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Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries
Culturing Mycobacterium tuberculosis remains the gold standard for the laboratory diagnosis of pulmonary tuberculosis, with 9 million new cases and 1.5 million deaths mainly in developing countries. Reviewing data reported over 20 years yields a state-of-the-art procedure for the routine culture of...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630581/ https://www.ncbi.nlm.nih.gov/pubmed/26579092 http://dx.doi.org/10.3389/fmicb.2015.01184 |
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author | Asmar, Shady Drancourt, Michel |
author_facet | Asmar, Shady Drancourt, Michel |
author_sort | Asmar, Shady |
collection | PubMed |
description | Culturing Mycobacterium tuberculosis remains the gold standard for the laboratory diagnosis of pulmonary tuberculosis, with 9 million new cases and 1.5 million deaths mainly in developing countries. Reviewing data reported over 20 years yields a state-of-the-art procedure for the routine culture of M. tuberculosis in both developed and developing countries. Useful specimens include sputum, induced sputum, and stools collected in quaternary ammonium preservative-containing sterile cans. The usefulness of other non-invasive specimens remains to be evaluated. Specimens can be collected in a diagnosis kit also containing sampling materials, instructions, laboratory requests, and informed consent. Automated direct LED fluorescence microscopy after auramine staining precedes inoculation of an egg-lecithin-containing culture solid medium under microaerophilic atmosphere, inverted microscope reading or scanning video-imaging detection of colonies and colonies identification by recent molecular methods. This procedure should result in a diagnosis of pulmonary tuberculosis as fast as 5 days. It may be implemented in both developed and developing countries with automated steps replaceable by manual steps depending on local resources. |
format | Online Article Text |
id | pubmed-4630581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46305812015-11-17 Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries Asmar, Shady Drancourt, Michel Front Microbiol Public Health Culturing Mycobacterium tuberculosis remains the gold standard for the laboratory diagnosis of pulmonary tuberculosis, with 9 million new cases and 1.5 million deaths mainly in developing countries. Reviewing data reported over 20 years yields a state-of-the-art procedure for the routine culture of M. tuberculosis in both developed and developing countries. Useful specimens include sputum, induced sputum, and stools collected in quaternary ammonium preservative-containing sterile cans. The usefulness of other non-invasive specimens remains to be evaluated. Specimens can be collected in a diagnosis kit also containing sampling materials, instructions, laboratory requests, and informed consent. Automated direct LED fluorescence microscopy after auramine staining precedes inoculation of an egg-lecithin-containing culture solid medium under microaerophilic atmosphere, inverted microscope reading or scanning video-imaging detection of colonies and colonies identification by recent molecular methods. This procedure should result in a diagnosis of pulmonary tuberculosis as fast as 5 days. It may be implemented in both developed and developing countries with automated steps replaceable by manual steps depending on local resources. Frontiers Media S.A. 2015-11-03 /pmc/articles/PMC4630581/ /pubmed/26579092 http://dx.doi.org/10.3389/fmicb.2015.01184 Text en Copyright © 2015 Asmar and Drancourt. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Public Health Asmar, Shady Drancourt, Michel Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries |
title | Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries |
title_full | Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries |
title_fullStr | Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries |
title_full_unstemmed | Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries |
title_short | Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries |
title_sort | rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries |
topic | Public Health |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630581/ https://www.ncbi.nlm.nih.gov/pubmed/26579092 http://dx.doi.org/10.3389/fmicb.2015.01184 |
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