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High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research
Imaging techniques for visualizing cerebral vasculature and distinguishing functional areas are essential and critical to the study of various brain diseases. In this paper, with the X-ray phase-contrast imaging technique, we proposed an experiment scheme for the ex vivo mouse brain study, achieving...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630379/ https://www.ncbi.nlm.nih.gov/pubmed/26576198 http://dx.doi.org/10.1155/2015/530580 |
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author | Xi, Yan Lin, Xiaojie Yuan, Falei Yang, Guo-Yuan Zhao, Jun |
author_facet | Xi, Yan Lin, Xiaojie Yuan, Falei Yang, Guo-Yuan Zhao, Jun |
author_sort | Xi, Yan |
collection | PubMed |
description | Imaging techniques for visualizing cerebral vasculature and distinguishing functional areas are essential and critical to the study of various brain diseases. In this paper, with the X-ray phase-contrast imaging technique, we proposed an experiment scheme for the ex vivo mouse brain study, achieving both high spatial resolution and improved soft-tissue contrast. This scheme includes two steps: sample preparation and volume reconstruction. In the first step, we use heparinized saline to displace the blood inside cerebral vessels and then replace it with air making air-filled mouse brain. After sample preparation, X-ray phase-contrast tomography is performed to collect the data for volume reconstruction. Here, we adopt a phase-retrieval combined filtered backprojection method to reconstruct its three-dimensional structure and redesigned the reconstruction kernel. To evaluate its performance, we carried out experiments at Shanghai Synchrotron Radiation Facility. The results show that the air-tissue structured cerebral vasculatures are highly visible with propagation-based phase-contrast imaging and can be clearly resolved in reconstructed cross-images. Besides, functional areas, such as the corpus callosum, corpus striatum, and nuclei, are also clearly resolved. The proposed method is comparable with hematoxylin and eosin staining method but represents the studied mouse brain in three dimensions, offering a potential powerful tool for the research of brain disorders. |
format | Online Article Text |
id | pubmed-4630379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46303792015-11-16 High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research Xi, Yan Lin, Xiaojie Yuan, Falei Yang, Guo-Yuan Zhao, Jun Comput Math Methods Med Research Article Imaging techniques for visualizing cerebral vasculature and distinguishing functional areas are essential and critical to the study of various brain diseases. In this paper, with the X-ray phase-contrast imaging technique, we proposed an experiment scheme for the ex vivo mouse brain study, achieving both high spatial resolution and improved soft-tissue contrast. This scheme includes two steps: sample preparation and volume reconstruction. In the first step, we use heparinized saline to displace the blood inside cerebral vessels and then replace it with air making air-filled mouse brain. After sample preparation, X-ray phase-contrast tomography is performed to collect the data for volume reconstruction. Here, we adopt a phase-retrieval combined filtered backprojection method to reconstruct its three-dimensional structure and redesigned the reconstruction kernel. To evaluate its performance, we carried out experiments at Shanghai Synchrotron Radiation Facility. The results show that the air-tissue structured cerebral vasculatures are highly visible with propagation-based phase-contrast imaging and can be clearly resolved in reconstructed cross-images. Besides, functional areas, such as the corpus callosum, corpus striatum, and nuclei, are also clearly resolved. The proposed method is comparable with hematoxylin and eosin staining method but represents the studied mouse brain in three dimensions, offering a potential powerful tool for the research of brain disorders. Hindawi Publishing Corporation 2015 2015-10-20 /pmc/articles/PMC4630379/ /pubmed/26576198 http://dx.doi.org/10.1155/2015/530580 Text en Copyright © 2015 Yan Xi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xi, Yan Lin, Xiaojie Yuan, Falei Yang, Guo-Yuan Zhao, Jun High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research |
title | High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research |
title_full | High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research |
title_fullStr | High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research |
title_full_unstemmed | High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research |
title_short | High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research |
title_sort | high-resolution and quantitative x-ray phase-contrast tomography for mouse brain research |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630379/ https://www.ncbi.nlm.nih.gov/pubmed/26576198 http://dx.doi.org/10.1155/2015/530580 |
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