Cargando…

Genetic Polymorphism and Expression of CXCR4 in Breast Cancer

CXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients...

Descripción completa

Detalles Bibliográficos
Autores principales: Okuyama Kishima, Marina, Brajão de Oliveira, Karen, Ariza, Carolina Batista, de Oliveira, Carlos Eduardo Coral, Losi Guembarovski, Roberta, Banin Hirata, Bruna Karina, de Almeida, Felipe Campos, Vitiello, Glauco Akelinghton Freire, Trugilo, Kleber Paiva, Guembarovski, Alda Fiorina Maria Losi, Jorge Sobrinho, Walter, Campos, Clodoaldo Zago, Watanabe, Maria Angelica Ehara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630341/
https://www.ncbi.nlm.nih.gov/pubmed/26576337
http://dx.doi.org/10.1155/2015/289510
_version_ 1782398681940492288
author Okuyama Kishima, Marina
Brajão de Oliveira, Karen
Ariza, Carolina Batista
de Oliveira, Carlos Eduardo Coral
Losi Guembarovski, Roberta
Banin Hirata, Bruna Karina
de Almeida, Felipe Campos
Vitiello, Glauco Akelinghton Freire
Trugilo, Kleber Paiva
Guembarovski, Alda Fiorina Maria Losi
Jorge Sobrinho, Walter
Campos, Clodoaldo Zago
Watanabe, Maria Angelica Ehara
author_facet Okuyama Kishima, Marina
Brajão de Oliveira, Karen
Ariza, Carolina Batista
de Oliveira, Carlos Eduardo Coral
Losi Guembarovski, Roberta
Banin Hirata, Bruna Karina
de Almeida, Felipe Campos
Vitiello, Glauco Akelinghton Freire
Trugilo, Kleber Paiva
Guembarovski, Alda Fiorina Maria Losi
Jorge Sobrinho, Walter
Campos, Clodoaldo Zago
Watanabe, Maria Angelica Ehara
author_sort Okuyama Kishima, Marina
collection PubMed
description CXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients presented higher CXCR4 mRNA relative expression (5.7-fold) than normal mammary gland, but this expression was not correlated with patients clinicopathological features (nuclear grade, nodal status, ER status, PR status, p53 staining, Ki67 index, and HER-2 status). Moreover, CXCR4 mRNA relative expression also did not differ regarding the presence or absence of T allele (p = 0.301). In the immunohistochemical assay, no difference was observed for CXCR4 cytoplasmic protein staining in relation to different genotypes (p = 0.757); however, high cytoplasmic CXCR4 staining was verified in invasive breast carcinoma (p < 0.01). All in all, the results from present study indicated that rs2228014 genetic variant does not alter CXCR4 mRNA or protein expression. However, this receptor was more expressed in tumor compared to normal tissue, in both RNA and protein levels, suggesting its promising applicability in the general context of mammary carcinogenesis.
format Online
Article
Text
id pubmed-4630341
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-46303412015-11-16 Genetic Polymorphism and Expression of CXCR4 in Breast Cancer Okuyama Kishima, Marina Brajão de Oliveira, Karen Ariza, Carolina Batista de Oliveira, Carlos Eduardo Coral Losi Guembarovski, Roberta Banin Hirata, Bruna Karina de Almeida, Felipe Campos Vitiello, Glauco Akelinghton Freire Trugilo, Kleber Paiva Guembarovski, Alda Fiorina Maria Losi Jorge Sobrinho, Walter Campos, Clodoaldo Zago Watanabe, Maria Angelica Ehara Anal Cell Pathol (Amst) Research Article CXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients presented higher CXCR4 mRNA relative expression (5.7-fold) than normal mammary gland, but this expression was not correlated with patients clinicopathological features (nuclear grade, nodal status, ER status, PR status, p53 staining, Ki67 index, and HER-2 status). Moreover, CXCR4 mRNA relative expression also did not differ regarding the presence or absence of T allele (p = 0.301). In the immunohistochemical assay, no difference was observed for CXCR4 cytoplasmic protein staining in relation to different genotypes (p = 0.757); however, high cytoplasmic CXCR4 staining was verified in invasive breast carcinoma (p < 0.01). All in all, the results from present study indicated that rs2228014 genetic variant does not alter CXCR4 mRNA or protein expression. However, this receptor was more expressed in tumor compared to normal tissue, in both RNA and protein levels, suggesting its promising applicability in the general context of mammary carcinogenesis. Hindawi Publishing Corporation 2015 2015-10-20 /pmc/articles/PMC4630341/ /pubmed/26576337 http://dx.doi.org/10.1155/2015/289510 Text en Copyright © 2015 Marina Okuyama Kishima et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Okuyama Kishima, Marina
Brajão de Oliveira, Karen
Ariza, Carolina Batista
de Oliveira, Carlos Eduardo Coral
Losi Guembarovski, Roberta
Banin Hirata, Bruna Karina
de Almeida, Felipe Campos
Vitiello, Glauco Akelinghton Freire
Trugilo, Kleber Paiva
Guembarovski, Alda Fiorina Maria Losi
Jorge Sobrinho, Walter
Campos, Clodoaldo Zago
Watanabe, Maria Angelica Ehara
Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
title Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
title_full Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
title_fullStr Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
title_full_unstemmed Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
title_short Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
title_sort genetic polymorphism and expression of cxcr4 in breast cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630341/
https://www.ncbi.nlm.nih.gov/pubmed/26576337
http://dx.doi.org/10.1155/2015/289510
work_keys_str_mv AT okuyamakishimamarina geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT brajaodeoliveirakaren geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT arizacarolinabatista geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT deoliveiracarloseduardocoral geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT losiguembarovskiroberta geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT baninhiratabrunakarina geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT dealmeidafelipecampos geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT vitielloglaucoakelinghtonfreire geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT trugilokleberpaiva geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT guembarovskialdafiorinamarialosi geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT jorgesobrinhowalter geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT camposclodoaldozago geneticpolymorphismandexpressionofcxcr4inbreastcancer
AT watanabemariaangelicaehara geneticpolymorphismandexpressionofcxcr4inbreastcancer