Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan

This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterho...

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Autores principales: Tigani-Asil, A.E. El, Sanousi, S.M. EL, Aljameel, M.A., Beir, H. El, Adam, A., Abdallatif, M.M., Hamid, M.E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629588/
https://www.ncbi.nlm.nih.gov/pubmed/26623334
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author Tigani-Asil, A.E. El
Sanousi, S.M. EL
Aljameel, M.A.
Beir, H. El
Adam, A.
Abdallatif, M.M.
Hamid, M.E.
author_facet Tigani-Asil, A.E. El
Sanousi, S.M. EL
Aljameel, M.A.
Beir, H. El
Adam, A.
Abdallatif, M.M.
Hamid, M.E.
author_sort Tigani-Asil, A.E. El
collection PubMed
description This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated.
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spelling pubmed-46295882015-11-30 Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan Tigani-Asil, A.E. El Sanousi, S.M. EL Aljameel, M.A. Beir, H. El Adam, A. Abdallatif, M.M. Hamid, M.E. Open Vet J Original Article This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated. Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2014 2014-02-25 /pmc/articles/PMC4629588/ /pubmed/26623334 Text en Copyright: © Open Veterinary Journal http://creativecommons.org/licenses/by/4.0/ Open Veterinary Journal is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Original Article
Tigani-Asil, A.E. El
Sanousi, S.M. EL
Aljameel, M.A.
Beir, H. El
Adam, A.
Abdallatif, M.M.
Hamid, M.E.
Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_full Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_fullStr Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_full_unstemmed Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_short Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_sort molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in south darfur state and alsabalouga slaughterhouse at omdurman area, sudan
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629588/
https://www.ncbi.nlm.nih.gov/pubmed/26623334
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