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pH and redox sensitive albumin hydrogel: A self-derived biomaterial
Serum albumin can be transformed to a stimuli (pH and redox) responsive hydrogel using the reduction process followed by oxidative refolding. The preparation of albumin hydrogel involves a range of concentrations (75, 150, 300, 450, 600 and 750 μM) and pH (2.0–10.0) values and the gelation begins at...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630586/ https://www.ncbi.nlm.nih.gov/pubmed/26527296 http://dx.doi.org/10.1038/srep15977 |
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author | Raja, S Thirupathi Kumara Thiruselvi, T Mandal, Asit Baran Gnanamani, A |
author_facet | Raja, S Thirupathi Kumara Thiruselvi, T Mandal, Asit Baran Gnanamani, A |
author_sort | Raja, S Thirupathi Kumara |
collection | PubMed |
description | Serum albumin can be transformed to a stimuli (pH and redox) responsive hydrogel using the reduction process followed by oxidative refolding. The preparation of albumin hydrogel involves a range of concentrations (75, 150, 300, 450, 600 and 750 μM) and pH (2.0–10.0) values and the gelation begins at a concentration of 150 μM and 4.5–8.0 pH value. The hydrogel shows maximum swelling at alkali pH (pH > 9.0). The increase in albumin concentration increases hydrogel stability, rheological property, compressive strength, proteolytic resistance and rate of in vivo biodegradation. Based on the observed physical and biological properties of albumin hydrogel, 450 μM was determined to be an optimum concentration for further experiments. In addition, the hemo- and cytocompatibility analyses revealed the biocompatibility nature of albumin hydrogel. The experiments on in vitro drug (Tetracycline) delivery were carried out under non reducing and reducing conditions that resulted in the sustained and fast release of the drug, respectively. The methodology used in the preparation of albumin hydrogel may lead to the development of autogenic tissue constructs. In addition, the methodology can have various applications in tissue engineering and drug delivery. |
format | Online Article Text |
id | pubmed-4630586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46305862015-11-05 pH and redox sensitive albumin hydrogel: A self-derived biomaterial Raja, S Thirupathi Kumara Thiruselvi, T Mandal, Asit Baran Gnanamani, A Sci Rep Article Serum albumin can be transformed to a stimuli (pH and redox) responsive hydrogel using the reduction process followed by oxidative refolding. The preparation of albumin hydrogel involves a range of concentrations (75, 150, 300, 450, 600 and 750 μM) and pH (2.0–10.0) values and the gelation begins at a concentration of 150 μM and 4.5–8.0 pH value. The hydrogel shows maximum swelling at alkali pH (pH > 9.0). The increase in albumin concentration increases hydrogel stability, rheological property, compressive strength, proteolytic resistance and rate of in vivo biodegradation. Based on the observed physical and biological properties of albumin hydrogel, 450 μM was determined to be an optimum concentration for further experiments. In addition, the hemo- and cytocompatibility analyses revealed the biocompatibility nature of albumin hydrogel. The experiments on in vitro drug (Tetracycline) delivery were carried out under non reducing and reducing conditions that resulted in the sustained and fast release of the drug, respectively. The methodology used in the preparation of albumin hydrogel may lead to the development of autogenic tissue constructs. In addition, the methodology can have various applications in tissue engineering and drug delivery. Nature Publishing Group 2015-11-03 /pmc/articles/PMC4630586/ /pubmed/26527296 http://dx.doi.org/10.1038/srep15977 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Raja, S Thirupathi Kumara Thiruselvi, T Mandal, Asit Baran Gnanamani, A pH and redox sensitive albumin hydrogel: A self-derived biomaterial |
title | pH and redox sensitive albumin hydrogel: A self-derived biomaterial |
title_full | pH and redox sensitive albumin hydrogel: A self-derived biomaterial |
title_fullStr | pH and redox sensitive albumin hydrogel: A self-derived biomaterial |
title_full_unstemmed | pH and redox sensitive albumin hydrogel: A self-derived biomaterial |
title_short | pH and redox sensitive albumin hydrogel: A self-derived biomaterial |
title_sort | ph and redox sensitive albumin hydrogel: a self-derived biomaterial |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630586/ https://www.ncbi.nlm.nih.gov/pubmed/26527296 http://dx.doi.org/10.1038/srep15977 |
work_keys_str_mv | AT rajasthirupathikumara phandredoxsensitivealbuminhydrogelaselfderivedbiomaterial AT thiruselvit phandredoxsensitivealbuminhydrogelaselfderivedbiomaterial AT mandalasitbaran phandredoxsensitivealbuminhydrogelaselfderivedbiomaterial AT gnanamania phandredoxsensitivealbuminhydrogelaselfderivedbiomaterial |