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Commercialized non-Camellia tea: traditional function and molecular identification
Non-Camellia tea is a part of the colorful Chinese tea culture, and is also widely used as beverage and medicine in folk for disease prevention and treatment. In this study, 37 samples were collected, including 33 kinds of non-Camellia teas and 4 kinds of teas (Camellia). Traditional functions of no...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629065/ https://www.ncbi.nlm.nih.gov/pubmed/26579387 http://dx.doi.org/10.1016/j.apsb.2014.02.006 |
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author | Long, Ping Cui, Zhanhu Wang, Yingli Zhang, Chunhong Zhang, Na Li, Minhui Xiao, Peigen |
author_facet | Long, Ping Cui, Zhanhu Wang, Yingli Zhang, Chunhong Zhang, Na Li, Minhui Xiao, Peigen |
author_sort | Long, Ping |
collection | PubMed |
description | Non-Camellia tea is a part of the colorful Chinese tea culture, and is also widely used as beverage and medicine in folk for disease prevention and treatment. In this study, 37 samples were collected, including 33 kinds of non-Camellia teas and 4 kinds of teas (Camellia). Traditional functions of non-Camellia teas were investigated. Furthermore, non-Camellia teas of original plants were characterized and identified by molecular methods. Four candidate regions (rbcL, matK, ITS2, psbA-trnH) were amplified by polymerase chain reaction. In addition, DNA barcodes were used for the first time to discriminate the commercial non-Camellia tea and their adulterants, and to evaluate their safety. This study showed that BLASTN and the relevant phylogenetic tree are efficient tools for identification of the commercial non-Camellia tea and their adulterants. However, some sequences from original plants have not been found and there is a limitation of sequence number of original plants in GenBank. Submitting more original plant sequences to the GenBank will be helpful for evaluating the safety of non-Camellia teas. |
format | Online Article Text |
id | pubmed-4629065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-46290652015-11-17 Commercialized non-Camellia tea: traditional function and molecular identification Long, Ping Cui, Zhanhu Wang, Yingli Zhang, Chunhong Zhang, Na Li, Minhui Xiao, Peigen Acta Pharm Sin B Original Article Non-Camellia tea is a part of the colorful Chinese tea culture, and is also widely used as beverage and medicine in folk for disease prevention and treatment. In this study, 37 samples were collected, including 33 kinds of non-Camellia teas and 4 kinds of teas (Camellia). Traditional functions of non-Camellia teas were investigated. Furthermore, non-Camellia teas of original plants were characterized and identified by molecular methods. Four candidate regions (rbcL, matK, ITS2, psbA-trnH) were amplified by polymerase chain reaction. In addition, DNA barcodes were used for the first time to discriminate the commercial non-Camellia tea and their adulterants, and to evaluate their safety. This study showed that BLASTN and the relevant phylogenetic tree are efficient tools for identification of the commercial non-Camellia tea and their adulterants. However, some sequences from original plants have not been found and there is a limitation of sequence number of original plants in GenBank. Submitting more original plant sequences to the GenBank will be helpful for evaluating the safety of non-Camellia teas. Elsevier 2014-06 2014-03-24 /pmc/articles/PMC4629065/ /pubmed/26579387 http://dx.doi.org/10.1016/j.apsb.2014.02.006 Text en © 2014 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Original Article Long, Ping Cui, Zhanhu Wang, Yingli Zhang, Chunhong Zhang, Na Li, Minhui Xiao, Peigen Commercialized non-Camellia tea: traditional function and molecular identification |
title | Commercialized non-Camellia tea: traditional function and molecular identification |
title_full | Commercialized non-Camellia tea: traditional function and molecular identification |
title_fullStr | Commercialized non-Camellia tea: traditional function and molecular identification |
title_full_unstemmed | Commercialized non-Camellia tea: traditional function and molecular identification |
title_short | Commercialized non-Camellia tea: traditional function and molecular identification |
title_sort | commercialized non-camellia tea: traditional function and molecular identification |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629065/ https://www.ncbi.nlm.nih.gov/pubmed/26579387 http://dx.doi.org/10.1016/j.apsb.2014.02.006 |
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