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Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
CXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630341/ https://www.ncbi.nlm.nih.gov/pubmed/26576337 http://dx.doi.org/10.1155/2015/289510 |
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author | Okuyama Kishima, Marina Brajão de Oliveira, Karen Ariza, Carolina Batista de Oliveira, Carlos Eduardo Coral Losi Guembarovski, Roberta Banin Hirata, Bruna Karina de Almeida, Felipe Campos Vitiello, Glauco Akelinghton Freire Trugilo, Kleber Paiva Guembarovski, Alda Fiorina Maria Losi Jorge Sobrinho, Walter Campos, Clodoaldo Zago Watanabe, Maria Angelica Ehara |
author_facet | Okuyama Kishima, Marina Brajão de Oliveira, Karen Ariza, Carolina Batista de Oliveira, Carlos Eduardo Coral Losi Guembarovski, Roberta Banin Hirata, Bruna Karina de Almeida, Felipe Campos Vitiello, Glauco Akelinghton Freire Trugilo, Kleber Paiva Guembarovski, Alda Fiorina Maria Losi Jorge Sobrinho, Walter Campos, Clodoaldo Zago Watanabe, Maria Angelica Ehara |
author_sort | Okuyama Kishima, Marina |
collection | PubMed |
description | CXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients presented higher CXCR4 mRNA relative expression (5.7-fold) than normal mammary gland, but this expression was not correlated with patients clinicopathological features (nuclear grade, nodal status, ER status, PR status, p53 staining, Ki67 index, and HER-2 status). Moreover, CXCR4 mRNA relative expression also did not differ regarding the presence or absence of T allele (p = 0.301). In the immunohistochemical assay, no difference was observed for CXCR4 cytoplasmic protein staining in relation to different genotypes (p = 0.757); however, high cytoplasmic CXCR4 staining was verified in invasive breast carcinoma (p < 0.01). All in all, the results from present study indicated that rs2228014 genetic variant does not alter CXCR4 mRNA or protein expression. However, this receptor was more expressed in tumor compared to normal tissue, in both RNA and protein levels, suggesting its promising applicability in the general context of mammary carcinogenesis. |
format | Online Article Text |
id | pubmed-4630341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46303412015-11-16 Genetic Polymorphism and Expression of CXCR4 in Breast Cancer Okuyama Kishima, Marina Brajão de Oliveira, Karen Ariza, Carolina Batista de Oliveira, Carlos Eduardo Coral Losi Guembarovski, Roberta Banin Hirata, Bruna Karina de Almeida, Felipe Campos Vitiello, Glauco Akelinghton Freire Trugilo, Kleber Paiva Guembarovski, Alda Fiorina Maria Losi Jorge Sobrinho, Walter Campos, Clodoaldo Zago Watanabe, Maria Angelica Ehara Anal Cell Pathol (Amst) Research Article CXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients presented higher CXCR4 mRNA relative expression (5.7-fold) than normal mammary gland, but this expression was not correlated with patients clinicopathological features (nuclear grade, nodal status, ER status, PR status, p53 staining, Ki67 index, and HER-2 status). Moreover, CXCR4 mRNA relative expression also did not differ regarding the presence or absence of T allele (p = 0.301). In the immunohistochemical assay, no difference was observed for CXCR4 cytoplasmic protein staining in relation to different genotypes (p = 0.757); however, high cytoplasmic CXCR4 staining was verified in invasive breast carcinoma (p < 0.01). All in all, the results from present study indicated that rs2228014 genetic variant does not alter CXCR4 mRNA or protein expression. However, this receptor was more expressed in tumor compared to normal tissue, in both RNA and protein levels, suggesting its promising applicability in the general context of mammary carcinogenesis. Hindawi Publishing Corporation 2015 2015-10-20 /pmc/articles/PMC4630341/ /pubmed/26576337 http://dx.doi.org/10.1155/2015/289510 Text en Copyright © 2015 Marina Okuyama Kishima et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Okuyama Kishima, Marina Brajão de Oliveira, Karen Ariza, Carolina Batista de Oliveira, Carlos Eduardo Coral Losi Guembarovski, Roberta Banin Hirata, Bruna Karina de Almeida, Felipe Campos Vitiello, Glauco Akelinghton Freire Trugilo, Kleber Paiva Guembarovski, Alda Fiorina Maria Losi Jorge Sobrinho, Walter Campos, Clodoaldo Zago Watanabe, Maria Angelica Ehara Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
title | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
title_full | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
title_fullStr | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
title_full_unstemmed | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
title_short | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
title_sort | genetic polymorphism and expression of cxcr4 in breast cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630341/ https://www.ncbi.nlm.nih.gov/pubmed/26576337 http://dx.doi.org/10.1155/2015/289510 |
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