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Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity

Amyloid beta-peptides (Aβ) are known to undergo active transport across the blood-brain barrier, and cerebral amyloid angiopathy has been shown to be a prominent feature in the majority of Alzheimer׳s disease. Quercetin is a natural flavonoid molecule and has been demonstrated to have potent neuropr...

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Autores principales: Li, Yongjie, Zhou, Sibai, Li, Jinze, Sun, Yuhua, Hasimu, Hamlati, Liu, Rui, Zhang, Tiantai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629123/
https://www.ncbi.nlm.nih.gov/pubmed/26579424
http://dx.doi.org/10.1016/j.apsb.2014.12.003
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author Li, Yongjie
Zhou, Sibai
Li, Jinze
Sun, Yuhua
Hasimu, Hamlati
Liu, Rui
Zhang, Tiantai
author_facet Li, Yongjie
Zhou, Sibai
Li, Jinze
Sun, Yuhua
Hasimu, Hamlati
Liu, Rui
Zhang, Tiantai
author_sort Li, Yongjie
collection PubMed
description Amyloid beta-peptides (Aβ) are known to undergo active transport across the blood-brain barrier, and cerebral amyloid angiopathy has been shown to be a prominent feature in the majority of Alzheimer׳s disease. Quercetin is a natural flavonoid molecule and has been demonstrated to have potent neuroprotective effects, but its protective effect on endothelial cells under Aβ-damaged condition is unclear. In the present study, the protective effects of quercetin on brain microvascular endothelial cells injured by fibrillar Aβ(1–40) (fAβ(1–40)) were observed. The results show that fAβ(1–40)-induced cytotoxicity in human brain microvascular endothelial cells (hBMECs) can be relieved by quercetin treatment. Quercetin increases cell viability, reduces the release of lactate dehydrogenase, and relieves nuclear condensation. Quercetin also alleviates intracellular reactive oxygen species generation and increases superoxide dismutase activity. Moreover, it strengthens the barrier integrity through the preservation of the transendothelial electrical resistance value, the relief of aggravated permeability, and the increase of characteristic enzyme levels after being exposed to fAβ(1–40). In conclusion, quercetin protects hBMECs from fAβ(1–40)-induced toxicity.
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spelling pubmed-46291232015-11-17 Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity Li, Yongjie Zhou, Sibai Li, Jinze Sun, Yuhua Hasimu, Hamlati Liu, Rui Zhang, Tiantai Acta Pharm Sin B Original Article Amyloid beta-peptides (Aβ) are known to undergo active transport across the blood-brain barrier, and cerebral amyloid angiopathy has been shown to be a prominent feature in the majority of Alzheimer׳s disease. Quercetin is a natural flavonoid molecule and has been demonstrated to have potent neuroprotective effects, but its protective effect on endothelial cells under Aβ-damaged condition is unclear. In the present study, the protective effects of quercetin on brain microvascular endothelial cells injured by fibrillar Aβ(1–40) (fAβ(1–40)) were observed. The results show that fAβ(1–40)-induced cytotoxicity in human brain microvascular endothelial cells (hBMECs) can be relieved by quercetin treatment. Quercetin increases cell viability, reduces the release of lactate dehydrogenase, and relieves nuclear condensation. Quercetin also alleviates intracellular reactive oxygen species generation and increases superoxide dismutase activity. Moreover, it strengthens the barrier integrity through the preservation of the transendothelial electrical resistance value, the relief of aggravated permeability, and the increase of characteristic enzyme levels after being exposed to fAβ(1–40). In conclusion, quercetin protects hBMECs from fAβ(1–40)-induced toxicity. Elsevier 2015-01 2015-01-13 /pmc/articles/PMC4629123/ /pubmed/26579424 http://dx.doi.org/10.1016/j.apsb.2014.12.003 Text en © 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Li, Yongjie
Zhou, Sibai
Li, Jinze
Sun, Yuhua
Hasimu, Hamlati
Liu, Rui
Zhang, Tiantai
Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity
title Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity
title_full Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity
title_fullStr Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity
title_full_unstemmed Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity
title_short Quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity
title_sort quercetin protects human brain microvascular endothelial cells from fibrillar β-amyloid(1–40)-induced toxicity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629123/
https://www.ncbi.nlm.nih.gov/pubmed/26579424
http://dx.doi.org/10.1016/j.apsb.2014.12.003
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