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Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species

BACKGROUND: Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria. Leptospira, unlike other human-pathogenic spirochetes, produce LPS, which is fundamental to the taxonomy of the genus, involved in host-adaption and also...

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Autores principales: Patra, Kailash P., Choudhury, Biswa, Matthias, Michael M., Baga, Sheyenne, Bandyopadhya, Keya, Vinetz, Joseph M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4628369/
https://www.ncbi.nlm.nih.gov/pubmed/26518696
http://dx.doi.org/10.1186/s12866-015-0581-7
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author Patra, Kailash P.
Choudhury, Biswa
Matthias, Michael M.
Baga, Sheyenne
Bandyopadhya, Keya
Vinetz, Joseph M.
author_facet Patra, Kailash P.
Choudhury, Biswa
Matthias, Michael M.
Baga, Sheyenne
Bandyopadhya, Keya
Vinetz, Joseph M.
author_sort Patra, Kailash P.
collection PubMed
description BACKGROUND: Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria. Leptospira, unlike other human-pathogenic spirochetes, produce LPS, which is fundamental to the taxonomy of the genus, involved in host-adaption and also the target of diagnostic antibodies. Despite its significance, little is known of Leptospira LPS composition and carbohydrate structure among different serovars. RESULTS: LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied. LPS prepared from aqueous and phenol phases were analyzed separately. L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7 %), a high amount of GlcNAc (12.3 %), and two different types of dideoxy HexNAc. SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal. Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal. Arabinose, xylose, mannose, galactose and L-glycero-D-mannoheptose were detected in both the species. Fatty acid analysis by gas chromatography–mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni. Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal. CONCLUSIONS: This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
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spelling pubmed-46283692015-11-01 Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species Patra, Kailash P. Choudhury, Biswa Matthias, Michael M. Baga, Sheyenne Bandyopadhya, Keya Vinetz, Joseph M. BMC Microbiol Research Article BACKGROUND: Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria. Leptospira, unlike other human-pathogenic spirochetes, produce LPS, which is fundamental to the taxonomy of the genus, involved in host-adaption and also the target of diagnostic antibodies. Despite its significance, little is known of Leptospira LPS composition and carbohydrate structure among different serovars. RESULTS: LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied. LPS prepared from aqueous and phenol phases were analyzed separately. L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7 %), a high amount of GlcNAc (12.3 %), and two different types of dideoxy HexNAc. SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal. Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal. Arabinose, xylose, mannose, galactose and L-glycero-D-mannoheptose were detected in both the species. Fatty acid analysis by gas chromatography–mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni. Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal. CONCLUSIONS: This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis. BioMed Central 2015-10-30 /pmc/articles/PMC4628369/ /pubmed/26518696 http://dx.doi.org/10.1186/s12866-015-0581-7 Text en © Patra et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Patra, Kailash P.
Choudhury, Biswa
Matthias, Michael M.
Baga, Sheyenne
Bandyopadhya, Keya
Vinetz, Joseph M.
Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species
title Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species
title_full Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species
title_fullStr Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species
title_full_unstemmed Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species
title_short Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species
title_sort comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic leptospira species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4628369/
https://www.ncbi.nlm.nih.gov/pubmed/26518696
http://dx.doi.org/10.1186/s12866-015-0581-7
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