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3D structure of eukaryotic flagella/cilia by cryo-electron tomography

Flagella/cilia are motile organelles with more than 400 proteins. To understand the mechanism of such complex systems, we need methods to describe molecular arrange-ments and conformations three-dimensionally in vivo. Cryo-electron tomography enabled us such a 3D structural analysis. Our group has b...

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Detalles Bibliográficos
Autor principal: Ishikawa, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan (BSJ) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629670/
https://www.ncbi.nlm.nih.gov/pubmed/27493552
http://dx.doi.org/10.2142/biophysics.9.141
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author Ishikawa, Takashi
author_facet Ishikawa, Takashi
author_sort Ishikawa, Takashi
collection PubMed
description Flagella/cilia are motile organelles with more than 400 proteins. To understand the mechanism of such complex systems, we need methods to describe molecular arrange-ments and conformations three-dimensionally in vivo. Cryo-electron tomography enabled us such a 3D structural analysis. Our group has been working on 3D structure of flagella/cilia using this method and revealed highly ordered and beautifully organized molecular arrangement. 3D structure gave us insights into the mechanism to gener-ate bending motion with well defined waveforms. In this review, I summarize our recent structural studies on fla-gella/cilia by cryo-electron tomography, mainly focusing on dynein microtubule-based ATPase motor proteins and the radial spoke, a regulatory protein complex.
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spelling pubmed-46296702016-08-04 3D structure of eukaryotic flagella/cilia by cryo-electron tomography Ishikawa, Takashi Biophysics (Nagoya-shi) Review Article Flagella/cilia are motile organelles with more than 400 proteins. To understand the mechanism of such complex systems, we need methods to describe molecular arrange-ments and conformations three-dimensionally in vivo. Cryo-electron tomography enabled us such a 3D structural analysis. Our group has been working on 3D structure of flagella/cilia using this method and revealed highly ordered and beautifully organized molecular arrangement. 3D structure gave us insights into the mechanism to gener-ate bending motion with well defined waveforms. In this review, I summarize our recent structural studies on fla-gella/cilia by cryo-electron tomography, mainly focusing on dynein microtubule-based ATPase motor proteins and the radial spoke, a regulatory protein complex. The Biophysical Society of Japan (BSJ) 2013-10-17 /pmc/articles/PMC4629670/ /pubmed/27493552 http://dx.doi.org/10.2142/biophysics.9.141 Text en ©2013 THE BIOPHYSICAL SOCIETY OF JAPAN
spellingShingle Review Article
Ishikawa, Takashi
3D structure of eukaryotic flagella/cilia by cryo-electron tomography
title 3D structure of eukaryotic flagella/cilia by cryo-electron tomography
title_full 3D structure of eukaryotic flagella/cilia by cryo-electron tomography
title_fullStr 3D structure of eukaryotic flagella/cilia by cryo-electron tomography
title_full_unstemmed 3D structure of eukaryotic flagella/cilia by cryo-electron tomography
title_short 3D structure of eukaryotic flagella/cilia by cryo-electron tomography
title_sort 3d structure of eukaryotic flagella/cilia by cryo-electron tomography
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629670/
https://www.ncbi.nlm.nih.gov/pubmed/27493552
http://dx.doi.org/10.2142/biophysics.9.141
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