DESI–MS as a tool for direct lipid analysis in cultured cells

Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addr...

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Autores principales: Bodzon-Kulakowska, Anna, Cichon, Tomasz, Golec, Agnieszka, Drabik, Anna, Ner, Joanna, Suder, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2014
Materias:
Method in Cell Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4628929/
https://www.ncbi.nlm.nih.gov/pubmed/24801580
http://dx.doi.org/10.1007/s10616-014-9734-z
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author Bodzon-Kulakowska, Anna
Cichon, Tomasz
Golec, Agnieszka
Drabik, Anna
Ner, Joanna
Suder, Piotr
author_facet Bodzon-Kulakowska, Anna
Cichon, Tomasz
Golec, Agnieszka
Drabik, Anna
Ner, Joanna
Suder, Piotr
author_sort Bodzon-Kulakowska, Anna
collection PubMed
description Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface preparation of cell culture slides, induction of oxidative stress, and cell monolayer culture preparation as well as optimization of the analysis. Advantages and drawbacks of the method were also discussed.
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spelling pubmed-46289292015-11-06 DESI–MS as a tool for direct lipid analysis in cultured cells Bodzon-Kulakowska, Anna Cichon, Tomasz Golec, Agnieszka Drabik, Anna Ner, Joanna Suder, Piotr Cytotechnology Method in Cell Science Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface preparation of cell culture slides, induction of oxidative stress, and cell monolayer culture preparation as well as optimization of the analysis. Advantages and drawbacks of the method were also discussed. Springer Netherlands 2014-05-07 2015-12 /pmc/articles/PMC4628929/ /pubmed/24801580 http://dx.doi.org/10.1007/s10616-014-9734-z Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Method in Cell Science
Bodzon-Kulakowska, Anna
Cichon, Tomasz
Golec, Agnieszka
Drabik, Anna
Ner, Joanna
Suder, Piotr
DESI–MS as a tool for direct lipid analysis in cultured cells
title DESI–MS as a tool for direct lipid analysis in cultured cells
title_full DESI–MS as a tool for direct lipid analysis in cultured cells
title_fullStr DESI–MS as a tool for direct lipid analysis in cultured cells
title_full_unstemmed DESI–MS as a tool for direct lipid analysis in cultured cells
title_short DESI–MS as a tool for direct lipid analysis in cultured cells
title_sort desi–ms as a tool for direct lipid analysis in cultured cells
topic Method in Cell Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4628929/
https://www.ncbi.nlm.nih.gov/pubmed/24801580
http://dx.doi.org/10.1007/s10616-014-9734-z
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