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All-Trans Retinoic Acid Has a Potential Therapeutic Role for Diabetic Nephropathy
PURPOSE: The aim of this study was to examine the effects of all-trans retinoic acid (ATRA) on diabetic nephropathy. MATERIALS AND METHODS: We measured amounts of urinary albumin excretion (UAE) after administrating ATRA to Otsuka Long-Evans Tokushima Fatty (OLETF) rats. In order to understand the m...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Yonsei University College of Medicine
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630048/ https://www.ncbi.nlm.nih.gov/pubmed/26446642 http://dx.doi.org/10.3349/ymj.2015.56.6.1597 |
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author | Kim, Chul Sik Park, Jong Suk Ahn, Chul Woo Kim, Kyung Rae |
author_facet | Kim, Chul Sik Park, Jong Suk Ahn, Chul Woo Kim, Kyung Rae |
author_sort | Kim, Chul Sik |
collection | PubMed |
description | PURPOSE: The aim of this study was to examine the effects of all-trans retinoic acid (ATRA) on diabetic nephropathy. MATERIALS AND METHODS: We measured amounts of urinary albumin excretion (UAE) after administrating ATRA to Otsuka Long-Evans Tokushima Fatty (OLETF) rats. In order to understand the mechanism of action for ATRA, we administrated ATRA to examine its inhibitory action on the production of transforming growth factor-β(1) (TGF-β(1)), protein kinase C (PKC), and reactive oxidative stress (ROS) in cultured rat mesangial cells (RMCs). RESULTS: After 16 weeks of treatment, UAE was lower in the ATRA-treated OLETF rats than in the non-treated OLETF rats (0.07±0.03 mg/mgCr vs. 0.17±0.15 mg/mgCr, p<0.01). After incubation of RMCs in media containing 30 or 5 mM of glucose, treatment with ATRA showed time- and dose-dependent decreases in TGF-β(1) levels and ROS. Moreover, ATRA treatment showed a dose-dependent decrease in PKC expression. CONCLUSION: ATRA treatment suppressed UAE and TGF-β(1) synthesis, which was mediated by significant reductions in PKC activity and ROS production. Our results suggest that ATRA has a potential therapeutic role for diabetic nephropathy. |
format | Online Article Text |
id | pubmed-4630048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Yonsei University College of Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-46300482015-11-04 All-Trans Retinoic Acid Has a Potential Therapeutic Role for Diabetic Nephropathy Kim, Chul Sik Park, Jong Suk Ahn, Chul Woo Kim, Kyung Rae Yonsei Med J Original Article PURPOSE: The aim of this study was to examine the effects of all-trans retinoic acid (ATRA) on diabetic nephropathy. MATERIALS AND METHODS: We measured amounts of urinary albumin excretion (UAE) after administrating ATRA to Otsuka Long-Evans Tokushima Fatty (OLETF) rats. In order to understand the mechanism of action for ATRA, we administrated ATRA to examine its inhibitory action on the production of transforming growth factor-β(1) (TGF-β(1)), protein kinase C (PKC), and reactive oxidative stress (ROS) in cultured rat mesangial cells (RMCs). RESULTS: After 16 weeks of treatment, UAE was lower in the ATRA-treated OLETF rats than in the non-treated OLETF rats (0.07±0.03 mg/mgCr vs. 0.17±0.15 mg/mgCr, p<0.01). After incubation of RMCs in media containing 30 or 5 mM of glucose, treatment with ATRA showed time- and dose-dependent decreases in TGF-β(1) levels and ROS. Moreover, ATRA treatment showed a dose-dependent decrease in PKC expression. CONCLUSION: ATRA treatment suppressed UAE and TGF-β(1) synthesis, which was mediated by significant reductions in PKC activity and ROS production. Our results suggest that ATRA has a potential therapeutic role for diabetic nephropathy. Yonsei University College of Medicine 2015-11-01 2015-09-25 /pmc/articles/PMC4630048/ /pubmed/26446642 http://dx.doi.org/10.3349/ymj.2015.56.6.1597 Text en © Copyright: Yonsei University College of Medicine 2015 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Chul Sik Park, Jong Suk Ahn, Chul Woo Kim, Kyung Rae All-Trans Retinoic Acid Has a Potential Therapeutic Role for Diabetic Nephropathy |
title | All-Trans Retinoic Acid Has a Potential Therapeutic Role for Diabetic Nephropathy |
title_full | All-Trans Retinoic Acid Has a Potential Therapeutic Role for Diabetic Nephropathy |
title_fullStr | All-Trans Retinoic Acid Has a Potential Therapeutic Role for Diabetic Nephropathy |
title_full_unstemmed | All-Trans Retinoic Acid Has a Potential Therapeutic Role for Diabetic Nephropathy |
title_short | All-Trans Retinoic Acid Has a Potential Therapeutic Role for Diabetic Nephropathy |
title_sort | all-trans retinoic acid has a potential therapeutic role for diabetic nephropathy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630048/ https://www.ncbi.nlm.nih.gov/pubmed/26446642 http://dx.doi.org/10.3349/ymj.2015.56.6.1597 |
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