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Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts

Ginsenoside compound K (GCK), the main metabolite of protopanaxadiol constituents of Panax ginseng, easily produces alkali metal adduct ions during mass spectrometry particularly with lithium. Accordingly, we have developed a rapid and sensitive liquid chromatography–tandem mass spectrometric method...

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Detalles Bibliográficos
Autores principales: Chen, Yunhui, Lu, Youming, Yang, Yong, Chen, Xiaoyan, Zhu, Liang, Zhong, Dafang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629438/
https://www.ncbi.nlm.nih.gov/pubmed/26579476
http://dx.doi.org/10.1016/j.apsb.2015.06.003
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author Chen, Yunhui
Lu, Youming
Yang, Yong
Chen, Xiaoyan
Zhu, Liang
Zhong, Dafang
author_facet Chen, Yunhui
Lu, Youming
Yang, Yong
Chen, Xiaoyan
Zhu, Liang
Zhong, Dafang
author_sort Chen, Yunhui
collection PubMed
description Ginsenoside compound K (GCK), the main metabolite of protopanaxadiol constituents of Panax ginseng, easily produces alkali metal adduct ions during mass spectrometry particularly with lithium. Accordingly, we have developed a rapid and sensitive liquid chromatography–tandem mass spectrometric method for analysis of GCK in human plasma based on formation of a lithium adduct. The analyte and paclitaxel (internal standard) were extracted from 50 µL human plasma using methyl tert-butyl ether. Chromatographic separation was performed on a Phenomenex Gemini C18 column (50 mm×2.0 mm; 5 μm) using stepwise gradient elution with acetonitrile–water and 0.2 mmol/L lithium carbonate at a flow rate of 0.5 mL/min. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions at m/z 629→449 for the GCK-lithium adduct and m/z 860→292 for the adduct of paclitaxel. The assay was linear in the concentration range 1.00–1000 ng/mL (r(2)>0.9988) with intra- and inter-day precision of ±8.4% and accuracy in the range of −4.8% to 6.5%. Recovery, stability and matrix effects were all satisfactory. The method was successfully applied to a pharmacokinetic study involving administration of a single GCK 50 mg tablet to healthy Chinese volunteers.
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spelling pubmed-46294382015-11-17 Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts Chen, Yunhui Lu, Youming Yang, Yong Chen, Xiaoyan Zhu, Liang Zhong, Dafang Acta Pharm Sin B Original Article Ginsenoside compound K (GCK), the main metabolite of protopanaxadiol constituents of Panax ginseng, easily produces alkali metal adduct ions during mass spectrometry particularly with lithium. Accordingly, we have developed a rapid and sensitive liquid chromatography–tandem mass spectrometric method for analysis of GCK in human plasma based on formation of a lithium adduct. The analyte and paclitaxel (internal standard) were extracted from 50 µL human plasma using methyl tert-butyl ether. Chromatographic separation was performed on a Phenomenex Gemini C18 column (50 mm×2.0 mm; 5 μm) using stepwise gradient elution with acetonitrile–water and 0.2 mmol/L lithium carbonate at a flow rate of 0.5 mL/min. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions at m/z 629→449 for the GCK-lithium adduct and m/z 860→292 for the adduct of paclitaxel. The assay was linear in the concentration range 1.00–1000 ng/mL (r(2)>0.9988) with intra- and inter-day precision of ±8.4% and accuracy in the range of −4.8% to 6.5%. Recovery, stability and matrix effects were all satisfactory. The method was successfully applied to a pharmacokinetic study involving administration of a single GCK 50 mg tablet to healthy Chinese volunteers. Elsevier 2015-09 2015-08-19 /pmc/articles/PMC4629438/ /pubmed/26579476 http://dx.doi.org/10.1016/j.apsb.2015.06.003 Text en © 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Chen, Yunhui
Lu, Youming
Yang, Yong
Chen, Xiaoyan
Zhu, Liang
Zhong, Dafang
Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts
title Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts
title_full Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts
title_fullStr Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts
title_full_unstemmed Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts
title_short Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts
title_sort determination of ginsenoside compound k in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629438/
https://www.ncbi.nlm.nih.gov/pubmed/26579476
http://dx.doi.org/10.1016/j.apsb.2015.06.003
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