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3D structure of eukaryotic flagella/cilia by cryo-electron tomography
Flagella/cilia are motile organelles with more than 400 proteins. To understand the mechanism of such complex systems, we need methods to describe molecular arrange-ments and conformations three-dimensionally in vivo. Cryo-electron tomography enabled us such a 3D structural analysis. Our group has b...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Biophysical Society of Japan (BSJ)
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629670/ https://www.ncbi.nlm.nih.gov/pubmed/27493552 http://dx.doi.org/10.2142/biophysics.9.141 |
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author | Ishikawa, Takashi |
author_facet | Ishikawa, Takashi |
author_sort | Ishikawa, Takashi |
collection | PubMed |
description | Flagella/cilia are motile organelles with more than 400 proteins. To understand the mechanism of such complex systems, we need methods to describe molecular arrange-ments and conformations three-dimensionally in vivo. Cryo-electron tomography enabled us such a 3D structural analysis. Our group has been working on 3D structure of flagella/cilia using this method and revealed highly ordered and beautifully organized molecular arrangement. 3D structure gave us insights into the mechanism to gener-ate bending motion with well defined waveforms. In this review, I summarize our recent structural studies on fla-gella/cilia by cryo-electron tomography, mainly focusing on dynein microtubule-based ATPase motor proteins and the radial spoke, a regulatory protein complex. |
format | Online Article Text |
id | pubmed-4629670 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Biophysical Society of Japan (BSJ) |
record_format | MEDLINE/PubMed |
spelling | pubmed-46296702016-08-04 3D structure of eukaryotic flagella/cilia by cryo-electron tomography Ishikawa, Takashi Biophysics (Nagoya-shi) Review Article Flagella/cilia are motile organelles with more than 400 proteins. To understand the mechanism of such complex systems, we need methods to describe molecular arrange-ments and conformations three-dimensionally in vivo. Cryo-electron tomography enabled us such a 3D structural analysis. Our group has been working on 3D structure of flagella/cilia using this method and revealed highly ordered and beautifully organized molecular arrangement. 3D structure gave us insights into the mechanism to gener-ate bending motion with well defined waveforms. In this review, I summarize our recent structural studies on fla-gella/cilia by cryo-electron tomography, mainly focusing on dynein microtubule-based ATPase motor proteins and the radial spoke, a regulatory protein complex. The Biophysical Society of Japan (BSJ) 2013-10-17 /pmc/articles/PMC4629670/ /pubmed/27493552 http://dx.doi.org/10.2142/biophysics.9.141 Text en ©2013 THE BIOPHYSICAL SOCIETY OF JAPAN |
spellingShingle | Review Article Ishikawa, Takashi 3D structure of eukaryotic flagella/cilia by cryo-electron tomography |
title | 3D structure of eukaryotic flagella/cilia by cryo-electron tomography |
title_full | 3D structure of eukaryotic flagella/cilia by cryo-electron tomography |
title_fullStr | 3D structure of eukaryotic flagella/cilia by cryo-electron tomography |
title_full_unstemmed | 3D structure of eukaryotic flagella/cilia by cryo-electron tomography |
title_short | 3D structure of eukaryotic flagella/cilia by cryo-electron tomography |
title_sort | 3d structure of eukaryotic flagella/cilia by cryo-electron tomography |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629670/ https://www.ncbi.nlm.nih.gov/pubmed/27493552 http://dx.doi.org/10.2142/biophysics.9.141 |
work_keys_str_mv | AT ishikawatakashi 3dstructureofeukaryoticflagellaciliabycryoelectrontomography |