Reconstitution of intracellular environments in vitro and in artificial cells

Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomac...

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Detalles Bibliográficos
Autores principales: Fujiwara, Kei, Yanagisawa, Miho, Nomura, Shin-ichiro M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan (BSJ) 2014
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629665/
https://www.ncbi.nlm.nih.gov/pubmed/27493497
http://dx.doi.org/10.2142/biophysics.10.43
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author Fujiwara, Kei
Yanagisawa, Miho
Nomura, Shin-ichiro M.
author_facet Fujiwara, Kei
Yanagisawa, Miho
Nomura, Shin-ichiro M.
author_sort Fujiwara, Kei
collection PubMed
description Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomacromolecules in artificial cells. In this review, we introduce our recent progress to reconstitute intracellular environments in vitro and in artificial cells.
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spelling pubmed-46296652016-08-04 Reconstitution of intracellular environments in vitro and in artificial cells Fujiwara, Kei Yanagisawa, Miho Nomura, Shin-ichiro M. Biophysics (Nagoya-shi) Review Article Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomacromolecules in artificial cells. In this review, we introduce our recent progress to reconstitute intracellular environments in vitro and in artificial cells. The Biophysical Society of Japan (BSJ) 2014-08-07 /pmc/articles/PMC4629665/ /pubmed/27493497 http://dx.doi.org/10.2142/biophysics.10.43 Text en ©2014 THE BIOPHYSICAL SOCIETY OF JAPAN
spellingShingle Review Article
Fujiwara, Kei
Yanagisawa, Miho
Nomura, Shin-ichiro M.
Reconstitution of intracellular environments in vitro and in artificial cells
title Reconstitution of intracellular environments in vitro and in artificial cells
title_full Reconstitution of intracellular environments in vitro and in artificial cells
title_fullStr Reconstitution of intracellular environments in vitro and in artificial cells
title_full_unstemmed Reconstitution of intracellular environments in vitro and in artificial cells
title_short Reconstitution of intracellular environments in vitro and in artificial cells
title_sort reconstitution of intracellular environments in vitro and in artificial cells
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629665/
https://www.ncbi.nlm.nih.gov/pubmed/27493497
http://dx.doi.org/10.2142/biophysics.10.43
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AT yanagisawamiho reconstitutionofintracellularenvironmentsinvitroandinartificialcells
AT nomurashinichirom reconstitutionofintracellularenvironmentsinvitroandinartificialcells

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