbi.nlm.nih.gov/pubmed/26517264 http://dx.doi.org/10.1371/journal.pone.0142072 |
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author | Mendonça, Anelise dos Santos Guimarães, Ana Luíza Silva da Silva, Naiara Milagres Augusto Caetano, Alexandre Rodrigues Dode, Margot Alves Nunes Franco, Maurício Machaim |
author_facet | Mendonça, Anelise dos Santos Guimarães, Ana Luíza Silva da Silva, Naiara Milagres Augusto Caetano, Alexandre Rodrigues Dode, Margot Alves Nunes Franco, Maurício Machaim |
author_sort | Mendonça, Anelise dos Santos |
collection | PubMed |
description | DNA methylation reprogramming occurs during mammalian gametogenesis and embryogenesis. Sex-specific DNA methylation patterns at specific CpG islands controlling imprinted genes are acquired during this window of development. Characterization of the DNA methylation dynamics of imprinted genes acquired by oocytes during folliculogenesis is essential for understanding the physiological and genetic aspects of female gametogenesis and to determine the parameters for oocyte competence. This knowledge can be used to improve in vitro embryo production (IVP), specifically because oocyte competence is one of the most important aspects determining the success of IVP. Imprinted genes, such as IGF2, play important roles in embryo development, placentation and fetal growth. The aim of this study was to characterize the DNA methylation profile of the CpG island located in IGF2 exon 10 in oocytes during bovine folliculogenesis. The methylation percentages in oocytes from primordial follicles, final secondary follicles, small antral follicles, large antral follicles, MII oocytes and spermatozoa were 73.74 ± 2.88%, 58.70 ± 7.46%, 56.00 ± 5.58%, 65.77 ± 5.10%, 56.35 ± 7.45% and 96.04 ± 0.78%, respectively. Oocytes from primordial follicles showed fewer hypomethylated alleles (15.5%) than MII oocytes (34.6%) (p = 0.039); spermatozoa showed only hypermethylated alleles. Moreover, MII oocytes were less methylated than spermatozoa (p<0.001). Our results showed that the methylation pattern of this region behaves differently between mature oocytes and spermatozoa. However, while this region has a classical imprinted pattern in spermatozoa that is fully methylated, it was variable in mature oocytes, showing hypermethylated and hypomethylated alleles. Furthermore, our results suggest that this CpG island may have received precocious reprogramming, considering that the hypermethylated pattern was already found in growing oocytes from primordial follicles. These results may contribute to our understanding of the reprogramming of imprinted genes during bovine oogenesis. |
format | Online Article Text |
id | pubmed-4627647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46276472015-11-06 Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis Mendonça, Anelise dos Santos Guimarães, Ana Luíza Silva da Silva, Naiara Milagres Augusto Caetano, Alexandre Rodrigues Dode, Margot Alves Nunes Franco, Maurício Machaim PLoS One Research Article DNA methylation reprogramming occurs during mammalian gametogenesis and embryogenesis. Sex-specific DNA methylation patterns at specific CpG islands controlling imprinted genes are acquired during this window of development. Characterization of the DNA methylation dynamics of imprinted genes acquired by oocytes during folliculogenesis is essential for understanding the physiological and genetic aspects of female gametogenesis and to determine the parameters for oocyte competence. This knowledge can be used to improve in vitro embryo production (IVP), specifically because oocyte competence is one of the most important aspects determining the success of IVP. Imprinted genes, such as IGF2, play important roles in embryo development, placentation and fetal growth. The aim of this study was to characterize the DNA methylation profile of the CpG island located in IGF2 exon 10 in oocytes during bovine folliculogenesis. The methylation percentages in oocytes from primordial follicles, final secondary follicles, small antral follicles, large antral follicles, MII oocytes and spermatozoa were 73.74 ± 2.88%, 58.70 ± 7.46%, 56.00 ± 5.58%, 65.77 ± 5.10%, 56.35 ± 7.45% and 96.04 ± 0.78%, respectively. Oocytes from primordial follicles showed fewer hypomethylated alleles (15.5%) than MII oocytes (34.6%) (p = 0.039); spermatozoa showed only hypermethylated alleles. Moreover, MII oocytes were less methylated than spermatozoa (p<0.001). Our results showed that the methylation pattern of this region behaves differently between mature oocytes and spermatozoa. However, while this region has a classical imprinted pattern in spermatozoa that is fully methylated, it was variable in mature oocytes, showing hypermethylated and hypomethylated alleles. Furthermore, our results suggest that this CpG island may have received precocious reprogramming, considering that the hypermethylated pattern was already found in growing oocytes from primordial follicles. These results may contribute to our understanding of the reprogramming of imprinted genes during bovine oogenesis. Public Library of Science 2015-10-30 /pmc/articles/PMC4627647/ /pubmed/26517264 http://dx.doi.org/10.1371/journal.pone.0142072 Text en © 2015 Mendonça et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Mendonça, Anelise dos Santos Guimarães, Ana Luíza Silva da Silva, Naiara Milagres Augusto Caetano, Alexandre Rodrigues Dode, Margot Alves Nunes Franco, Maurício Machaim Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis |
title | Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis |
title_full | Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis |
title_fullStr | Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis |
title_full_unstemmed | Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis |
title_short | Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis |
title_sort | characterization of the igf2 imprinted gene methylation status in bovine oocytes during folliculogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627647/ https://www.ncbi.nlm.nih.gov/pubmed/26517264 http://dx.doi.org/10.1371/journal.pone.0142072 |
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