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Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum

The purpose is to study the intervention, proliferation, and differentiation on fibroblast by Shuizhongcao Granule during the treatment of ROU and investigate the action mechanism in inflammatory microenvironment. Proliferation of rat fibroblasts was detected using CCK8. Western blot was used to det...

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Autores principales: Bo, Zhang, Xiang, Qian, Shan-ming, Ruan, Wang, Bei, De-hou, Deng, Liang, Xia, Qing-lin, Li, Feng, Tao, Min-he, Shen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4628681/
https://www.ncbi.nlm.nih.gov/pubmed/26557145
http://dx.doi.org/10.1155/2015/324091
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author Bo, Zhang
Xiang, Qian
Shan-ming, Ruan
Wang, Bei
De-hou, Deng
Liang, Xia
Qing-lin, Li
Feng, Tao
Min-he, Shen
author_facet Bo, Zhang
Xiang, Qian
Shan-ming, Ruan
Wang, Bei
De-hou, Deng
Liang, Xia
Qing-lin, Li
Feng, Tao
Min-he, Shen
author_sort Bo, Zhang
collection PubMed
description The purpose is to study the intervention, proliferation, and differentiation on fibroblast by Shuizhongcao Granule during the treatment of ROU and investigate the action mechanism in inflammatory microenvironment. Proliferation of rat fibroblasts was detected using CCK8. Western blot was used to detect the effect of drug-containing serum on the expression of protein associated with NF-κB and ERK pathway in rat fibroblasts. Expression of IL-10 and IL-12 was detected by PCR. Shuizhongcao Granule group successfully inhibited proliferation of rat fibroblast. Western blot results revealed that p65 and IKKB were significantly less expressed in Chinese medicine group, while pIκBα and pIKKαβ expression were significantly increased. We have also found that in this group the expression of pAKT was evidently suppressed and expression of pERK significantly decreased. PCR results showed significantly decreased expression levels of IL-10 and 1IL-12b in Chinese medicine group, while the expression of IL-12a was increased. Our results suggest that Shuizhongcao Granule can suppress the proliferation of fibroblast and inhibit the activation of NF-κB and thus suppress inflammatory reactions, possibly involving the inhibited expression of phosphorylated AKT, rather than the canonical pathway. Furthermore, it can inhibit ERK pathway and reduce IL-10 and IL-12b gene expression while enhancing IL-12a expression.
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spelling pubmed-46286812015-11-09 Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum Bo, Zhang Xiang, Qian Shan-ming, Ruan Wang, Bei De-hou, Deng Liang, Xia Qing-lin, Li Feng, Tao Min-he, Shen Evid Based Complement Alternat Med Research Article The purpose is to study the intervention, proliferation, and differentiation on fibroblast by Shuizhongcao Granule during the treatment of ROU and investigate the action mechanism in inflammatory microenvironment. Proliferation of rat fibroblasts was detected using CCK8. Western blot was used to detect the effect of drug-containing serum on the expression of protein associated with NF-κB and ERK pathway in rat fibroblasts. Expression of IL-10 and IL-12 was detected by PCR. Shuizhongcao Granule group successfully inhibited proliferation of rat fibroblast. Western blot results revealed that p65 and IKKB were significantly less expressed in Chinese medicine group, while pIκBα and pIKKαβ expression were significantly increased. We have also found that in this group the expression of pAKT was evidently suppressed and expression of pERK significantly decreased. PCR results showed significantly decreased expression levels of IL-10 and 1IL-12b in Chinese medicine group, while the expression of IL-12a was increased. Our results suggest that Shuizhongcao Granule can suppress the proliferation of fibroblast and inhibit the activation of NF-κB and thus suppress inflammatory reactions, possibly involving the inhibited expression of phosphorylated AKT, rather than the canonical pathway. Furthermore, it can inhibit ERK pathway and reduce IL-10 and IL-12b gene expression while enhancing IL-12a expression. Hindawi Publishing Corporation 2015 2015-10-18 /pmc/articles/PMC4628681/ /pubmed/26557145 http://dx.doi.org/10.1155/2015/324091 Text en Copyright © 2015 Zhang Bo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bo, Zhang
Xiang, Qian
Shan-ming, Ruan
Wang, Bei
De-hou, Deng
Liang, Xia
Qing-lin, Li
Feng, Tao
Min-he, Shen
Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum
title Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum
title_full Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum
title_fullStr Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum
title_full_unstemmed Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum
title_short Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum
title_sort investigation on molecular mechanism of fibroblast regulation and the treatment of recurrent oral ulcer by shuizhongcao granule-containing serum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4628681/
https://www.ncbi.nlm.nih.gov/pubmed/26557145
http://dx.doi.org/10.1155/2015/324091
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