Reconstitution of intracellular environments in vitro and in artificial cells

Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomac...

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Detalles Bibliográficos
Autores principales: Fujiwara, Kei, Yanagisawa, Miho, Nomura, Shin-ichiro M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan (BSJ) 2014
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629665/
https://www.ncbi.nlm.nih.gov/pubmed/27493497
http://dx.doi.org/10.2142/biophysics.10.43
Descripción
Sumario:Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomacromolecules in artificial cells. In this review, we introduce our recent progress to reconstitute intracellular environments in vitro and in artificial cells.

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