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Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats

The purpose of this study is to investigate the expression of major potassium channel subtypes in the brain of chronical mild stress (CMS) rats and reveal the effects of fluoxetine on the expression of these channels. Rats were exposed to a variety of unpredictable stress for three weeks and induced...

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Autores principales: Chen, Chunlin, Wang, Ling, Rong, Xianfang, Wang, Weiping, Wang, Xiaoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629207/
https://www.ncbi.nlm.nih.gov/pubmed/26579425
http://dx.doi.org/10.1016/j.apsb.2014.12.004
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author Chen, Chunlin
Wang, Ling
Rong, Xianfang
Wang, Weiping
Wang, Xiaoliang
author_facet Chen, Chunlin
Wang, Ling
Rong, Xianfang
Wang, Weiping
Wang, Xiaoliang
author_sort Chen, Chunlin
collection PubMed
description The purpose of this study is to investigate the expression of major potassium channel subtypes in the brain of chronical mild stress (CMS) rats and reveal the effects of fluoxetine on the expression of these channels. Rats were exposed to a variety of unpredictable stress for three weeks and induced anhedonia, lower sucrose preference, locomotor activity and lower body weight. The protein expressions were determined by Western blot. CMS significantly increased the expression of Kv2.1 channel in frontal cortex but not in hippocampus, and the expression level was normalized after fluoxetine treatment. The expression of TREK-1 channel was also obviously increased in frontal cortex in CMS rats. Fluoxetine treatment might prevent this increase. However, the expression of Kv3.1 and Kv4.2 channels was considerably decreased in hippocampus after CMS, and was not affected by fluoxetine. These results suggest that different subtypes of potassium channels are associated with the pathophysiology of depression and that the therapeutical effects of fluoxetine may relate to Kv2.1 and TREK-1 potassium channels.
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spelling pubmed-46292072015-11-17 Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats Chen, Chunlin Wang, Ling Rong, Xianfang Wang, Weiping Wang, Xiaoliang Acta Pharm Sin B Original Article The purpose of this study is to investigate the expression of major potassium channel subtypes in the brain of chronical mild stress (CMS) rats and reveal the effects of fluoxetine on the expression of these channels. Rats were exposed to a variety of unpredictable stress for three weeks and induced anhedonia, lower sucrose preference, locomotor activity and lower body weight. The protein expressions were determined by Western blot. CMS significantly increased the expression of Kv2.1 channel in frontal cortex but not in hippocampus, and the expression level was normalized after fluoxetine treatment. The expression of TREK-1 channel was also obviously increased in frontal cortex in CMS rats. Fluoxetine treatment might prevent this increase. However, the expression of Kv3.1 and Kv4.2 channels was considerably decreased in hippocampus after CMS, and was not affected by fluoxetine. These results suggest that different subtypes of potassium channels are associated with the pathophysiology of depression and that the therapeutical effects of fluoxetine may relate to Kv2.1 and TREK-1 potassium channels. Elsevier 2015-01 2014-12-30 /pmc/articles/PMC4629207/ /pubmed/26579425 http://dx.doi.org/10.1016/j.apsb.2014.12.004 Text en © 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Chen, Chunlin
Wang, Ling
Rong, Xianfang
Wang, Weiping
Wang, Xiaoliang
Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats
title Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats
title_full Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats
title_fullStr Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats
title_full_unstemmed Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats
title_short Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats
title_sort effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629207/
https://www.ncbi.nlm.nih.gov/pubmed/26579425
http://dx.doi.org/10.1016/j.apsb.2014.12.004
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