Cargando…

Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis

DNA methylation reprogramming occurs during mammalian gametogenesis and embryogenesis. Sex-specific DNA methylation patterns at specific CpG islands controlling imprinted genes are acquired during this window of development. Characterization of the DNA methylation dynamics of imprinted genes acquire...

Descripción completa

Detalles Bibliográficos
Autores principales: Mendonça, Anelise dos Santos, Guimarães, Ana Luíza Silva, da Silva, Naiara Milagres Augusto, Caetano, Alexandre Rodrigues, Dode, Margot Alves Nunes, Franco, Maurício Machaim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627647/
https://www.ncbi.nlm.nih.gov/pubmed/26517264
http://dx.doi.org/10.1371/journal.pone.0142072
_version_ 1782398309400313856
author Mendonça, Anelise dos Santos
Guimarães, Ana Luíza Silva
da Silva, Naiara Milagres Augusto
Caetano, Alexandre Rodrigues
Dode, Margot Alves Nunes
Franco, Maurício Machaim
author_facet Mendonça, Anelise dos Santos
Guimarães, Ana Luíza Silva
da Silva, Naiara Milagres Augusto
Caetano, Alexandre Rodrigues
Dode, Margot Alves Nunes
Franco, Maurício Machaim
author_sort Mendonça, Anelise dos Santos
collection PubMed
description DNA methylation reprogramming occurs during mammalian gametogenesis and embryogenesis. Sex-specific DNA methylation patterns at specific CpG islands controlling imprinted genes are acquired during this window of development. Characterization of the DNA methylation dynamics of imprinted genes acquired by oocytes during folliculogenesis is essential for understanding the physiological and genetic aspects of female gametogenesis and to determine the parameters for oocyte competence. This knowledge can be used to improve in vitro embryo production (IVP), specifically because oocyte competence is one of the most important aspects determining the success of IVP. Imprinted genes, such as IGF2, play important roles in embryo development, placentation and fetal growth. The aim of this study was to characterize the DNA methylation profile of the CpG island located in IGF2 exon 10 in oocytes during bovine folliculogenesis. The methylation percentages in oocytes from primordial follicles, final secondary follicles, small antral follicles, large antral follicles, MII oocytes and spermatozoa were 73.74 ± 2.88%, 58.70 ± 7.46%, 56.00 ± 5.58%, 65.77 ± 5.10%, 56.35 ± 7.45% and 96.04 ± 0.78%, respectively. Oocytes from primordial follicles showed fewer hypomethylated alleles (15.5%) than MII oocytes (34.6%) (p = 0.039); spermatozoa showed only hypermethylated alleles. Moreover, MII oocytes were less methylated than spermatozoa (p<0.001). Our results showed that the methylation pattern of this region behaves differently between mature oocytes and spermatozoa. However, while this region has a classical imprinted pattern in spermatozoa that is fully methylated, it was variable in mature oocytes, showing hypermethylated and hypomethylated alleles. Furthermore, our results suggest that this CpG island may have received precocious reprogramming, considering that the hypermethylated pattern was already found in growing oocytes from primordial follicles. These results may contribute to our understanding of the reprogramming of imprinted genes during bovine oogenesis.
format Online
Article
Text
id pubmed-4627647
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-46276472015-11-06 Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis Mendonça, Anelise dos Santos Guimarães, Ana Luíza Silva da Silva, Naiara Milagres Augusto Caetano, Alexandre Rodrigues Dode, Margot Alves Nunes Franco, Maurício Machaim PLoS One Research Article DNA methylation reprogramming occurs during mammalian gametogenesis and embryogenesis. Sex-specific DNA methylation patterns at specific CpG islands controlling imprinted genes are acquired during this window of development. Characterization of the DNA methylation dynamics of imprinted genes acquired by oocytes during folliculogenesis is essential for understanding the physiological and genetic aspects of female gametogenesis and to determine the parameters for oocyte competence. This knowledge can be used to improve in vitro embryo production (IVP), specifically because oocyte competence is one of the most important aspects determining the success of IVP. Imprinted genes, such as IGF2, play important roles in embryo development, placentation and fetal growth. The aim of this study was to characterize the DNA methylation profile of the CpG island located in IGF2 exon 10 in oocytes during bovine folliculogenesis. The methylation percentages in oocytes from primordial follicles, final secondary follicles, small antral follicles, large antral follicles, MII oocytes and spermatozoa were 73.74 ± 2.88%, 58.70 ± 7.46%, 56.00 ± 5.58%, 65.77 ± 5.10%, 56.35 ± 7.45% and 96.04 ± 0.78%, respectively. Oocytes from primordial follicles showed fewer hypomethylated alleles (15.5%) than MII oocytes (34.6%) (p = 0.039); spermatozoa showed only hypermethylated alleles. Moreover, MII oocytes were less methylated than spermatozoa (p<0.001). Our results showed that the methylation pattern of this region behaves differently between mature oocytes and spermatozoa. However, while this region has a classical imprinted pattern in spermatozoa that is fully methylated, it was variable in mature oocytes, showing hypermethylated and hypomethylated alleles. Furthermore, our results suggest that this CpG island may have received precocious reprogramming, considering that the hypermethylated pattern was already found in growing oocytes from primordial follicles. These results may contribute to our understanding of the reprogramming of imprinted genes during bovine oogenesis. Public Library of Science 2015-10-30 /pmc/articles/PMC4627647/ /pubmed/26517264 http://dx.doi.org/10.1371/journal.pone.0142072 Text en © 2015 Mendonça et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mendonça, Anelise dos Santos
Guimarães, Ana Luíza Silva
da Silva, Naiara Milagres Augusto
Caetano, Alexandre Rodrigues
Dode, Margot Alves Nunes
Franco, Maurício Machaim
Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis
title Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis
title_full Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis
title_fullStr Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis
title_full_unstemmed Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis
title_short Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis
title_sort characterization of the igf2 imprinted gene methylation status in bovine oocytes during folliculogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627647/
https://www.ncbi.nlm.nih.gov/pubmed/26517264
http://dx.doi.org/10.1371/journal.pone.0142072
work_keys_str_mv AT mendoncaanelisedossantos characterizationoftheigf2imprintedgenemethylationstatusinbovineoocytesduringfolliculogenesis
AT guimaraesanaluizasilva characterizationoftheigf2imprintedgenemethylationstatusinbovineoocytesduringfolliculogenesis
AT dasilvanaiaramilagresaugusto characterizationoftheigf2imprintedgenemethylationstatusinbovineoocytesduringfolliculogenesis
AT caetanoalexandrerodrigues characterizationoftheigf2imprintedgenemethylationstatusinbovineoocytesduringfolliculogenesis
AT dodemargotalvesnunes characterizationoftheigf2imprintedgenemethylationstatusinbovineoocytesduringfolliculogenesis
AT francomauriciomachaim characterizationoftheigf2imprintedgenemethylationstatusinbovineoocytesduringfolliculogenesis