Cargando…
Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity
Amyloid beta-peptides (Aβ) are known to undergo active transport across the blood-brain barrier, and cerebral amyloid angiopathy has been shown to be a prominent feature in the majority of Alzheimer׳s disease. Quercetin is a natural flavonoid molecule and has been demonstrated to have potent neuropr...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629123/ https://www.ncbi.nlm.nih.gov/pubmed/26579424 http://dx.doi.org/10.1016/j.apsb.2014.12.003 |
_version_ | 1782398533370904576 |
---|---|
author | Li, Yongjie Zhou, Sibai Li, Jinze Sun, Yuhua Hasimu, Hamlati Liu, Rui Zhang, Tiantai |
author_facet | Li, Yongjie Zhou, Sibai Li, Jinze Sun, Yuhua Hasimu, Hamlati Liu, Rui Zhang, Tiantai |
author_sort | Li, Yongjie |
collection | PubMed |
description | Amyloid beta-peptides (Aβ) are known to undergo active transport across the blood-brain barrier, and cerebral amyloid angiopathy has been shown to be a prominent feature in the majority of Alzheimer׳s disease. Quercetin is a natural flavonoid molecule and has been demonstrated to have potent neuroprotective effects, but its protective effect on endothelial cells under Aβ-damaged condition is unclear. In the present study, the protective effects of quercetin on brain microvascular endothelial cells injured by fibrillar Aβ(1–40) (fAβ(1–40)) were observed. The results show that fAβ(1–40)-induced cytotoxicity in human brain microvascular endothelial cells (hBMECs) can be relieved by quercetin treatment. Quercetin increases cell viability, reduces the release of lactate dehydrogenase, and relieves nuclear condensation. Quercetin also alleviates intracellular reactive oxygen species generation and increases superoxide dismutase activity. Moreover, it strengthens the barrier integrity through the preservation of the transendothelial electrical resistance value, the relief of aggravated permeability, and the increase of characteristic enzyme levels after being exposed to fAβ(1–40). In conclusion, quercetin protects hBMECs from fAβ(1–40)-induced toxicity. |
format | Online Article Text |
id | pubmed-4629123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-46291232015-11-17 Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity Li, Yongjie Zhou, Sibai Li, Jinze Sun, Yuhua Hasimu, Hamlati Liu, Rui Zhang, Tiantai Acta Pharm Sin B Original Article Amyloid beta-peptides (Aβ) are known to undergo active transport across the blood-brain barrier, and cerebral amyloid angiopathy has been shown to be a prominent feature in the majority of Alzheimer׳s disease. Quercetin is a natural flavonoid molecule and has been demonstrated to have potent neuroprotective effects, but its protective effect on endothelial cells under Aβ-damaged condition is unclear. In the present study, the protective effects of quercetin on brain microvascular endothelial cells injured by fibrillar Aβ(1–40) (fAβ(1–40)) were observed. The results show that fAβ(1–40)-induced cytotoxicity in human brain microvascular endothelial cells (hBMECs) can be relieved by quercetin treatment. Quercetin increases cell viability, reduces the release of lactate dehydrogenase, and relieves nuclear condensation. Quercetin also alleviates intracellular reactive oxygen species generation and increases superoxide dismutase activity. Moreover, it strengthens the barrier integrity through the preservation of the transendothelial electrical resistance value, the relief of aggravated permeability, and the increase of characteristic enzyme levels after being exposed to fAβ(1–40). In conclusion, quercetin protects hBMECs from fAβ(1–40)-induced toxicity. Elsevier 2015-01 2015-01-13 /pmc/articles/PMC4629123/ /pubmed/26579424 http://dx.doi.org/10.1016/j.apsb.2014.12.003 Text en © 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Li, Yongjie Zhou, Sibai Li, Jinze Sun, Yuhua Hasimu, Hamlati Liu, Rui Zhang, Tiantai Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity |
title | Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity |
title_full | Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity |
title_fullStr | Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity |
title_full_unstemmed | Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity |
title_short | Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity |
title_sort | quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629123/ https://www.ncbi.nlm.nih.gov/pubmed/26579424 http://dx.doi.org/10.1016/j.apsb.2014.12.003 |
work_keys_str_mv | AT liyongjie quercetinprotectshumanbrainmicrovascularendothelialcellsfromfibrillarbamyloid140inducedtoxicity AT zhousibai quercetinprotectshumanbrainmicrovascularendothelialcellsfromfibrillarbamyloid140inducedtoxicity AT lijinze quercetinprotectshumanbrainmicrovascularendothelialcellsfromfibrillarbamyloid140inducedtoxicity AT sunyuhua quercetinprotectshumanbrainmicrovascularendothelialcellsfromfibrillarbamyloid140inducedtoxicity AT hasimuhamlati quercetinprotectshumanbrainmicrovascularendothelialcellsfromfibrillarbamyloid140inducedtoxicity AT liurui quercetinprotectshumanbrainmicrovascularendothelialcellsfromfibrillarbamyloid140inducedtoxicity AT zhangtiantai quercetinprotectshumanbrainmicrovascularendothelialcellsfromfibrillarbamyloid140inducedtoxicity |