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Ontogenetic development of the auditory sensory organ in zebrafish (Danio rerio): changes in hearing sensitivity and related morphology

Zebrafish (Danio rerio) is an important model organism in hearing research. However, data on the hearing sensitivity of zebrafish vary across different reports. In the present study, the hearing sensitivity of zebrafish was examined by analysing the auditory evoked potentials (AEPs) over a range of...

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Autores principales: Wang, Jiping, Song, Qiang, Yu, Dongzhen, Yang, Guang, Xia, Li, Su, Kaiming, Shi, Haibo, Wang, Jian, Yin, Shankai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630651/
https://www.ncbi.nlm.nih.gov/pubmed/26526229
http://dx.doi.org/10.1038/srep15943
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author Wang, Jiping
Song, Qiang
Yu, Dongzhen
Yang, Guang
Xia, Li
Su, Kaiming
Shi, Haibo
Wang, Jian
Yin, Shankai
author_facet Wang, Jiping
Song, Qiang
Yu, Dongzhen
Yang, Guang
Xia, Li
Su, Kaiming
Shi, Haibo
Wang, Jian
Yin, Shankai
author_sort Wang, Jiping
collection PubMed
description Zebrafish (Danio rerio) is an important model organism in hearing research. However, data on the hearing sensitivity of zebrafish vary across different reports. In the present study, the hearing sensitivity of zebrafish was examined by analysing the auditory evoked potentials (AEPs) over a range of total lengths (TLs) from 12 to 46 mm. Morphological changes in the hair cells (HCs) of the saccule (the main auditory end organ) and their synapses with primary auditory neurons were investigated. The AEPs were detected up to a much higher frequency limit (12 kHz) than previously reported. No significant difference in the frequency response range was observed across the TL range examined. However, the AEP thresholds demonstrated both developmental improvement and age-related loss of hearing sensitivity. The changes in hearing sensitivity were roughly consistent with the morphological changes in the saccule including (1) the number and density of HCs, (2) the organization of stereocilia, and (3) the quantity of a main ribbon protein, Ribeye b. The results of this study established a clear baseline for the hearing ability of zebrafish and revealed that the changes in the saccule contribute to the observed changes in TL (age)-related hearing sensitivity.
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spelling pubmed-46306512015-11-16 Ontogenetic development of the auditory sensory organ in zebrafish (Danio rerio): changes in hearing sensitivity and related morphology Wang, Jiping Song, Qiang Yu, Dongzhen Yang, Guang Xia, Li Su, Kaiming Shi, Haibo Wang, Jian Yin, Shankai Sci Rep Article Zebrafish (Danio rerio) is an important model organism in hearing research. However, data on the hearing sensitivity of zebrafish vary across different reports. In the present study, the hearing sensitivity of zebrafish was examined by analysing the auditory evoked potentials (AEPs) over a range of total lengths (TLs) from 12 to 46 mm. Morphological changes in the hair cells (HCs) of the saccule (the main auditory end organ) and their synapses with primary auditory neurons were investigated. The AEPs were detected up to a much higher frequency limit (12 kHz) than previously reported. No significant difference in the frequency response range was observed across the TL range examined. However, the AEP thresholds demonstrated both developmental improvement and age-related loss of hearing sensitivity. The changes in hearing sensitivity were roughly consistent with the morphological changes in the saccule including (1) the number and density of HCs, (2) the organization of stereocilia, and (3) the quantity of a main ribbon protein, Ribeye b. The results of this study established a clear baseline for the hearing ability of zebrafish and revealed that the changes in the saccule contribute to the observed changes in TL (age)-related hearing sensitivity. Nature Publishing Group 2015-11-03 /pmc/articles/PMC4630651/ /pubmed/26526229 http://dx.doi.org/10.1038/srep15943 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Jiping
Song, Qiang
Yu, Dongzhen
Yang, Guang
Xia, Li
Su, Kaiming
Shi, Haibo
Wang, Jian
Yin, Shankai
Ontogenetic development of the auditory sensory organ in zebrafish (Danio rerio): changes in hearing sensitivity and related morphology
title Ontogenetic development of the auditory sensory organ in zebrafish (Danio rerio): changes in hearing sensitivity and related morphology
title_full Ontogenetic development of the auditory sensory organ in zebrafish (Danio rerio): changes in hearing sensitivity and related morphology
title_fullStr Ontogenetic development of the auditory sensory organ in zebrafish (Danio rerio): changes in hearing sensitivity and related morphology
title_full_unstemmed Ontogenetic development of the auditory sensory organ in zebrafish (Danio rerio): changes in hearing sensitivity and related morphology
title_short Ontogenetic development of the auditory sensory organ in zebrafish (Danio rerio): changes in hearing sensitivity and related morphology
title_sort ontogenetic development of the auditory sensory organ in zebrafish (danio rerio): changes in hearing sensitivity and related morphology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630651/
https://www.ncbi.nlm.nih.gov/pubmed/26526229
http://dx.doi.org/10.1038/srep15943
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