Ultrasensitive enzyme-linked immunosorbent assay (ELISA) of proteins by combination with the thio-NAD cycling method

An ultrasensitive method for the determination of proteins is described that combines an enzyme-linked immunosorbent assay (ELISA) and a thionicotinamide-adenine dinucleotide (thio-NAD) cycling method. A sandwich method using a primary and a secondary antibody for antigens is employed in an ELISA. A...

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Detalles Bibliográficos
Autores principales: Watabe, Satoshi, Kodama, Hiromi, Kaneda, Mugiho, Morikawa, Mika, Nakaishi, Kazunari, Yoshimura, Teruki, Iwai, Atsushi, Miura, Toshiaki, Ito, Etsuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan (BSJ) 2014
Materias:
Experimental Methods and Protocols
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629663/
https://www.ncbi.nlm.nih.gov/pubmed/27493498
http://dx.doi.org/10.2142/biophysics.10.49

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629663/
https://www.ncbi.nlm.nih.gov/pubmed/27493498
http://dx.doi.org/10.2142/biophysics.10.49

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